| Project/Area Number |
62870049
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
MITO Micho Asahikawa Medical College, Professor, 医学部, 教授 (60000981)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Tetsu Asahikawa Medical College, Assistant, 医学部, 助手 (50125415)
ASAKAWA Hiroichi Asahikawa Medical College, Assistant, 医学部, 助手 (80125393)
KASAI Shinichi Asahikawa Medical College, Assistant-Professor, 医学部, 講師 (40091566)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 1988: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1987: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Isolated hepatocytes / Entrapped hepatocytes / Collagenase digestive method / Calcium alginate gel / Hepatic failure / 人工肝臓 |
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| Research Abstract |
Thre is a limit to what can be achieved in treating patients with fulminant heptic failure as functional failure of the liver due to rapid, extensive hepatic cell necrosis. Resently, various artificial liver supports have been applied clinically to treat the disease. Although the patient's state of consciousness has improved remarkably, the survival rate is still low. We lack a good understanding of the pathophisiology of the disease and an effective therapeutic means of supporting the metabolic function of the damaged liver. Therefore, the hybrid artificial liver has been investigated for metabolic support system. The main problems with the system are maintenance of function, survival time of the hepatocytes, and many systems are studied for the purpose. Entrapping the hepatocytes within calcium alginate is one of the expected method. The purpose of this project is to develop the device to antrap the hepatocytes automatically within the gel.
1. Cell isolation. Parenchymal hepatocytes were isolated from dog, pig and calf by collagenase digestive method.
2. Development of the device. Amixture of cell suspension and 1 to 2% sodium alginate was automatically dropped into 0.1m CaCl_2 through a 1.5mm nozzle of the newlly developpmental device. About 200ml of hepatocytes are entrapped for 30 minutes.
3. Function of entrapped hepatocytes. Metabolic function and it's maintenance were examined by in-vitro perfusion experiments, and good results were obtained. Ammonia detoxification function was better than that of cultured hepatocytes. Other chemical reactions were maintained almost same levels as culture system. Mechanical strength of the beads was enough tolerable in condition with the rotator for five days.
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