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Evaluation of Water Treatment Processes Using Bacteriophages as Tracer

Research Project

Project/Area Number 62850101
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 都市工学・衛生工学
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

OHGAKI Shinichiro (1988-1989)  Faculty of Engineering, The University of Tokyo Professor, 工学部, 教授 (20005549)

大垣 真一郎 (1987)  東京大学, 工学部, 助教授

Co-Investigator(Kenkyū-buntansha) MIYA Akiko  Ebara Research Co Ltd., 第5研究員
OHMURA Tatsuo  Faculty of Engineering, Iwate University Associate Professor, 工学部, 助教授 (30111248)
KANEKO Hidehiro  Faculty of Engineering, The University of Tokyo Research Associate, 工学部, 助手 (60177524)
FUJITA Kenji  Faculty of Engineering, The University of Tokyo Professor, 工学部, 教授 (40107529)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1988: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsBacteriophage / Model Virus / Chlorination / Activated Sludge Particulate / Plaque Size / Composting Process / Inactivation of Virus / Coliphage QBETA / プラ-クサイズ / 大腸菌ファージ / 活性汚泥への吸着 / 生物膜への吸着 / ウィルスの不活化 / コンポスト化
Research Abstract

A new method was developed to evaluate the removal efficiency of viruses in water treatment processes using coliphages as a tracer. The results of this research are as follow. 1. Most of coliphages in sewage were found to be adsorbed onto suspended solids. The adsorbed coliphages could be easily eluted into the liquid phase with beef extract. The adsorption mechanism of viruses onto activated sludge particles was investigated in the laboratory-scale batch experiment using coliphage QBETA as model virus. The pH value and the microbial activity were found to be the influential factors. Coliphage QBETA was used also in the chlorination process and the ultraviolet irradiation process as model virus to evaluate the virus inactivation efficiency. 2. The biofilm process had less removal efficiency of coliphages than activated sludge process. Coliphages were adsorbed onto the biofilms and survived there without inactivation. Classification of coliphages was attempted on the plaque size (-1mm, 1-3mm, 3mm-) using E.coli B as a host organism in the activated sludge process. 3. The survival and inactivation of viruses in the composting process was investigated using coliphage QBETA as a model virus. The inactivation process of QBETA in the compost was expressed using first order kinetics considering temperature and water content.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Prasang Mongkonsiri: "Coliphage(Qβ)Inactivation by Free-Chlorine and Protective Effects of Activated-Sludge Particles" 第26回衛生工学研究討論会講演集(自由投稿発表部門). 92-94 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ketratanakul.A: "Adsorption of RNA-F-specific coliphages Qβ,on Suspended solids in activated sludge" Water Pollution Control in Asia,Pergamon. 321-327 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 大村達夫: "下水処理場における指標微生物の除去" 衛生工学研究論文集. vol.25. 129-137 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 金子栄廣: "下水汚泥中における大腸菌ファ-ジQβの熱による不活化" 土木学会第43回年次学術講演会講演概要集. 第43回. 962-963 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 大垣眞一郎: "生活環境系における病原微生物の制御に関する基礎的研究" 浄化層技術研究集会 講演要旨集. 第2回. 39-43 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] ケトラタナクル(ketratanakul,A): "Occurrence of Coliphages and RNA-F-Specific Coliphages in Sewages" 環境科学シンポジウム講演報告集. 52 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ketratanakul, A., Ohgaki, S.: "Indigenous Coliphages and RNA-F-Specific Coliphages Associated with Suspended Solids in the Activated Sludge Process." Wat. Sci. Tech.21, 3. 73-78 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ketratanakul, A., Ohgaki, S.: "Adsorption of RNA-F-Specific Coliphages, QBETA, on Suspended Solids in Activated Sludge." Water Pollution in Asia. 321-327 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohgaki, S., Nagaoka, H., Sekiya, T, and Izumino, M.: "Evaluation of Ultraviolet Light Disinfection Design Using Bacteriophages as Bioindicator." Selected Topics on Clean Technology, Proceedings of Workshop on Clean Technology, Asian Institute of Technology, Bangkok, Thailand, 6-8 Nov, 1989. 115-123

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ketratanakul, A., Ohgaki, S.: "Occurrence of Coliphages and RNA-F-Specific Coliphages in Sewage." Proc. Envr. Sci. Symp.52. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mongkonsiri, P., Ohgaki, S.: "Coliphage (QBETA) Inactivation by Free-Chlorine and Protective Effects of Activated Sludge Particulate." Proc. Environ. & Sanit. Eng. Res.26. 92-94 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohmura, T., Umita, T., Aizawa, J., Yagi, T., and Onuma, M.: "Removals of Indicator Microorganisms in Sewage Treatment Plants." Proc. Environ. & Sanit. Eng. Res.25. 129-137 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kaneko, H., Konoki, K., and Fujita, K.: "Inactivation of Coliphage QBETA in Sewage Sludge." Proc. JSCE. 962-963 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Prasang Mongkonsiri: "Coliphage(Qβ)Inactivation by Free-Chlorine and Protective Effects of Activated-Sludge Particles" 第26回衛生工学研究討論会講演集(自由投稿発表部門). 92-94 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ketratanakul.A: Water Pollution Control in Asia,Pergamon. 321-327 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 大村達夫: 衛生工学研究論文集. VOL.25. 129-137 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 金子栄廣: 土木学会第43回年次学術講演会講演概要集. 第43回. 962-963 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 大垣眞一郎: 浄化槽技術研究集会講演要旨集. 第2回. 39-43 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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