| Project/Area Number |
62860038
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KANAGAWA Hiroshi Faculty of Veterinary Medicine, Hokkaido University, Professor, 獣医学部, 教授 (00111162)
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| Co-Investigator(Kenkyū-buntansha) |
UDAKA Kenji Embryo Transfer Research Laboratory, Snow Brand Milk Products Co. Ltd., Research, 受精卵移植研究所, 研究員
TOGASHI Hisashi Embryo Transfer Research Laboratory, Snow Brand Milk Products Co. Ltd., Research, 受精卵移植研究所, 研究員
HISHINUMA Mitsugu Faculty of Veterinary Medicine, Hokkaido University, Instructor, 獣医学部, 助手 (30183578)
TAKAHASHI Yoshiyuki Faculty of Veterinary Medicine, Hokkaido University, Assistant Professor, 獣医学部, 助教授 (70167485)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1989: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Cow / Embryo / Bisection / Identical twin / Mouse |
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| Research Abstract |
1. Bisection and sex determination by chromosome analysis on mouse embryos Eight-cell stage embryos, decompacted morulae and early blastocysts were bisected by using a fine, glass microneedle. The rates of development up to the blastocyst stage were 51.4, 78.4 and 27.8 %, respectively. After decompacted morulae were bisected, the condition of making chromosome analysis preparation were discussed. It was found that the optimum duration of in vitro culture was 15-18 hours and treatment with colcemid was 3 hours.
2. Bisection and sex determination by chromosome analysis on bovine embryos Sixty-five morulae and blastocyst stage embryos were bisected and chromosome analysis was conducted on half of the demi-embryos. Sex was determined in 38.5 % of the bisected embryos. Calves from sexed-bisected embryos grew normally and milk production was normal.
3. Production of bovine twins Two non-bisected embryos were either transferred into a single uterine horn or one embryo on each horn. There was no significant difference on the pregnancy rates which were 62.4 and 69.4 %, respectively. The abortion rate for single horn transfer was 20.6 %.
4. Production of bovine identical twins The bisected embryos were transferred either by twin or single transfer. The pregnancy rates were 72.6 and 61.5 %, respectively. In twin and single transfers, 71.0 and 23.1 % resulted in pregnancy, respectively. Identical twins were produced in 45.2 and 15.4 % in twin and single transfers, respectively. Many stillbirth and abortion cases were observed when single transfer was conducted.
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