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STUDIES FOR THE ROLES OF OPIOID PEPTIDE IN BODY FLUID

Research Project

Project/Area Number 62571031
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用薬理学・医療系薬学
Research InstitutionTHE TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE

Principal Investigator

HAZATO TADAHIKO  THE TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE PRINCIPAL SCIENTIST, 主任研究員 (60109949)

Co-Investigator(Kenkyū-buntansha) KAYA KEIJI  JUNTENDO SCHOOL OF MEDICINE, 麻酔学, 教授 (20124969)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsOPIOIDE PEPTIDE / LEUCINE-ENKEPHALIN / CEREBROSPINAL FLUID / SERUM / PAIN / 妊娠 / 動態 / オピオイド・ペプチド / オペオイド・ペプチド / ペプチデース
Research Abstract

A radioimmunoassy method for endogenous leusine-enkephalin in human body fluid is developed and used to study the immunoreactive components. This method was different from the conventional method up to the step of extraction immunoreactive leucine-enkephalin. Namely, the influence of the discarded proteins in body fluid can be removed by dialysis. The dialytic outer solution containing enkephalin is applied on silicagel ODS-Q3 column and further examined by means of HPLC combined with a specific radioimmunoassay method. In concequence, the mean value of leucine enkephalin like substance in normal human cerebrospinal fluid and serum were 423 pg/ml and 174 pg/ml. The enkephalin like immunoreactivity in cerebrospinal fluid of patients were measured and divided into three groups, normal control group, acute pain group and chronic pain group. The values in control group, acute pain group and chronic pain group were 365 pg/ml,1121 pg/ml and 115 pg/ml, respectively. These results indicate that acute pain stress may release enkephalin into cerebrospinal fluid, which may modify pain sensation and chronic pain stress may decrease enkephalin, enhancing pain sensation. In the one hand, serum levels of leucin-enkephalin were determined 26 woman who had become pregnant normally, some of whom 5 to 40 weeks gestation. Enkephalin dose during 5-6 weeks gestation 982 pg/ml, differing significantly from that of the normal adult control groups, 174 pg/ml. The values of serum enkephalin during 10-40 weeks of gestation were within normal limits. These results indicate that the enkephalin concentrations increase in earyly stage of pregnancy. Although the physiological mechanism and exact meaning of this change is still unknown, a srong possibility suggests itself that this substance has an important part to play in such physiological functions as pregnancy.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Kobayashi,S.;Kaya,K.;Kasama,A.;Hazato,H.et al: Jap.Anaethesia.J.Review. 2. 127-130 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakajima,S.;Kaya,K.;Hazato,T.: Neuropeptide. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakajima,S.;Komuro,T.;Hazato,T.: Biochem.Int. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 小林澄子、羽里忠彦: 麻酔. 36. 857-861 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 蓮見謙司、島村真里子、羽里忠彦: 麻酔. 37. 340-343 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 蓮見謙司、羽里忠彦: 麻酔. 37. 798-803 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 羽里忠彦: "エンケファリン分解酵素と阻害物質、続脳の生体警告系ー不安、不快、痛みの機構" 東京大学出版会, 14 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] KOBAYASHI,S.; KAYA,K.;KASAMA,A.;HAZATO,T.; KATAYAMA,T: "ENKEPHALIN DEGRADING ENZYMES AND THEIR ENDOGENOUS INHIBITOR IN HUMAN CEREBROSPINAL FLUID" JAP,ANAESTHESIA,J,REVIEW. 2. 127-130 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] HASUMI,K.;SHIMAMURA,M.;HAZATO,T: "EVALUATION OF ENDOGENOUS LEUCINE-ENKEPHALIN LIKE SUBSTANCE IN HUMAN CEREBROSPINAL FLUID" JAP,J,ANAESTHESIOLOGY. 37. 340-343 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] HASUMI,K.;HAZATO,T: "EVALUATION OF ENDOGENOUS LEUCINE-ENKEPHALIN LIKE SUBSTANCE IN HUMAN CEREBROSPINAL FLUID-PART 2-" JAP,J,ANAESTHESIOLOGY. 37. 798-803 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] NAKAJIMA,S.;KAYA,K.;HAZATO,T: "PROTECTION OF ENDOGEOUS ENKEPHALIN DEGRADATION FROM PEPTIDASES IN HUMAN SERUM BY ACTINONIN" NEUROPEPTIDE. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] NAKAJIMA,S.;KOMURO,T.;HAZATO,T: "ENKEPHALIN-BINDING PROTEIN IN HUMAN BLOOD" BIOCHEM,INT. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] HAZATO,T: ENKEPHALIN DEGRADING ENZYMES AND ITS INHIBITOR. UNIVERSTY OF TOKYO PRESS, 14 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kobayashi,S.,;Kaya,K.,;Kasama,A.,;Hazato,H.,etal: Jap.Anaethesia.J.Review. 2. 127-130 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nakajima,S.,;Kaya,K;Hazato,T.: Neuropeptide. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nakajima,S.,;Komuro,T;Hazato,T.: Biochem,Int. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 小林澄子、羽里忠彦: 麻酔. 36. 857-861 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 蓮見謙司、島村真理子、羽里忠彦: 麻酔. 36. 340-343 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 蓮見謙司、羽里忠彦: 麻酔. 37. 798-803 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 羽里忠彦: "エンケファリン分解酵素と阻害物質、続脳の生体警告系-不安、不快、痛みの機構" 東京大学出版会, 14 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 蓮見謙司,島村真里子,羽里忠彦: Jap.J. Anaesthesiology. 37. 340-343 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 小林澄子,茅 二,笠間晃夫,羽里忠彦: Jap. Anaesthesia. J. Review. IN PRESS (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 羽里忠彦,島村真里子,西村欣也,茅 二: Clinical Biochemistry 投稿予定.

    • Related Report
      1987 Annual Research Report
  • [Publications] 羽里忠彦,島村真里子,茅 二: 鎮痛薬・オピオイドペプチドシンポジウム. 8. 27-28 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 羽里 忠彦: Sut Bulletin (東京理科大学出版会). 5. 38-42 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 羽里 忠彦: "続脳の生体警告系-不安・不快痛みの機構 エンケファリン分解酵素と阻害剤" 東京大学出版会, 193-207 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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