| Project/Area Number |
62840021
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理学
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| Research Institution | HITOTSUBASHI UNIVERSITY |
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Principal Investigator |
KAMITSUBO Eiji Hitotsubashi Univ., Dept. Comm., Prof., 商学部, 教授 (60111697)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUYAMA Munehiro The Univ. of the Air, Assoc. Prof., 助教授 (90131010)
YOSHIMOTO Yasuaki Kansai Medical School, Prof., 教養部, 教授 (30124225)
HIRAMOTO Yukio The Univ. of the Air, Prof., 教授 (50011440)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1987: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | centrifuge microscope / cytoplasmic streaming / echinoderm egg / plasmodial cytoplasmic streaming / characean cell / Vallisneria / 原形質流動 / 非接触電気刺激法 / ストロギ型遠心顕微鏡 / フラスモ節間細胞 / 皮層下アクチン繊維束 / 遠心加速度 / 高性能遠心顕微鏡 / ストロボスコープ / 可変リアルタイム微分処理機能 / 電子回転計 |
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| Research Abstract |
We constructed a new centrifuge microscope of the stroboscopic type(CMS-11). The CMS-11 is composed of a video-enhanced contrast microscope combined with a stroboscopic light source, a mechanism for centrifugation of a specimen, and a control panel for triggering the light source synchlonously with the rotation of the specimen [for detail see Kamitsubo et al Protoplasma (1989) 152:148-155].
Using the new CMS-11 the cyto lasmic streaming in characean internodal cells, was studied (Kamitsubo et al 1989). Plasmodial cytoplasmic streaming under centrifugal acceleration was observed with the CMS-11 (Kamitsubo 1989). The mechanical structure of the cytoplasm of the echinoderm egg was determined by "Gold particle method" using centrifuge microscope (Kaneda et al 1990). Using the CMS-11, effects. of light irradiation on the passive movement of chloroplasts in dark-adapted Vallisneria mesophyll cells were examined (Takagi et al 1989).
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