| Project/Area Number |
62860033
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
農業気象・生物環境制御学
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| Research Institution | Faculty of Horticulture, Chiba University |
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Principal Investigator |
KOZAI Toyoki Faculty of Horticulture, Chiba University, 園芸学部, 助教授 (90081570)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Makio Graduate School of Science and Technology, Chiba University, 大学院・自然科学研究科, 助手 (60092087)
NITO Nobumasa Faculty of Agriculture, Saga University, 農学部, 助教授 (80015809)
MII Masahiro Faculty of Horticulture, Chiba University, 園芸学部, 助教授 (30093074)
OGURA Yuko Faculty of Agriculture, Utsunomiya University, 農学部, 教授 (90114157)
SUGI Jiro Nodai Research Institute, Tokyo University of Agriculture, 総合研究所, 名誉教授 (20078231)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥21,200,000 (Direct Cost: ¥21,200,000)
Fiscal Year 1988: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1987: ¥15,000,000 (Direct Cost: ¥15,000,000)
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| Keywords | Plant tissue culture / Sugar free culture / Photoautotrophic culture / Carnation / Carbon dioxide enrichment / Micropropagation / Strawberry |
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| Research Abstract |
We developed a prototype of photoautotrophic plant micropropagation system. With this system, environmental factors such as temperature, humibity, light intensity and PH of nutrient solution can be controlled. We conducted a series of test run and proved that the environment can be controlled with desired ranges.
A series of experiments was conducted under different envirironmental conditions in order to find favorable environmental conditions for promoting the growth of plantlets in vitro at multiplication and rooting stages. Carnations, strawberries, potato etc. were used as plant materials.
Then, we found that explants with a small leaf containing chlorophyll can be differentiated and grown photoautotrophically with sugar-free medium and that the growth is much promoted in photoautotrophic culture than in heterotrophic culture.
Photoautotrophic micropropatation may have essential advantages against conventional heterotrophic micropropagation. Namely, a loss of plantlets due to contgamination can be reduced; a larger vessel in size can be used, automation of the micropropagation process can be realized, ctc.
Energy analysis, growth analysis and matter production balance analysis were conducted in order to obtain the basic knowledge on the development of automated and energy- and labor saving photoautotrophic masspropagation system for tissue-cultured plantlets.
Photosynthetic characteristics of tissue-cultured plantlets were measured for strawberry, potato and carnation. We found that their characteristics were basically similar to those for the plants grown exvitro.
Based on the experimental results obtained through the present research we conclude that the photoautotrophic masspropagation is feasible on a commercial scale in the near future.
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