| Project/Area Number |
62580137
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
ITOH Nobuyuki Kyoto Sangyo University, Associated professor, 国土利用開発研究所, 助教授 (10110610)
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| Co-Investigator(Kenkyū-buntansha) |
NAKADA Hiroshi Kyoto Sangyo University, Associated professor (90113141)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Choline acetyltransferase / Drosophila / Gene / 塩基配列 / ドロソフィラ |
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| Research Abstract |
Choline acetyltransferase is the biosynthetic enzyme for the neurotransmitter acetylcholine. By screening Drosophila cDNA library with a mixture of three different monoclonal antibodies to Drosophila choline acetyltransferase, we isolated drosophila choline acetyltransferase cDNA. The cDNA insert consisted of a coding region 2,190 nucleotides long and a3' noncoding region 284 nucleotides long. Northern analysis of Drosophila head mRNA with the cDNA insert as a probe showed the choline acctyltransferase mRNA to be approximately 4,700 nucleotides long. We compared the amino acid sequences of Drosophila choline acetyltransferase and Torpedo acetylcholinesterase and found a global homology of 21-44% identity between these proteins. Six homologous segments of 40-60 amino acids cover 38% and 54% of the sequences, raising the possibility of a common evolutionary origin. We also isolated and characterized thd cDNA related with Drosophila choline acetyltransferase.
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