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Study on Environmental Control for Prevention against Sudden Population Decrease in Mass Production of the Rotifer Brachionus plicatilis.

Research Project

Project/Area Number 62860024
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionFACULTY OF FISHERIES, NAGASAKI UNIVERSITY

Principal Investigator

HIRAYAMA Kazutsugu  FACULTY OF FISHERIES, NAGASAKI UNIVERSITY. PROFESSOR., 水産学部, 教授 (80039718)

Co-Investigator(Kenkyū-buntansha) MAEDA Masachika  OCEAN INSTITUTE, UNIVERSITY OF TOKYO. ASSOCIATE., 海洋研究所, 助手 (40107454)
HINO Akinori  FACULTY OF AGRICULTURE, UNIVERSITY OF TOKYO. ASSOCIATE PROFESSOR., 農学部, 助教授 (90012012)
IMADA Katsumi  MARINE BIOTECHNOLOGY INSTITUTE Co. Ltd. VICE DIRECTOR., 副所長 (10112327)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥18,400,000 (Direct Cost: ¥18,400,000)
Fiscal Year 1989: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1988: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1987: ¥7,200,000 (Direct Cost: ¥7,200,000)
KeywordsDetritus food chain / Vitamin producing bacteria / Baker's yeast / Brachionus plicatilis / Freshwater Chlorella / Protozoa / Control of bacteria flora / Seedling / 餌料生物 / 腐食々物連鎖 / 種皮生産 / ビタミンB_<12> / 細菌
Research Abstract

