| Project/Area Number |
62570839
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | School of Dentistry, Meikai University |
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Principal Investigator |
HIRAMATSU Masahiko School of Dentistry, Meikai University, 歯学部, 助教授 (80049395)
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| Co-Investigator(Kenkyū-buntansha) |
KASHIMATA Masanori School of Dentistry, Meikai University, 歯学部, 助手 (30152630)
MINAMI Naomi School of Dentistry, Meikai University, 歯学部, 教授 (20049349)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Epidermal growth factor (EGF) / EGF receptor / Submandibular gland / Prostate / Diabetes / Sex difference / Growth hormone / 筋ジストロフィー / Enzyme immunoassay / 消化管 / 肝臓 |
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| Research Abstract |
1. We purified a sufficient amount of epidermal growth growth factor (EGF) from male mouse submandibular glands and prepared its specific antibody for the establisment of a sensitive quantitative method for EGF concentration.
2. EGF was widely distributed in a variety of mouse organs although its concentrations were extremely lower as compared with that of submandibular gland. Moreover, EGF in the prostate was found to be androgen dependent.
3. Streptozotocin-induced diabetes did not influence EGF concentration in the rat liver, but did cause a significant decrease the number of EGF receptors. The treatment of diabetic rats with insulin caused an increase in the receptor number to the level of normal rats.
4. In addition, we found a marked sex difference in the number of EGF receptor in the rat liver (the number was about 2-fold higher in males than in females). Furthermore, such sex difference in the umber of EGF receptors, observed here, was under the regulation of hypothalamo-pituitary system, and neonatal androgens were thought to be essential for this regulation, probably through their effects on hypothalamus. Our subsequent study has demonstrated that the number of hepatic EGF receptors in the rat is regulated by the differential secretory rhythm of pituitary growth hormone between males and females.
5. We found the presence of EGF receptors in the rat brain and that the number of the receptors decreased gradually with progressive age.
6. We compared EGF concentration in the skeletal muscle of normal (C57BL/6J) and muscular dystrophic mice (C57BL/6J, dy/dy). In the dystrophic mice, EGF concentration was found to be significantly lower when compared with the control level.
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