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Inhibition of (Na,K)ATPase by the extract from Artemia salina

Research Project

Project/Area Number 62580159
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 代謝生物化学
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

KAWAMURA Masaru  University of Occupational and Environmental Health, 医学部, 教授 (00049066)

Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1988: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords(Na,K)ATPase / Inhibitor / Artemina salina / Fatty acid / りん脂質 / (Na, K)ATPase / アルテミア / インヒビター
Research Abstract

Measurement of levels of (Na,K) ATPase subunit in developing Artemia salinahas indicated that both alpha and beta subunit are present in the embryos of this organism in the early stage of development. Nevertheless, Artemia in these stages does not show any detectable (Na,K)ATPase activity, suggesting that the presence of inhibitors for (Na,K)ATPase in developing Artemia. In this study, the endogenous inhibitors were isolated from Artemia and characterized as follows;
1. Digestion of the inhibitors with trypsin or papain did not have any significant effect of their inhibitory activity. Furthermore heat treatment did not affect the inhibitory activity.
2. The inhibitors extracted with organic solvent yielded two active spots on TLC plates, both containing longchain fatty acids. These results suggested that the inhibitory activity found in Artemia were due to non-esterified long chain fatty acids and their esters. In order to examine the effect of fatty acids on (Na,K)ATPase, the enzyme was purified from Artemia and delipidated with a detergent, CHAPS.
3. Upon delipidation, the activity was although 10-25 phospholipids were still left on an enzyme.
4. With the addition of exogeneous phospholipids, the activity was restored. The enzyme delipidated in the presence of KCl reassociated phospholipids more firmly than the enzyme delipidated in the presence of NaCl.
The delipidated enzyme would be a good tool for studying the action of fatty acids on (Na,K)ATPase

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 朝倉淳: 産業医大雑誌. 9(1). 29-33 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Noguchi,S.: FEBS Lett.225(1,2). 27-32 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takeda,K.: J.UOEH,. 10. 59-62 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Morohashi,M.: J.Biochem.103. 1073-1077 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takeda,K.: FEBS Lett.238. 201-204 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Noguhci,S.: Biochem.Biopys.Res.Commun.155. 1237-1243 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kawakami,K: "Perspectives of Biological Energy Trunsduction" Academic Press, 257-263 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kawamura,M.: "Progr.in Clin.and Biol.Res." Alan R.Liss,Inc., 93-98 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Morohashi, M.: "Idenrification of (Na,K)ATPase inhibitor in developing Artemia as long chain fatty acids"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Morohashi, M.: "Effect of Na^+ and K^+ on Artemia salina (Na,K)ATPase solubilized with a zwitterionic detergent, CHAPS" J.Biochem.103. 1073-1077 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takeda, K.: "Functional Activity of oligosaccharide deficient (Na,K)ATPase expressed in Xenopus oocytes" FEBS Lett.238. 201-204 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Noguchi, S.: "Ouabain sensitivity of a chimeric alpha subunit (Torpedo/Rat) of the (Na,K)ATPase expressed in Xenopus oocytes" Biochem.Biopys.Res.Commun.155. 1237-1243 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Noguchi, S.: "Expression of functional (Na,K)ATPase from cloned cDNAs" FEBS Lett.225. 27-32 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kawamura, M.: Structure and function of the beta subunit of (Na,K)ATPase In: Progr.in Clin.and Biol.Res.Alan R. Liss, Inc., 93-98 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takeda,K.: J.UOEH,. 10. 59-62 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Morohashi,M.: J.Biochem.103. 1073-1077 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takeda,K.: FEBS Lett.283. 201-204 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Noguchi,S.: Biochem.Biopys.Res.Commun.155. 1237-1243 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohta,T.: "Progr.in Clin.and Biol.Res." Alan R.Liss,Inc., 107-114 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kawamura,M.: "Progr.in Clin.and Biol.Res." Alan R.Liss.Inc., 93-98 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 朝倉 淳: 産業医科大学雑誌. 9. 29-33 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] S.Noguchi: FEBS Lett.225. 27-32 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] K.Takeda: J.UOEH. 10. 59-62 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] M.Kawamura: In:(Na/K)ATPase Eol A.B.Maunsbach A.R.Liss Inc.N.Y.(1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] M.Morohashi: J.Biochem.(1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] K.Takeda: J.Biol.Chem.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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