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Developement of abiwave stopped flow fluorophotometer

Research Project

Project/Area Number 62870074
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TANIGUCHI Kazuya  Dept. Pharmacol. Sch. Dentistry, Hokkaido University, 歯学部, 助教授 (40028204)

Co-Investigator(Kenkyū-buntansha) NAGAMURA Toshihiko  UNISOKD, 取締役
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsNa^+ pump / Na^+ / K^+-ATPase / Na^+;K^+-ATPase
Research Abstract

Following results were obtained 1,The instrument permits to follow two different fluorescence changes with time scale of 50 msec to up to 30 sec with 0.12ml of the sample volume. 2,ATP did no bind to E1P in such a way as to accelerate the breakdown of the phosphoenzyme 3,The site of attachment of BIPM probe was shown to be Cys 964 of the alpha chain of Na^+ pump. 4,Changes in fluorescence energy transfer between sulfhydyl fluorescence probes occured out of phase, which showed dynamic confotmational changes during active transport of Na^+ and K^+. 5,Binding of Mg^<2+>, Na^+ and ATP to Na^+ pump induced the conformational change. Na^+ occlusion by oligomycin also induced the similar change. These data strongly suggest that the role of Mg ATP is to occlude Na^+.

Report

(2 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Kuniaki Suzuki:Kazuya Taniguchi:shoichi Iida: J.Biol.Chem.262. 11752-11757 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masahiko Sakuraya:Kazuya Taniguchi:Kuniaki Suzuki et.al: Jap.J.Pharmacol.44. 311-321 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kazuya Taniguchi.:Hiroyuki Tosa.:Kuniaki Suzuki.:Yoshiko Kamo: J.Biol.Chem.263. 12943-12947 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 谷口和弥: 蛋白質・核酸・酵素. 33. 2496-2507 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 谷口和弥: 代謝. 26. 3-11 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kazuya Taniguchi.;Kuniaki Suzuki;Momohiko Nagai,et.al.: "Perspectives of Biological Energy Transduction eds.Mukohata,Morales and Fleisher" Academic Press, 6 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kazuya Taniguchi.;Kuniaki Suzuki;Toshio Sakaki;Hiroyuki Tosa;Eiji Shinoguchi: "The Na^+、K^+-Pump." Alan R.Liss,Inc, 8 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kazuya,Taniguchi; Kuniaki,Suzuki; Toshio,Sasaki; et al.: "Acceleration of the rate of fluorescence decrease by high concentrations of ATP under the condition of accumulation of ADP-sensitive phosphoenzymes Na^+ , K^+ -ATPase" J.Biochem. 100. 1231-1239 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Momohiko,Nagai; Kazuya,Taniguchi; Kenji,Kangawa; Hisayuki,Matsuo; et al.: "I dentificaiton of N-[p-2-benzimidazolyl) phenyl] maleimide-modified residue participating in Dynamic fluorescence changes Accompanying Na^+ , K^+ - dependent ATP hydrolysis" J.Biol. Chem.261. 13197-13202 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kuniaki,Suzuki; Kazuya,Taniguchi; Shoichi,Iida: "The acceleration of Na^+ , K^+ -ATPase activity by ATP and ATP analognes." J.Biol. Chem.262. 11752-11757 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masahiko,Sakuraya; Kazuya,Taniguchi; Kuniaki,Suzuki; et. al: "Changes in Fluorescence Energy Transfer between Sulfhy dryl Fluorescence Residues during ouabain sensitive Na^+ , K^+ -ATP hydrolysis" Jap. J. Pharmacol.44. 311-321 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kazuya,Taniguchi; Hiroyuki,Tosa; Kuniaki,Suzuki; Yoshiko,Kamo: "Microenvironment of two different extrinsic fluorescence Probes in Na^+ , K^+ -ATPase changes Out of Phase during Sequential Appearance of Reaction Intermediates" J. Biol. Chem.263. 12943-12947 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kazuya,Taniguchi; Kuniaki,Suzuki; Momohiko,Nagai; et. al.: Perspectives of Biological Energy Transduction eds. Mukohata, Morales and Fleisher. Academic Press, 6 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kuniaki Suzuki;Kazuya Taniguchi;Shoichi Iida: J.Biol.Chem.262. 11752-11757 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Masahiko Sakuraya;Kazuya Taniguchi;Kuniaki Suzuki;et al.: Jap.J.Pharmacol.44. 311-321 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kazuya Taniguchi;Hiroyuki Tosa;Kuniaki Suzuki;Yoshiko Kamo: J.Biol.Chem.263. 12943-12947 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 谷口和弥: 蛋白質・核酸・酵素. 33. 2496-2507 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 谷口和弥: 代謝. 26. 3-11 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kazuya Taniguchi;Kuniaki Suzuki;Momohiko Nagai;et al.: "Perspectives of Biological Energy Transduction eds.Mukohata,Morales and Fleisher" Academic Press, 6 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kazuya Taniguchi;Kuniaki Suzuki;Toshio Sasaki;Hiroyuki Tosa;Eiji Shinoguchi: "The Na^+,K^+-Pump." Alan R.Liss,Inc., 8 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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