• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Sugar chains of lysosomal enzymes and sphingolipid activator proteins, and the relationship with their transport mechanism

Research Project

Project/Area Number 62580123
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionKobe University

Principal Investigator

YAMASHITA Katsuko  Kobe University School of Medicine, Associate Professor, 医学部, 助教授 (70030905)

Co-Investigator(Kenkyū-buntansha) KOIDE Norio  Okayama University School of Medicine, Assistant Professor, 医学部, 講師 (20142333)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsLysosomal enzyme / Sphingolipid activator protein / Glycoprotein / Traffic signal / Lectin / レクチン / 糖脂質活性化因子 / 細胞内輸送シグナル / リソソーム局在性糖蛋白質 / 糖鎖の極微量分析
Research Abstract

The enzymatic degradation of a number of sphingolipids in the lysosomes is stimulated by small (8 to 13 kD) glycoproteins named sphingolipid activator proteins SAP-1, SAP-2, and SAP-3. Since these activators are translocated into lysosomes after biosynthesis, their asparagine-linked sugar chains should act as a traffic signal to lysosomes.
In order to elucidate the traffic mechanism of SAPs to the lysosome and the role of acid glycosidases in lysosomal degradation of sugar chains of glycoproteins, we have investigated the structures of sugar chains released from SAR-1 purified from normal human liver and GM1 gangliosidosis, Type 1, liver, guinea pig kidnney SAP-3, and Gaucher disease spleen SAP-2. The sugar chains of SAPs except SAP-1 purified from GM1 gangliosidosis liver were equal qualitatively with one another and their structures were the following eight components: Man 1 6(Man 1 3)Man 1 4GlcNAc 1 4( Fuc 1 6)GlcNAc, Man 1 6Man 1 4GlcNAc 1 4( Fuc 1 6) GlcNAc, Man 1 4Man 1 4GlcNAc 1 4( Fuc 1 6)GlcNAc, GlcNAc 1 4GlcNAc, and GlcNAc, indicating that sugar chains of native SAPs were degraded sequencially by exoglycosidases in lysosoms. It seems difficult to speculate the role in the transport mechanism of those suger chains of the native protein like SAP-1 purified from the normal organs.
While,fifteen percent of the sugar chains of SAP-1 purified from liver of GM1 gangliosi-dosis, Type 1, contained sialylated mono- to tetra-antennary oligosaccharides and the remaining 85 % was neutral mono-, bi-, tri-, and tetra- antennary complex type sugar chains containing fucosylated and nonfucosylated trimannosyl cores, and N-acetyllactosamine groups at outer chains, although they are slightly degraded. These results indicate that some complex type sugar chains including N-acetyllactosamine group, the fucosyl residue linked to the proximal N-acetylglucosamine, and the sialic acid et al. may act as a traffic signal to transport SAP-1 from the golgi apparatus to the lysosome.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Yamashita,K.: Journal of Biological Chemistry. 262. 1602-1607 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita,K.: Biochemistry. 27. 5565-5573 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita,K.: Journal of Biological Chemistry. 263. 17482-17489 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita,K.: Journal of Biological Chemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita,K.: Journal of Biochemistry(Tokyo).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita,K.: Submitted for Publication.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita,K.: "Allomyrina dichotoam Lectins in Methods in Enzymology" Academic Press, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kobata,A.: "Extention and Limit of Affinity Chromatography of Oligosaccharides on an E_4-PHA-agarose Column,in Methods in Enzymology" Academic Press, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita, K.: "Carbohydrate Binding Properties of Complex-type Oligosaccharides on Immobilized Datura stramonium Lectin." Journal of Biological Chemistry. 262. 1602-1607 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita, K.: "Comparative Studies of the Sugar Chains of Aminopeptidase N and Dipeptidylpeptidase IV Purified from Rat Kidney Brush-Border Membrane." Biochemistry. 27. 5565-5573 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita, K.: "Carbohydrate Binding Specificity of Immobilized Allomyrina dichotoma Lectin II." Journal of Biological Chemistry. 263. 17482-17489 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita, K.: "Altered Glycosylation of Serum Transferrin of Patients with Hepatocellular Carcinoma." Journal of Biological Chemistry.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita, K.: "Comparative Study of Sugar Chains of -Glutamyltranspeptidase Purified from Human Hepatocellular Carcinoma and from Human Liver." Journal of Biochemistry (Tokyo).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita, K.: "Characteristics of Asparagine-linked Sugar Chains of Sphingolipid Activator Protein 1 Purified from Normal Human Liver and GM_1" gangliosidosis, Type 1, Liver.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita, K.: Allomyrina dichotoma Lectins in Methods in Enzymology. Academic Press, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kobata, A.: Extention and Limit of Affinity Chromatography of Oligosaccharides on an E_1-PHA-agarose Column. in Methods in Enzymology. Academic Press, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita,K.: Biochemistry. 27. 5565-5573 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamashita,K.: Journal of Biological Chemistry. 263. 17482-17489 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamashita,K.: Journal of Biological Chemistry.

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamashita,K.: Journal of Biochemistry(Tokyo). in press.

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamashita,K.: "Allomyrina dichotoma Lectins in Methods in Enzymology" Academic Press, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kobata,A.: "Extention and Limit of Affinity Chromatography of Oligosaccharides on an E_4-PHA-agarose Column.in Methods is Enzymolgy" Acadenic Press, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] A. Hitoi: Journal of Biochemistry(Tokyo). 100. 29-41 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] K. Yamashita: Journal of Biological Chenistry. 262. 1602-1607 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] K. Yamashita: Cancer Research. 47. 3451-3459 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] K. Yamashita: Biochemistry.

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1987-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi