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Structural studies on Functional Abnormality of Factor XII and Fibrinogen.

Research Project

Project/Area Number 62580126
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionKyushu University

Principal Investigator

ITO Akio (1988)  Faculty of Science, Kyushu University, Associate Professor, 理学部, 助教授 (30037379)

宮田 敏行 (1987)  九州大学, 理学部, 助手 (90183970)

Co-Investigator(Kenkyū-buntansha) KAWABATA Shun-ichiro  Faculty of Science, Kyushu University, Research Associate, 理学部, 助手 (90183037)
SAITO Hidehiko  Faculty of Medicine, Nagoya University, Professor, 医学部, 教授 (20153819)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsFibrinogen Nagoya / Fibrin Monomer / Molecular Defect / Fibrinogen -chain / Factor IX Niigata / Serine Protease / トリプシンペプチド地図 / 分子異常XII因子 / カリクレイン / XII因子Washington D.C. / アミノ酸配列 / フィブリノーゲン / XII因子 / 血液凝固因子 / 分子異常症 / 遺伝病
Research Abstract

(1) Fibrinogen Nagoya, a Replacement of Glutamine-329 by Arginine in the -chain. That Impairs the Polymerization of Fibrin Monomer: Structural studies on a hereditary abnormal fibrinogen, fibrinogen Nagoya were performed to identify the abnormality responsible for the impaired polymerization of fibrin nomomer. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. fibrinogen Nagoya showed the presence of an extra protein band with an apparent molecular weight of 49,500 in addition to the normal three subunit chains. Amino acid sequence analysis of a peptide isolated from a lysyl endopeptidase digest of one of the cnbr fragments derived from fibrinogen nagoya indicated that Gln-329 in the -chain had been replaced by Arg. This substitution can be explained by a single uncleotide chainge in the dodon for Gln-329 (CAG - CGG). We conclude that GLN-329 in the -chain is indispensable for the normal polymerization of fibrin monomer.
(2) Blood Clontting Factor IX Niigata: Substitution of Alanine-390 by Valine in the Catalytic Domain: Factor IX Niigata is s mutant factor IX responsible for the moderately severe hemophilia B in a patient who has a normal level of factor IX antigen with reduced clotting activity (1-4% of normal). We reported previously that the purified mutant protein could be converted to the factor IXa form by factor XIa/Ca^<2+> at a rate similar to that in the case of normal factor IX, but the resulting mutant factor IXa could not activate factor X in the presence of factor XIII, Ca^<2+>, and phospholipids. In the present study, we analyzed factor IX Niigata at the structural level to elucidate the molecular abnormality responsible for the loss of clotting activity. Amino acid sequence analysis of a peptide obtained on lysyl endopeptidase digestion, coupled with subsequent SP-V8 digestion, demonstrated that the alanine at position 390 was substituted by valine in the catalytic domain of the factor IX Niigata molecule.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Miyata,T.: Biochemistry. 26. 1117-1122 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyata,T.: J.Biochem.102. 93-101 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Muta,T.: J.Biochem.101. 1321-1330 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyata.T.: J.Biochem.105. 730-734 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sugimoto,M.: J.Biochem.104. 878-880 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takeya,H.: J.Biol.Chem.263. 14868-14877 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 宮田敏行 他: "続生化学実験講座6章 自動エドマン分解" (株)化学同人, 20 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 宮田敏行 他: "蛋白質・核酸・酵素 33巻No.5" (株)共立出版, 5 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyata, T. et al.: "Prothrombin Tokushima: A Replacement of Arginine-418 by Tryptophan that Impairs the Fibrinogen Clotting Activity of Derived Thrombin Tokushima." Biochemistry. 26. 1117-1122 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyata, T. et al.: "Fibrinogen Kawaguchi and Osaka: An Amino Acid Substitution of A Arginine-16 to Cysteine which forms an Extra Interchain Disulfide Bridge between the Two A Chains." J. Biochem.102. 93-101 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyata, et al.: "Fibrinogen Nagoya, a Replacement of Glutamine-329 by Arginine in the -Chain that Impairs the polymerization of FIbrin Monomer." J. Biochem.105. 730-734 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sugimoto, M. et al.: "Blood Clotting Factor IX Niigata: An Amino Acid Substitution of Alanine-329 by Valine in the Catalytic Domain." J. Biochem.104. 878-880 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takeya, H. et al.: "Bovine Factor VII: Its Purification and Complete AMino Acid Sequence." J. Biol. Chem.263. 14868-14877 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyata, T:; Iwanaga, S.: Dysfibrinogenemia and Dysprothombinemia (Protein Nucleic Acid Enzyme, 33, No.5).Kyoritsu Shuppan,959-963 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyata,T.: J.Biochem.105. 730-734 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sugimoto,M.: J.Biochem.104. 878-880 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takeya,H.: J.Biol.Chem.263. 14868-14877 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tokunaga,F.: J.Biol.Chem.263. 14741-17481 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nakamura,T.: J.Biol.Chem.263. 16709-16713 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hase,S.: J.Biochem.104. 867-868 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 宮田敏行 他: "蛋白質・核酸・酵素33巻,NO.5" (株)共立出版, 959-963 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 宮田敏行: "日本血液学会雑誌51巻,NO.7(ミニレヴュー)" 社団法人日本血液学会, 1285-1288 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T. Miyata: Biochemistry. 26. 1117-1122 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] T. Miyata: J. Biochem.102. 93-101 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] F. Tokunaga: Eur. J. Biochem.167. 405-416 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] S. Kaida: J. Biochem.102. 1177-1186 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] T. Sueyoshi: J. Biol. Chem.262. 2768-2779 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] T. Miyata: J. Biol. Chem.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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