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Studies on the NADPH O^-_-generating oxidase in neutrophils

Research Project

Project/Area Number 62580161
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 代謝生物化学
Research InstitutionThe Tokyo Metropolitan Institute of Medical Science(TIMS)

Principal Investigator

KAKINUMA Katsuko  TIMS, Department of Inflammation Research, 炎症研究部, 室長 (30109946)

Co-Investigator(Kenkyū-buntansha) 田中 昭子  財団法人東京都臨床医学総合研究所, 炎症研究部, 研究員 (80274314)
TSUNAWAKI S.  TIMS, Department of Inflammation Research, 炎症研究部, 研究員 (00211384)
KANEDA M.  TIMS, Department of Inflammation Research, 炎症研究部, 研究員 (50113494)
FUJII H.  TIMS, Department of Inflammation Research, 炎症研究部, 研究員 (70209013)
CHIBA T.  TIMS, Department of Inflammation Research, 炎症研究部, 研究員 (20188486)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1988: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1987: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsNADPH oxidase / O^-_-generating enzyme / O_2^-生成酵素 / チトクロムb558 / フラビン酵素 / O<-( / )2>生成酵素 / チトクロムb_<558> / 活性酸素生成酵素 / NADPH oxidase / FAD酵素
Research Abstract

The NADPH (O^-_-generating) oxidase of neutrophils has been thought to be a bransmembrane-electron transport system, with its redox centers such as a b-type cytochrome and a flavin enzyme. We prepared the membrane oxidase from either stimulated or resting neutrophils of pig blood and separated a flavin enzyme and a cytochrome b_<558> fraction from the solubilized oxidase by a single step of preparative isoelectric focussing to avoid denaturation of the redox centers. 1) the flavin enzyme focussed at pI 5.0 was mainly of about 70 kDa molecular mass. The Km value for oxygen was in the range of 72-134 muM, much higher than that of the membrane-oxidase (6muM). 2) The aim of our ESR studies was to characterize a b-type cytochrome, so called "cytochrome b_<558>", located in the NADPH oxidase system in neutrophil membranes. The cytochrome b_<558> fraction revealed severl resonance signls; a low spin signal(s) at g=3.52 (3.23), and non-heme metal signals around g=2 region. The present results of the heme in cytochrome b_<558> are in accord with the absorption spectrum and its insensitivity to KCN and CO, characteristic for a low-spin state of the iron in the heme.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] K.KAKINUMA,et.al.: J.Biol.Chem.262. 12316-12322 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.KAKINUMA,;T.Chiba.;H.Fujii: Elsevier publiching Co.Medical,Biochem.Chem.Aspect of Free Radicals.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H.Fujii.;K.KAKINUMA: Biochem.Biophys.Acta,.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.Fukuhara,;Y.Ise.;K.KAKINUMA: Febs.Lelt.229. 150-156 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Yamaguchi,;T.Hayakawa.;M.Kaneda.;K.KAKINUMA: J.Biol.Chem.264. 112-118 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 綱脇祥子、柿沼カツ子、倉辻忠俊: 炎症(炎症学会誌). 9. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 柿沼カツ子、山村雄、吉利和監修: "「新医科学大系」白血球と感染防御" 中山書店, 191-216 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 柿沼カツ子、三輪谷俊夫監修: "「医学細菌学」4巻、白血球の感染防御" 菜根出版, 231-270 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Kakinuma; Y. Fukuhara; M. Kaneda: "The Respiratory Burst Oxidase of neutrophils-separation of an FAD enzyme and its Characterization" J. Biol. Chem.262. 12316-12322 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y. Fukuhara; Y. Ise; K. Kakinuma: "Immunological Studies on the Respiratory Burst Oxidase of Pig Blood Neutrophils" FEBS. LETT.229. 150-156 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. Yamaguchi; T. Hayakawa; M. Kaneda; K. Kakinuma; T. Yoshikawa: J. Biol. Chem.264. 112-118 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Kakinuma; T. Chiba; H. Fujii: "The Respiratory Burst Oxidase of Neutrophils - Further Studies on the Flavin enzyme (p15.0)." ESR and Free Radicals (Japan ESR Society). 121-124 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H. Fujii; K. Kakinuma: "Direct measurement of superoxide anions produced by Biological Systems by Electron Spin Resonance", in "Biochemical andChemical Aspects of Free Radicals.Publishers Elsevier Pub. Co., (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Kakinuma; T. Chiba; H. Fujii: "Respiratory Burst Oxidase of Neutrophils", in "Biochemical and Chemical Aspects of Free Radicals". Elsevicr Publishing Co., (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.KAKINUMA,et.al.: J.Biol Chem.262. 12316-12322 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.KAKINUMA,;T.Chiba;H.Fujii.: Elsevier publiching Co.Medical,Biochem.Chem.Aspect of Free Radicals.

    • Related Report
      1988 Annual Research Report
  • [Publications] H.Fujii,;K.KAKINUMA.: Biochim.Biophys.Acta,.

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.Fukuhara,;Y.Ise.;K.KAKINUMA.: Febs.Lett.229. 150-156 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Yamaguchi,;T.Hayakawa,;M.Kaneda,;K.KAKINUMA.: J.Biol.Chem.264. 112-118 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 網脇祥子、柿沼カツ子、倉辻忠俊: 炎症 (炎症学会誌). 9. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 柿沼カツ子: "「新医科学大系」白血球と感染防御" 中山書店, 191-216 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 柿沼カツ子: "「医学細菌学」4巻、白血球の感染防御" 菜根出版, 231-270 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] K. Kakinuma: J. Biol. Chem.262. 12316-12322 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] A. Hata-Tanaka: FEBS Lett.214. 279-284 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] T. Chiba: Biochim. Biophys. Acta. 930. 10-18 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 千葉 司: 災症. 7. 517-521 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 柿沼 カツ子: "ファゴサイトーシス,『新生理学大系』(勝木保治,内園耕二 監修),第3巻 膜輸送の生理学(星猛,香川靖雄 編)" 医学書院, 9 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 柿沼 カツ子: "29・2・3 過酸化水素生成の測定 29・3糖代謝の変化,32・1 NADPIIオキシダーゼ活性の測定,『続生化学実験講座』第8巻 血液(下)(日本生化学会)" 東京化学同人, 17 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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