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Construction of a new centrifuge microscope and its application on biological sciences

Research Project

Project/Area Number 62840021
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionHITOTSUBASHI UNIVERSITY

Principal Investigator

KAMITSUBO Eiji  Hitotsubashi Univ., Dept. Comm., Prof., 商学部, 教授 (60111697)

Co-Investigator(Kenkyū-buntansha) KIKUYAMA Munehiro  The Univ. of the Air, Assoc. Prof., 助教授 (90131010)
YOSHIMOTO Yasuaki  Kansai Medical School, Prof., 教養部, 教授 (30124225)
HIRAMOTO Yukio  The Univ. of the Air, Prof., 教授 (50011440)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1987: ¥8,000,000 (Direct Cost: ¥8,000,000)
Keywordscentrifuge microscope / cytoplasmic streaming / echinoderm egg / plasmodial cytoplasmic streaming / characean cell / Vallisneria / 原形質流動 / 非接触電気刺激法 / ストロギ型遠心顕微鏡 / フラスモ節間細胞 / 皮層下アクチン繊維束 / 遠心加速度 / 高性能遠心顕微鏡 / ストロボスコープ / 可変リアルタイム微分処理機能 / 電子回転計
Research Abstract

We constructed a new centrifuge microscope of the stroboscopic type(CMS-11). The CMS-11 is composed of a video-enhanced contrast microscope combined with a stroboscopic light source, a mechanism for centrifugation of a specimen, and a control panel for triggering the light source synchlonously with the rotation of the specimen [for detail see Kamitsubo et al Protoplasma (1989) 152:148-155].
Using the new CMS-11 the cyto lasmic streaming in characean internodal cells, was studied (Kamitsubo et al 1989). Plasmodial cytoplasmic streaming under centrifugal acceleration was observed with the CMS-11 (Kamitsubo 1989). The mechanical structure of the cytoplasm of the echinoderm egg was determined by "Gold particle method" using centrifuge microscope (Kaneda et al 1990). Using the CMS-11, effects. of light irradiation on the passive movement of chloroplasts in dark-adapted Vallisneria mesophyll cells were examined (Takagi et al 1989).

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Kamitsubo,E.,M.Kikuyama & I.Kaneda: "Apparent viscosity of endoplasm of characean internodal cell measured by centrifuge method" Protoplasma. Suppl 1. 10-14 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kamitsubo,E: "Plasmodial cvtoplasmic streaming under centrifugal acceleration observed with a new centrifuge microscope" J.Muscle Res Cell Motility. 10. 269 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 菊山宗弘,上坪栄治: "原形質流動の研究における遠心処理の適用" 生体の科学. 39. 500-501 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takagi,S.,E.Kamitsubo & R.Nagai: "Light action on chloroplast behavior under centrifugal acceleration" Plant water relations and growth under stress.Proceedings of the Yamada Conference XX11(Osaka). 455-457 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kamitsubo,E.,Y.Ohashi & M.Kikuyama: "Cytoplasmic streaming in internodal cells of Nitella under centrifugal acceleration:a study done with a newly constructed centrifuge microscope" Protoplasma. 152. 148-155 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kaneda,I.,E.Kamitsubo & Y.Hiramoto: "The mechanical structure of the cytoplasm of the echinoderm egg determined by“Gold particle method"using a centrifuge microscope" Develop.Growth & Differ.32. 15-22 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hiramoto,Y.: "Mechanics of dividing cells.In“Progress and new directions of biomechanics(Eds.Y.C.Fung et al)" Mita Press,Tokyo, 444 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kamitsubo, E., M.Kikuyama & I.Kaneda: "Apparent viscosity of endoplasm of characean internodal cell measured by centrifuge method" Protoplasma. Suppl 1. 10-14 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kamitsubo, E.: "Plasmodial cytoplasmic streaming under centrifugal acceleration observed with a new centrifuge microscope" J. Muscl Res. Cell Motility. 10. 269-270 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takagi, S., E.Kamitsubo & R.Nagai: "Light action on chloropast behavior under centrifugal acceleration" Plant water relations and growth under stress. Proceedings of the Yamada Conference XX11 (Osaka). 455-457 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kamitsubo, E.Y.Ohashi & M.Kikuyama: "Cytoplasmic streaming in internodal cells of Nitella under centrifugal acceleration: a study done with a newly constructed centrifuge microscope" Protoplasma. 152. 148-155 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kaneda, I., E.Kamitsubo & Y.Hiramoto: "The mechanical structure of the cytoplasm of the echinoderm egg determined by "Gold particle method" using a centrifuge microscope" Develop. Growth & Differ. 32. 15-22 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hiramoto, Y.: Mechanics of dividing cells, in "Progress and new directions of biomechanics (Eds. Fung, Y.C. et al). Mita Press, Tokyo, 3-12 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kamitsubo,E.,M.Kikuyama&I.Kaneda: "Apparent viscosity of endoplasm of characean internodal cell measured by centrifuge method" Protoplasma. Suppl 1. 10-14 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kamitsubo,E.: "Plasmodial cvtoplasmic streaming under centrifugal acceleration observed with a new centrifuge microscope" J.Muscle Res Cell Motility. 10. 269 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 菊山宗弘,上坪英治: "原形質流動の研究における遠心処理の適用" 生体の科学. 39. 500-501 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takagi,S.,E.Kamitsubo&R.Nagai: "Light action on chloroplast behavior under centrifugal acceleration" Plant water relations and growth under stress.Proceedings of the Yamada Conference XXII(Osaka). 455-457 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kamitsubo,E.,Y.Ohashi&M.Kikuyama: "Cytoplasmic streeaming in internodal cells of Nitella under centrifugal acceleration:a study done with a newly constructed centrifuge microscope" Protoplasma. 152. 148-155 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kaneda,I.,E.Kamitsubo&Y.Hiramoto: "The mechanical structure of the cytoplasm of the echinoderm egg determined by“Gold particle method"using a centrifuge microscope" Develop.Growth&Differ.32. 15-22 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hiramoto,Y.: "Mechanics of dividing cells.In“Progress and new directions of biomechanics(Eds.Y.C.Fung et al)" Mita Press,Tokyo, 444 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] I.Kaneda;E.Kamitsubo;Y.Hiramoto: J.Muscle Res.Cell Motility. 8. 285 (1937)

    • Related Report
      1988 Annual Research Report
  • [Publications] E.Kamitsubo;M.Kikuyama;I.Kaneda: Protoplasma. Suppl 1. 10-14 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 菊山宗弘,上坪英治: 生体の科学. 39(5). 500-501 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] I.Kaneda;E.Kamitsubo;Y.Hiramoto: Cell Motility and the cytoskeleton.

    • Related Report
      1988 Annual Research Report
  • [Publications] E.Kamitsubo;Y.Ohashi;M.Kikuyama: Protoplasma.

    • Related Report
      1988 Annual Research Report
  • [Publications] E.Kamitsubo: J.Muscle reserch and cell motility.

    • Related Report
      1988 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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