The rotifer Brachionus plicatilis was found to have requirements for vitamin C and cholesterol when evaluated using basic food suspension composed of baker's yeast and other essential vitamins. Two strains of marine yeasts were found to have higher food value for the rotifer than baker's yeast. Freshwater Chlorella enriched with vitamin B_<12> showed the food value which could match that of marine Chlorella. Stable mass production of the rotifer was achieved by the supply of the freshwater Chlorella enriched with vitamin B_<12>.
Bacteria in a rotifer pond are proved to be important as an energy source and a supplier of essential vitamins. On the contrary, the venomous bacteria were found in mass culture pond of the rotifer which introduce sudden mass mortality. The rotifer culture was improved to attain extremely high density when bacterial flora was controlled by adding some useful bacteria periodically to the pond.
Most of ciliated protozoa occurred in the culture water of the rotifer were identified as Uronema and Euplotes. These ciliates nurtured the certain bacterial flora, which repressed the growth of the rotifer. These ciliates could be removed by installing the substratum in the culture water of the rotifer where the protozoa tended to attach. Using Artemia at the growth stage of more than the 10th instar, protizoa were also removed by its feeding. The growth of flagellated protozoa which seriously fed on marine Chlorella could be repressed by adding NaCl at the final concentration more than 3 % in water.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] K.HIRAYAMA: "Nutritional effect of freshwater Chlcrella on growth of the rotifer Brachionus plicatilis" Hydrobiologia. 186/187. 39-42 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 丸山功: "Uptake of vitamin B_<12> by various strains of unicellulas algae Chlorella" 日本水産学会誌. 55(10). 1785-1790 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] C.G.Satuito: "Nutritional quality of two speies of marine yeast on the population growth of the rotifer" Proceeding of the 2nd Asian Fisheries Forum. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 丸山功: "ビタミンB_<12>含有クロレラ給餌によるシオミズツボワムシの開放培養" 水産増殖. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 兼松正衛: "Nannochloropsisを摂食する鞭毛虫の駆除法について" 日本水産学会誌. 55(8). 1349-1352 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 前田昌調: "海洋および種苗生産過程に出現する原生動物・繊毛虫類" 栽培技研. 16(2). 155-178 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] J.P.YU: "Vitamin B_<12>-Producing Bacteria as a Nutritive Complement for a Culture of the Rotifer Brachionus Plicatilis" 日本水産学会誌. 54. 1873-1880 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] J.P.YU: "Function of Bacteria as Vrtamin B_<12> Producers during Mass Culture of the Rotifer Brachionus plicatilis" 日本水産学会誌. 55. 1799-1806 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 宇城正和: "シオミズツボワムシの微生物捕食とその意義" 月刊「海洋」. 22. 20-27 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] J.P.YU: "Toxicity of Vibrio alginolyticus on the Survival of the Rotifer Brachionus plicatilis" 日本水産学会誌. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] J.P.YU: "The Role of Bacteria in Mass Culture of the Rotifer Brachionus plicatilis" Proceedings of the 2nd. Asian Fish. Forum. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.USHIRO: "Energy Feedback in the Culture of the Rotifer Brachionus plicatilis" Proceedings of the 2nd. Asian Fish. Forum. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 平山和次: "初期餌料生物ーシオミズツボワムシ(福所邦彦・平山和次編)" 恒星社厚生閣, 240 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazutsugu HIRAYAMA et al: "Nutritional Effect of Freshwater Chlorella on Growth of the Rotifer Brachionus plicatilis." Hydrobiologia. 186/187.39-42.1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Isao MARUYAMA et al: "Uptake of Vitamin B_<12> by Various Strains of Unicellular Algae Chlorella." Nippon Suisan Gakkaishi. 55.1785-1790.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Cyril Grenn Satuito and K. HIRAYAMA: "Nutritional Quality of Two Species of Marine Yeast on the Population Growth of the Rotifer Brachionus plicatilis." Proceeding of the 2nd Asian Fisheries Forum. In press. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Iao MARUYAMA et al: "Effect of Chlorella Enriched with Vitamin B_<12> as a Food for Mass Production of the Rotifer Brachionus plicatilis." Suisannzoshoku. Submitted.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Jian-Ping YU et al: "Vitamin B_<12>-producing Bacteria as a Nutritive Complement for a Culture of the Rotifer Brachionus plicatilis." Nippon Suisan Gakkaishi. 54.1873-1880.1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Jian-Ping YU et al: "Function of Bacteria as Vitamin B_<12> Producers during Mass Culture of the Rotifer Brachionus plicatilis." Nippon Suisan Gakkaishi. 55.1799-1806.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Jian-Ping YU et al: "Toxicity of Vibrio alginolyticus on the Survival of the Rotifer Brachionus plicatilis." Nippon Suisan Gakkaishi. In press. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Jian-Ping YU et al: "The role of Bacteria in Mass Culture of the Rotifer Brachionus plicatilis." Proceeding of the 2nd Asian Fishers Forum. In press. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Masakazu USHIRO et al: "Energy Feedback in the Culture of the rotifer Brachionus plicatilis." Proceeding of the 2nd Asian Fishers Forum. In press. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Masaei KANEMATSU et al: "Methods to Repress the Growth of a Nannochloropsis-grazing Microflagellate." Nippon Suisan Gakkaishi. 55.1349-1352.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Masachika MAEDA: "Cilliata, Protozoa Appearance in the Ocean and in a Pond for Seedlings." Saibaigigen. 16.155-178.1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.HIRAYAMA: "Niitritional effet of fresh water Chlorella on growty of the rofifer Brochionus plicatilis" Hydrobiologia. 186/187. 39-42 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 丸山功: "Uptake of vitamin B_<12> by varicus strains of unicellular atgae chlorella" 日本水産会学誌. 55C10. 1785-1790 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] C.G.Satuito: "Nutritional quality of two speties of matine yeast on the population grouth of the rotifer" Proceeding of the 2td Asian Fisheries Forum. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 丸山功: "ビタミンB_<12>に含有クロレラ給餌によるシオミズツボワムシの開放培養" 水産増殖. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] J.P.YU: "Function of Bacteria as Vitamin B_<12> Producers during Mass Culture of the Rotifer Brachionus plicatilis" 日本水産学会誌. 55. 1799-1806 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 宇城正和: "シオミズツボワムシの微生物捕食とその意義" 月刊「海洋」. 22. 20-27 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] J.P.YU: "Toxicity of Vibrio alginolytlcus on the Survival of the Rotifer Brachionus plicatilis" 日本水産学会誌(投稿中). (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] J.P.YU: "The Role of Bacteria in Mass Culture of the Rotifer Brachionus plicatilis" Proceedings of the 2nd.Asian Fish Forum. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.USHIRO: "Enegry Feedback in the Culture of the Rotifer Brachionus plicatilis" Proceedings of the 2nd.Asian Fish Forum. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 兼松正衛: "Nannochlorisを摂食する鞭毛虫の駆除法について" 日本水産学会誌. 55(8). 1349-1352 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 平山和次: "初期餌料物ーシオミズツボワムシ(福所邦彦、平山和次編" 恒星社厚生園, 240 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 干建平: 日本水産学会誌. 54. 1873-1880 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 平山和次: 昭和63年度栽培漁業技術研修事業基礎理論コース 餌料生物シリーズ. No.8. 1-16 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 丸山功: 日本水産学会誌.

    • Related Report
      1988 Annual Research Report
  • [Publications] 前田昌調: 日本水産学会誌.

    • Related Report
      1988 Annual Research Report
  • [Publications] 干建平: 第2回アジア水産学会報告.

    • Related Report
      1988 Annual Research Report
  • [Publications] Satuito C.G.: 第2回アジア水産学会報告.

    • Related Report
      1988 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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