| Project/Area Number |
62870095
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Kumamoto University |
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Principal Investigator |
KUBOTA Yukiho Blockage of morphological transforation of chick embryo fibroblasts infected with Rous sarcoma virus by N__--myristoyl glycinal diethylacetal, 薬学部, 教授 (30040299)
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| Co-Investigator(Kenkyū-buntansha) |
KAGIMOTO Tadashi Physical treatment of human T-cell leukemia virus-infected cells with N__--myris, 医療技術短期大学部, 教授 (30040273)
YOSHIKAWA Hiromi Effect of N__--myristoylpeptide derivatives on human hepatitus B virus, 主任研究員
KUWATA Shigeki Effect of N__--myristoyl peptide derivatives on human T-cell leukemia virus, 主任研究員
SHOJI Shozo Synthesis, analysis, and biological activity of NH_2-terminal myristoyl peptide, 薬学部, 助教授 (60040317)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1988: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1987: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Myristoyl peptide / retrovirus / human retroviruses / HTLV-I / HIV / レトロウイルス |
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| Research Abstract |
N-myristoyl glycinal diethyacetal, CH_3(CH_2)_<11>CH_2-CONHCH_2(CO_2H_5)_2, which is abbreviated as N-MYr-GOA, and the other N-Myr-compounds were newly synthesized and then employed for a NH_2-terminal antimyristoylation of structure proteins in the human T-cell leukemia virus(HTLV-I) and the human immuno-deficiency virus(HIV). The compounds were also tested for reverse transformation of chick embryo fibroblast(CEF) infected with a temperature-sensitive mutant(tsNY68) of Rous sarcoma virus(RSV).
of the compounds tested, N-Myr-GOA extremely inhibited protein myristoylation of p19^<gag> of HTLV-I, p17^<gag> of HIV-I^1, and pp60^<v-src>, which is a transforming protein of tsNY68. N-Myr-GOA also prevented morphological transformation of tsNY68-infected-CEF, and nonmyristoylated pp60^<v-src> caused reverse transformation of the infected-CEF.
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