| Project/Area Number |
62870018
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Nihon University |
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Principal Investigator |
SHIKATA Toshio Nihon University, Medicine, Professor, 医学部, 教授 (50009932)
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| Co-Investigator(Kenkyū-buntansha) |
MAENO Mitsugu Nihon University, Medicine, Assistant, 医学部, 助手 (10190315)
SUZUKI Koyu Nihon University, Medicine, Lecturer, 医学部, 講師 (00158974)
ESUMI Mariko Nihon University, Medicine, Associate Professor, 医学部, 助教授 (60160363)
UCHIDA Toshikazu Nihon University, Medicine, Associate Professor, 医学部, 助教授 (80060078)
SHIMIZU Yohko National Institute of Health Hepatitis Research Unit, Researcher, 腸内ウイルス部, 主任研究官 (00142357)
阿部 賢治 国立予防衛生研究所, 研究官 (60130415)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1987: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | Non-A, non-B hepatitis / Hepatitis C / Monoclonal antibody / 48-1 antigen and antibody / PCR method / PCR / C型肝炎関連抗体 / 輸血後の非A非B型肝炎 / 抗原抗体系 / microtubular aggregate |
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| Research Abstract |
An attempt was made to establish an antigen antibody assay system associated with non-A, non-B hepatitis. We have established monoclonal antibody from convalescent chimpanzee lymphocytes by EV virus transformation method. We found the corresponding antigen in the chimpanzee liver infected with non-A, non-B hepatitis virus and never found in the liver of the chimpanzees infected hepatitis A and B virus.
However, cDNA coding this antigen hybridized with somatic genome, then this antigen was not coded by the viral genome.
Then we cloned hepatitis C Genome from Japanese donor blood by immunoscreening and sequenced. Sequence of this Japanese clone of HCV is different from Chiron's clone. Hemology is only 74% in region NS5. We established EIA assay system of the antibody against HCV.
And we also established cDNA assay system of HCV genom by PCR method.
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