| Project/Area Number |
62571039
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Showa University |
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Principal Investigator |
GOMI Kunihide Showa University, School of Medicine, Professor, 医学部, 教授 (60053980)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Hiroshi Showa University, School of Medicine, Assistant, 医学部, 助手 (80211308)
SUZUKI Toshiyuki Showa University, School of Medicine, Assistant, 医学部, 助手
MIYA Tetsumasa Showa University, School of Medicine, Assistant, 医学部, 助手 (40201800)
UZAWA Ryuichi Showa University, School of Medicine, Lecturer, 医学部, 講師 (80160232)
TAKAGI Yasushi Showa University, School of Medicine, Assistant Professor, 医学部, 助教授 (30138490)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | mitochondrial CK(m-CK) / malignant Tumor / activation energy / macro CK / 活性科エネルギ- / クレアチンキナーゼ(CK) / ミトコンドリアCK / CKアイソザイム |
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| Research Abstract |
Three isoenzymes of creatine kinase(CK) are observed in the sera with malignant tumors. One is CK-BB, another is CK-linked immunoglobulin, and the other is the CK having the similar properties to mitochondrial CK(m-CK). In this studies, the physico-chemical properties of the CK obtained from the mitochondrial fraction and in the sera with malignant tumor were examined.
CK was isolated from the mitochondrial fraction obtained from bovine brain by ultracentrifugal separation. Thin-layer gel filtration and Sephacryl S-200 column chromatography revealed that the CK had a high molecular weight. The activation energy of the CK was 77.8-99.5 kJ/mol. The residual CK activity after heat treatment was 3.8-7.4%.
Atypical CK isoenzymes, CK-BB and the CK similar to m-CK were observed in the sera of patients with malignant tumor. The CK eas detected in 46 of 276 sera from malignant tumors, 178 of gastraintestinal tract, 27 of liver, 52 of breast, and 19 of lung. The CK was observed in a high rate in adenoma, but lower in scquamous cell carcinoma. The CK was not detected in the early stages of colo-rectal caricinoma(stage I,II,III), but detected stage IV.
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