| Project/Area Number |
62580046
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
家政学
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| Research Institution | Ochanomizu University |
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Principal Investigator |
ARAKAWA Nobuhiko Ochanomizu University, 家政学部, 教授 (40017223)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Emiko Ochanomizu University, 家政学部, 助手 (80154524)
OTSUKA Megumi Ochanomizu University, 家政学部, 講師 (20175243)
倉田 忠男 お茶の水女子大学, 生活環境センター, 教授 (60011920)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Food Restriction / Biorhythm / Calcium Activated Protease / カルシウム依存性プロテアーゼ / カルシウム依存性プロテアーゼインヒビター / カテプシンB / カテプシンL |
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| Research Abstract |
Effect of food restriction on biorhythm was studied. Calcium activated protease(CAF) and its endogeneous inhibitor(CAF inhibitor) activities were used as the indexes of biorhythm changes under stavation, the maximum restriction of food. The changes in calcium sensitivity of CAF in response to starvation were also studied using crude CAF preparations obtained from the skeletal muscle of rars. Total CAF activity in longissimus muscle of back of starved rats significantly increased in comparison with that of fed rats (p<0.01). On the other hand, CAF inhibitor activity had little changed during experimental period. It is therefore suggested that CAF might take an important role in myofibrillar protein degradation in response to starvation. Crude CAF activity was hardly detected in the calcium concentration less than 1 mM in the control group and in the 1 day-starved group rats, however CAF preparation was sufficiently activated in calcium less than 1mM in the groups starved for more 2 days. These results indicate the possibility of the transformation of high calcium required form of CAF (H-CAF) into low calcium required form of CAF (L-CAF) or the specific induction of L-CAF in the living cells during starvation.
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