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Structure and function of guanylate cyclase

Research Project

Project/Area Number 62571008
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionTokyo Metropolitan Institute for Neurosciences

Principal Investigator

NAKANE Masaki  Scientific Staff, Department of Molecular Neurobiology Tokyo Metropolitan Institute for Neurosciences, 分子神経生物, 主任研究 (50100144)

Co-Investigator(Kenkyū-buntansha) SASAKI Yukiko  Scientific Staff, Department of Molecular Neurobiology Tokyo Metropolitan Instit, 分子神経生物, 主事研究員 (30142152)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsguanylate cyclase / cyclic GMP / molecular cloning / サブユニット構造 / cDNAクローニング / 活性調節
Research Abstract

Cyclic GMP has been implicated as an intracellular messenger in various tissues, including smooth muscle and retina. The synthesis of cyclic GMP is catalyzed by guanylate cyclase. In most tissues, the enzyme is present in both soluble and membrane-bound forms, and is regulated independently during tissue proliferation and differentiation. However, definitive studies will require characterization of the purified protein or the genes encoding these enzymes.
To obtain a detailed analysis of the structural features of guanylate cyclase, a cDNA clone corresponding to the 70kDa subunit of soluble guanylate cyclase of rat lung has been isolated. The primary structure of the cDNA consisted of 3063 nucleotides including a 1857-nucleotide coding region for 619 amino acids, and calculated molelcular weight was 70476. The amount of MRNA was abundant in lung, cerebrum and cerebellum, moderate in heart and kidney, and low in liver and muscle. The amino acid sequence of the 70kDa subunit has partial homology with particulate guanylate cyclase from sea-urchin sperm, and protein phosphatase inhibitor I.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Masaki Nakane: Biochemical and Biophysical Research Communications. 157. 1139-1147 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中根正樹: "医薬品の開発,第8巻 薬物の作用機構二次メッセンジャーとしての cGNP" 広川書店, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masaki Nakane; Shuichi Saheki; Takayoshi Kuno; Kuniaki Ishii; Ferid Murad: "Molecular cloning of a cDNA coding for 70 kilodalton subunit of soluble guanylate cyclase from rat lung." Biochemical and Biophysical Research Communications. 157. 1139-1147 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masaki Nakane: Cyclic GMP as a second messenger. Hirokawa Shoten, Tokyo, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masaki Nakane.: Biochemical and Biophysical Research Communications. 157. 1139-1147 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中根正樹: "医薬品の開発、第8巻 薬物の作用機構 二次メッセンジャーとしてのcGMP" 広川書店, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nakane,M. et.al.: Proc. Natl. Acad. Sci.,U.S.A.(1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 鈴木郁生 他: "医学品の開発 第8巻「薬物の作用機構」" 廣川書店, (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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