| Project/Area Number |
63044059
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
MORITA Yukitomo 1. Department of Physiology, Hamamatsu University School of Medicine, 医学部, 教授 (80034164)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA Katsuhisa 1. Department of Physiology, Hamamatsu University School of Medicine, 医学部, 助手 (10168693)
TAMOTSU Satoshi 1. Department of Physiology, Hamamatsu University School of Medicine, 医学部, 助手 (60188448)
SAMEJIMA Michikazu 1. Department of Physiology, Hamamatsu University School of Medicine, 医学部, 助手 (80135251)
SINGH R バナラス大学(インド), 理学部
TAKAHASHI J. S. Department of Biological Sciences, Northwestern University, U. S. A., 医学部, 助教授
MEYERーROCHOW ワイカト大学, 理学部(ニュージーランド), 助教授
EBADI M. ネブラスカ大学, 医学部(米国), 教授
KORF H.W ギーセン大学, 医学部(ドイツ連邦共和国), 助教授
UECK M. ギーセン大学, 医学部(ドイツ連邦共和国), 教授
OKSCHE A. ギーセン大学, 医学部(ドイツ連邦共和国), 教授
DODT E. マックス, プランク研究所(ドイツ連邦共和国), 教授
SINGH Ramesh Department of Biology, Banaras Hindu University, India
MEYER-ROCHOW V. B. Department of Biological Sciences, University of Waikato, New Zealand
EBADI Manuchair Department of Pharmacology, University of Nebraska, U. S. A.
UECK Manfred Department of Anatomy, University of Giessen, F. R. G.
KORF Horst-W. Department of Anatomy, University of Giessen, F. R. G.
DODT Eberhart Max Planck Institute, Bad Nauheim, F. R. G.
OKSCHE Andreas Department of Anatomy, University of Giessen. F. R. G.
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 1990: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1989: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1988: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | Pineal Organ / Antarctic Fish / UV-receptor / Deep-brain Photoreceptor / Electrophysiology / Immunocytochemistry / Lamprey / Electric Fish |
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| Research Abstract |
Environmental photic information regulates the function of pineal organs. In order to elucidate basic mechanisms of pineal functions, the international and interdisciplinary cooperative research was undertaken between our laboratory and the scientists from Germany, U.S.A., New Zealand and India. Main findings are as follows.
1. Electrophysiological and immunocytochemical study of pineal organs and retina of the Antarctic fishes, Pagothenia and Trematomus, Showed that they are well-developed photosensitive organs.
2. UV-receptor was found electrophysiologically in the lamprey's pineal, showing the peak sensitivity at 380 nm.
3. Application of NADPH-diaphorase staining demonstrated 4 types of pineal neurons, which could be subdivided into 8 groups. Double immunostaining with antisera against seretonin and rod-opsin was successful. A new cell-type with rob-opsin positivity without processes was found. Cone-opsin immunoreactivity suggested the presence of deep-brain photoreceptors in the paraventricular organ of lamprey.
4. Amazonian electric fish had pineal organs. In the retina of electric eel, rod-opsin immunoreactivity was shown in outer segments of visual cells, but no serotonin-positive amacrine cells were demonstrated.
5. Pineal photoreceptors of the lamprey were studied electrophysiologically whether they are sensitive to magnetic field.
Intracellular recording revealed no change in the resting membrane potential and in photic responses.
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