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Induction of numerical chromosome changes and neoplastic transformation by radiations.

Research Project

Project/Area Number 62580164
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 放射線5生物学
Research InstitutionKanazawa University

Principal Investigator

SUZUKI Fumio  Faculty of Pharmaceutical Sciences, Kanazawa University, 薬学部, 助教授 (10019672)

Co-Investigator(Kenkyū-buntansha) MATSUNAGA Tsukasa  Faculty of Pharmaceutical Sciences, Kanazawa University, 薬学部, 教務職員 (60192340)
NIKAIDO Osamu  Faculty of Pharmaceutical Sciences, Kanazawa University, 薬学部, 教授 (60019669)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1988: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsX-rays / Ultraviolet light / Golden hamster / Neoplastic transformation / Trisomy / Oncogene / トランスフェクション / 放射線 / 染色体変化
Research Abstract

Using gloden hamster embryo (GHE) cells, we examined the induction of chromosome changes and neoplastic transformation by X-rays or ultraviolet litht (UV).
Unirradiated cells were grown at a constant growth rate for 7 passages but later ceased to divide. X-ray- or UV-irradiated cells escaped from senescence ans showed morphological transformation accompanying with the expression of the ability to form colonies in soft agar medium at later passages. In contrast to these changes, the aquisition of tumorigenicity in nude mice was observed much later (11 passages after the irradiation). When methaphese chromosomes were prepared at various passages after X-irradiation, one or two additional chromosomes were detected in the cells depending on passage numbers. For example, almost all the cells showing 45 chromosomes at passage 5 contained trisomy of chromosome 7 and the cells transferred for 13 passages had futher numerical chromosome changes on chromosome 6,9 and/or 20. Similar chromosome changes were observed in anchorage-independent and tumorigenic cells induced by UV. Mouse NIH3T3 cells transfected with DNA from various transformed GHE cells expressed malignant phenotypes. From Southern blot analysis, all malignant NIH3T3 cells derived from tumors after transfection were found to contain some amount of golden hamster DNA.
These results suggest that radiation induced transformation is a multistep in GHE cells, and that the numerical change of specific chromosomes and the activation of some oncogenes may play an important role for the expression of each transformed phenotypes.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Ehud Ben-Hur: Photochemistry and Photobiology. 45. 227-230 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 鈴木文男: 放射線科学. 31. 39-43 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Toshio Mori: Mutation Research. 194. 263-270 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Keiji Suzuki: Cancer Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ehud Ben-Hur; Tetsuo Fujihara; Fumio Suzuki; Mortimer M. Elkind: "Genetic toxicology of the photosensitization of Chinese hamster cells by phthalocyanines." Photochemistry and Photobiology. 45. 227-230 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fumio Suzuki: "Radiation and cell transformation: The meaning of multistep nature." Radiological Sciences. 31. 39-43 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Toshio Mori; Tsukasa Matsunaga; Tohru Hirose; Osamu Nikaido: "Establishment of a monoclonal antibody recognizing ultraviolet light-induced (6-4)photoproducts." Mutation Research. 194. 263-270 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Keiji Suzuki; Fumio Suzuki; Masami Watanabe; Osamu Nikaido: "Multistep nature of X-ray-induced neoplastic transformation in golden hamster embryo cells: Expression of transformed phenotypes and stepwised changes in karyotypes." in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Toshio,Mori: Mutation Research. 194. 263-270 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Keiji,Suzuki: Cancer Research.

    • Related Report
      1988 Annual Research Report
  • [Publications] 鈴木文男: 放射線科学. 31. 39-43 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] K. Suzuki;F. Suzuki;M. Watanabe;O. Nikaido: Submitted to Cancer Res.,.

    • Related Report
      1987 Annual Research Report
  • [Publications] K. Suzuki;F. Suzuki;M. Watanabe;O. Nikaido: Submitted to Cancer Res.,.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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