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The Combined Effects of Vital (or Bio) Substances and Nitroso Compounds on Mutagenicity in Vivo

Research Project

Project/Area Number 62580066
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 家政学
Research InstitutionMUKOGAWA WOMEN'S UNIVERSITY

Principal Investigator

TAIRA Masahiko  Mukogawa Women's University, Home Economics, Associate Professor, 家政学部, 助教授 (00085242)

Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsNitroso compounds / Natural micronucleus frequency / Nitrosamine / Mutagenicity of MNNG / SCE test / Blood free radical density / Micro-nucleus test / Electronic spin resonance / 末梢血を用いる小核試験 / ESR(電子スピン共鳴) / MNNGの変異原生
Research Abstract

Nitroso compounds form easily under Physiological conditions, but are not so carcinogenic as might be expected from the amount formed. Based on the assumption that a factor must exist that modifies the carcinogenicity of these compounds, this study was made.
Through this study , TA1535 , the most sensitive strain of the model compound MNNG, not requiring metabolic activation, was found; the detection density threshold of 1 ug/p was determined.
This study produced a new finding; in the presence of toxic elements such as cadmium and mercury, or even non-toxic elements such as calcium and magnesium, the mutagenicity of MNNG is multiplied at a density level equal to or below the threshold value.
A micro-nucleus test was established as a system allowing continuous tracing of in vivo mutagenicity in the same animal.
Then a study was made using DMN (dimethylnitrosanine) model compounds Food-derived amine and nitrite, dimethylamine and nitrite, vitamin C, NaCl, and DMN-pasitive control, and normal food-control were administered to mice for 90 days ; changes in body weight, compound intake level, water intake volume, and feed intake level were measured every week. After autopsy , body organ weight ratio, blood free radical density, and blood peroxide density were measured. In vivo mutagenicity was continually traced by micro-nucleus testing of peripheral blood at 30-day intervals, but due to the great intraclass variation, analysis was difficult.
Nitroso reaction of intestinal flora is necessary for nitrosamines to be formed under neutral or weak alkaline conditions like those in the intestines. Little is known about the role of intestinal flora. It will be necessary to clarify that role, while at the same time analyzing the degree of intervention of nitrosamines in colon cancer, breast cancer, and other human cancer.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report

Research Products

(18 results)

All Other

All Publications (18 results)

  • [Publications] 平良昌彦: "末梢血による小核試験" 武庫川女子大学紀要. 35. 7-11 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 平良昌彦: "カドミウムのMNNGの変異原性に対する相乗作用" 日本衛生学雑誌. 43. 125 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 平良昌彦: "末梢血による小核試験(2)" 日本公衆衛生雑誌. 35. 489 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 平良昌彦: "生活関連物質の変異原性について(10)ニトロソ化反応を修飾する因子" 日本公衆衛生雑誌. 36. 851 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 平良昌彦: "腸内フロ-ラによる変異原性関連物質の生成と分解" 日本衛生学雑誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Masahiko Taira: "Micronucleus Test in Periphral Blood" Bulletin of Mukogawa Women's University, Vol.35, 373-377 (1987).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Masahiko Taira: "Synergistic mutagenic effects of cadmium plus MNNG in Salmonella typhimurium" Japanese Journal of Hygine Vol.43, 125 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Masahiko Taira: "Synthesis and Decomposition of fecal mutagens by intestinal microflora" Japanese Journal of Hygine Vol.45, in press (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 平良昌彦: "末梢血による小核試験" 武庫川女子大学紀要. 35. 7-11 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 平良昌彦: "カドミウムのMNNGの変異原性に対する相乗作用" 日本衛生学雑誌. 43. 125 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 平良昌彦: "末梢血による小核試験(2)" 日本公衆衛生雑誌. 35. 489 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 平良昌彦: "生活関連物質の変異原性について(10)ニトロソ化反応を修飾する因子" 日本公衆衛生雑誌. 36. 851 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 平良昌彦: "腸内フロ-ラによる変異原性関連物質の生成と分解" 日本衛生学雑誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] 平良昌彦: 日本衛生学雑誌. 43. 125 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 平良昌彦: 日本公衆衛生雑誌. 35. 489 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 平良昌彦: 武庫川女子大学紀要. 35. 373-377 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 平良 昌彦: 日本衛生学雑誌. 43. 125 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 平良 昌彦: 武庫川女子大学院紀要. 35. (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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