Isolation and characterization of retina specific cDNA clones
| Project/Area Number |
62571022
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | Cancer Research Institute, Kanazawa University |
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Principal Investigator |
KUO Che-Hui Cancer Research Institute, Research associate, がん研究所, 助手 (50126570)
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| Co-Investigator(Kenkyū-buntansha) |
MIKI Naomasa Cancer Research Institute, Professor, がん研究所, 教授 (40094445)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | MEKA cDNA / MEKA protein / Retina / Photoreceptor cell / 組織化学 / cDNAクローニング / 光受容細胞 / ホスフォジエステラーゼ |
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| Research Abstract |
We have isolated a MEKA cDNA clone by differential colony hybridization method. The MEKA cDNA encodes unknown soluble protein whose molecular weight and pI are calculated to be 27KDa and 5.35, respectively. We found by Northern and in situ hybridization that MEKA mRNA was transcribed only in the photoreceptor cells. The MEKA cDNA was ligated with expression vector PEX1 and a MEKA-fusion protein synthesized in E.coli was purified and used as antigen. By the Western blot and immunohistochemical study, we found that MEKA protein is expressed in the photoreceptor cells, but not in other tissues.
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Report
(3 results)
Research Products
(17 results)
