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Development and application of ELISA using a monoclonal antibody against -glutamyltransferase sugur chains

Research Project

Project/Area Number 62870030
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionOsaka University Medical School

Principal Investigator

TANIGUCHI Naoyuki  Professor Osaka University Medical School, 医学部, 教授 (90002188)

Co-Investigator(Kenkyū-buntansha) KAMADA Takenobu  Professor Osaka University Medical School, 医学部, 教授 (80028399)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥7,000,000 (Direct Cost: ¥7,000,000)
Keywords-glutamyltransferase / 糖鎖 / モノクローン抗体 / 肝癌 / γーグルタミルトランスペプチダーゼ
Research Abstract

A monoclonal antibody, gammas-54-29 was raised against sugar chains of highly purified gamma-glutamyltransferase (gammaGT) from human primary hepatoma. The antibody preferentially bound to the small subunit of gammaGT from human hepatoma and kidney as evidenced by immunoblot analysis. Weak binding to the normal liver enzyme could be detected by solid-phase enzyme-linked immunosorbent assay (ELISA). With the use of this antibody, an ELISA was developed for the quantitation of immunoreactive gammaGT in human serum. Sera of 188 normal control subjects displayed a low level (9.4 mug/ml) of immunoreactive gammaGT. Highly elevated levels of immunoreactive gammaGT were detected in the sera of patients with primary hepatoma, metastatic liver cancer and hepatitis. The levels of gammaGT were within a normal range in the sera of patients with gastrointestinal cancers and patients with nonmalignant diseases such as liver cirrhosis and gallstones. The antibody has been shown to be useful for the diagnosis of some of the neoplastic diseases.

Report

(2 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Arai,K.: J.Biol.Chem.262. 16969-16972 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nishikawa,A.: Anal.Biochem.170. 349-354 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nishikawa,A.: Biochem.Biophys.Res.Commun.152. 107-112 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sakamuro,D.: Gene. 73. 1-9 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ishibashi,K.: Clin.Chim.Acta. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Taniguchi,N.: Methods Enzymol.(1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Arai. K.: "Glycation and inactivation of human Cu-Zn-superoxide dismutase: Identification of the in vitro glycated sites." J. Biol. Chem.262. 16969-16972 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nishikawa, A.: "A method for the determination of N-acetylglucosaminyltransferase III activity in rat tissues involving HPLC." Anal. Biochem.170. 349-354 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nishikawa, A.: "High expression of an N-acetylglucosaminyltransferase III in 3'-methyl DAB-induced hepatoma and ascites hepatoma." Biochem. Biophys. Res. Commun.152. 107-112 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sakamuro, D.: "The primary structure of human gamma-glutamyl transpeptidase." Gene. 73. 1-9 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ishibashi, K.: "N-acetylglucosaminyltransferase III in human serum and liver and hepatoma tissues: Increased activity in patient with liver cirrhosis and hepatomas." Clin. Chim. Acta. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Arai,K.: J.Biol.Chem.262. 16969-16972 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nishikawa,A.: Anal.Biochem.170. 349-354 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nishikawa,A.: Biochem.Biophys.Res.Commun.152. 107-112 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sakamuro,D.: Gene. 73. 1-9 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ishibashi,K.: Clin.Chim.Acta. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Taniguchi,N.: Methods Enzymol.(1989)

    • Related Report
      1988 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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