| Project/Area Number |
62570830
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University, |
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Principal Investigator |
ASADA Akira Faculty of Dentistry, Instructor, Osaka University, 歯学部, 講師 (50028734)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Fujio Faculty of Dentistry, Professor, Osaka University, 歯学部, 教授 (40028717)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Chondrocytes / Parathyroid hormon / Ornithin decarboxylase / Cyclic AMP / Pulsed electromagnetic field / Pulsed electric field / Parathyroid hormone / Ornithin decaroboxylase / Cell spesificity / Cyclo AMP / Paratiroid trormon / DNA syntheris |
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| Research Abstract |
Previous studies have shown that pulsed electromagnetic field (PEMF) stimulation potentiates parathyroid hormone(PTH) stimulations of cAMP, ornithine decarboxylase and glucosaminoglycan syntheses in primary cultures of rabbit costal chondrocytes.
This indicates that PEMF stimulation angments the sensitivity of cultured cartilage cells to PTH and promotes their differentiation. However, PEMF had little effect on chondrocyte proliferation. The effect of PEMF stimulation was observed only in PTH. It did not give any effects in the presence of cartilage derived factor, fibroblast growth factor, substance-P, and insulin-like growth factor. In the present study, the effect of pulsed electric field(PEF) stimulation on cell proliferation was study using Normal rat kidney cells. PEF stimulation caused 30% increase in the incorporation of <@D13@>D3H-thymidine into DNA in the presence of 0.3% FCS. Further increase in the concentration of FCS accelerated the proliferation of cells and diminished the effect of the PET stimulation. Furthermore the PEF and PEMF stimulations affected alkaline phosphatase activity during the process of calcification in pellet cultures of chondrocytes.
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