| Project/Area Number |
62570999
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Faculty of Pharmaceutical Sciences, Teikyo University |
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Principal Investigator |
NISHIGORI Hideo Faculty of pharmaceutical sciences, Teikyo University, 薬学部, 助教授 (90050517)
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| Co-Investigator(Kenkyū-buntansha) |
AOYAMA Kazu Faculty of pharmaceutical sciences, Teikyo University, 薬学部, 助手 (50167798)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | chick embryo / cataract / glucocorticoid / anticataract agent / glutathione / ascorbic acid / lipidperoxide / PQQ / 病態モデル / イソクエン酸 / トリカルボン酸サイクル / 薬効評価系 / レーザーラマン / 水晶体 / ラジカルスカベンジャー |
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| Research Abstract |
Cataract is observed as one of the adverse effects of long-term glucocorticoid (GC) therapy. However, unsuccessful attempts have been made to produce cataract in animal by GC. This present research demonstrates that the administration of GC to 15-day old chick embryos can produce nuclear cataract rapidly and with high incidence. With cataract formation the level of glutathione and ascordic acid in the lenses decreased and that of glucose and lipidperoxide increased.
The cataract formation was effectively prevented by the treatment of radical scavengers, thiopronine, ascordic acid, tocopherol and pyrroloquinoline quinone. The administration of isocitrate prevented the cataract formation, the decline of glutathione of lens and liver, also it prevented the elevation of lipidperoxide of lens, liver and blood. The present work on chick embryo would be a valuable model system for studying the mechanism of cataract formation and for searching anticataract agents.
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