| Project/Area Number |
63041013
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| Research Category |
Grant-in-Aid for Overseas Scientific Survey.
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| Allocation Type | Single-year Grants |
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| Section | Field Research |
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| Research Institution | Faculty of Agriculture, Iwate University |
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Principal Investigator |
SAKAMOTO Tsukasa Professor, Faculty of Agriculture, Iwate University, 農学部, 教授 (80001527)
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| Co-Investigator(Kenkyū-buntansha) |
G.W Hutchins ゼームスクック大学, 北クィーンズランド校熱帯獣医学部, 上級講師
R.C.A Thomps マードック大学, 獣医学部, 準教授
ITAGAKI Tadashi Research Associate, Faculty of Agriculture, Iwate University, 農学部, 助手 (80203074)
KAWASE Shiro Chief, Laboratory of Experimental Animal, Department of Epidemiology, Hikkaido I, 疫学部実験動物室, 室長
THOMPSON R.C.A. Associate Professor, School of Veterinary Studies, Murdoch University
HUTCHINSON G.W. Senior Lecturer, Graduate School of Tropical Veterinary Science, James Cook Univ
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1989: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | Australia / Unilocular Echinococcosis / Intraspecific Variation / Beef cattle / Kangaroo / Feral Pig / DNA probe / Immunological Diagnosis |
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| Research Abstract |
Intraspecific variations in Echinococcus granulosus were analysed on the basis of electropholetic expressions of isozymes in the hydatid materials of sheep and cattle from New South Wales (NSW), Victoria (Vic), Tasmania (Tas), Western Australia (WA) and Queensland (Qld). Major differences were found at 9 enzyme loci between the isolates of the Australian mainland and Tasmanian sheep strains. Purified total genomic DNA labeled with photobiotin was confirmed to be useful as a probe for the identification and differentiation of strains of E. granulosus. Hydatid cysts were found from black striped wallabies of macropod marsupials in Qil. Hydatid cysts were found in 20% of 54 feral pigs from Qid. Also, hydatid cyst were seen in 29% of 21 western grcy kangaroos and 46% of 24 feral pigs from WA. Active formation of protoscoleces was seen in hydatid cysts from western and eastern grey kangaroos. The hydatid cysts from other macropod marsupials were small and steile. Active protoscolex-formation was recognized in a few of the infected pigs. Most of the hydatid cysts in feral pigs were small, sterile and regressive. Adult hydatid worms were found in 47% of 19 foxes.
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