| Project/Area Number |
62850072
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
計測・制御工学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
INABA Humio Tohoku Univ. RIEC, Professor, 電気通信研究所, 教授 (90006213)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Shunichi Tohoku Univ. RIEC, Research Associate, 電気通信研究所, 助手 (30162431)
ITO Hiromasa Tohoku Univ. RIEC, Assistant Professor, 電気通信研究所, 助教授 (20006274)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥16,300,000 (Direct Cost: ¥16,300,000)
Fiscal Year 1989: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1988: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1987: ¥9,500,000 (Direct Cost: ¥9,500,000)
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| Keywords | Laser microscope / Biological cell / Cell fusion / Micro-processing / Semiconductor laser / Optical trapping / Excimer laser / Focused laser beam / レザ-顕微鏡 / レーザ光精密照射 / レーザ顕微鏡 / 圧電素子制御 |
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| Research Abstract |
This project aims the research and development of extremely precise actuator system for biotechnology and lifescience applications utilizing diffraction-limited focused laser beam to achieve non-contact micro-processing, trapping. transfer and others of a single biological cell. For this purpose, we newly developed highly precise micro-processing system using ultraviolet pulsed excimer laser,and the single beam optical trapping technique for a trapping and transfer of a single biological cell using continuous-wave laser beam. In the highly precise laser micro-processing system. anultraviolet pulsed laser beam which vas focused upto the diffraction limit by an objective is irradiated on a sample through an inverted-type fluorescence microscope system. The portion of the sample stage is controlled by piezo-electric motor with a resolution less than 1 mum. Thus, based on various experiments for biological cells using this system, we have succeed in demonstrating perforation and cutting of a part of various cells together with cell fusion by means of the laser irradiatim.
Using an Ar laser of 488 nm and semiconductor diode lasers of 0.83 and 1.33 mum, non-contact and non-destructive three-dimensional optical trapping was demonstrated for polystyrene latex spheres and several single biological cells including lymphocyte, erythrocyte, and yeast. It was proved that near-infrared laser light is more suitable for their trapping than visible Ar-laser light because the cell damage caused by laser irradiation is not observed in the case using near-infrared semiconductor diode lasers.
Hence it can be confirmed that this research and development have contributed largely to establish the basic technology for novel highly precise actuator system for single biological cell using laser light which is expected to be extended widely to bioscience and biotechnology in the near future.
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