| Project/Area Number |
62570912
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TAKAGI Yuzo Associate Professor, Dept. of Pedodontics, School of Dentistry Tokyo Medical and Dental University, 歯学部, 助教授 (30124697)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Junji Instructor, Dept. of Pedodontics, School of Dentistry Tokyo Medical and Dental U, 歯学部, 助手 (80177451)
TANAKA Mitsuro Instructor, Dept. of Pedodontics, School of Dentistry Tokyo Medical and Dental U, 歯学部, 助手 (10143596)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Pulpotomy / Induction / Dentin-bridge / Phosphophoryn / _2HS glycoprotein / 移植 / 生活断髄 / 象牙質 / 石灰化 |
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| Research Abstract |
Non-collagenous matrix of dentin has been thought to play important role in the process of dentin mineralization. In this study, we determined whether non-collagenous dentin matrix can induce hard tissue formation in the subcutaneous tissue of experimental animals.
When tissue distributions of dentin phosphophoryn and _2HS glycoprotein were examined by histochemical or immunohistochemical method, it was found that both of them were present in dentin being strongly associated with mineral crystals. The data suggested that these proteins might play role in the process of mineralization. Then, induction of hard tissue formation was attempted by subcutaneous implantation of these proteins into rats. When polyacrylamide gel disc containing dentin phosphophoryn were subcutaneously implanted into rat, mineral crystals were fromed in the gel disc. These results suggest that phosphophoryn may be able to induce the formation of dentin-bridge when it is placed on the surface of dental pulp after pulpotomy.
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