| Project/Area Number |
63044065
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| Research Category |
Grant-in-Aid for Overseas Scientific Survey.
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Nagoya University |
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Principal Investigator |
MATSUO Seiichi The Third Department of Medicine, School of Medicine, 医学部, 助手 (70190410)
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| Co-Investigator(Kenkyū-buntansha) |
YUZAWA Yukio State University of New York at Buffalo, Pathology, バッファロー校, 客員教授
JAN Brentjen ニューヨーク州立大学, バッファロー校, 教授
GIUSEPPE And ニューヨーク州立大学, バッファロー校, 教授
ITO Yasuhiko The Third Department of Medicine, School of Medicine, 医学部, 医員
BRENTJENS Jan State University of New York at Buffalo, Pathology
ANDRES Giuseppe State University of New York at Buffalo, Pathology
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1989: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Glomerulonephritis / Immunopathology / Immune Complex / Lectin |
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| Research Abstract |
The aim of the project was to study the mechanisms of immune glomerular injury, particularly to study the events following the interaction of antibodies with antigen planted on the glomerular endothelium (GEN). For this purpose, a lectin was implanted on the GEN by isolated kidney perfusion technique and subsequent re-vascularization of perfused kidney. Acute glomerulonephritis was induced by the intravenous administration of anti-lectin antibodies. In case of HPA, granular HPA-anti-HPA immune deposits (IDs) were formed on the GEN immediately after antibody administration possively through the mechanisms of patching/shedding involving cytoskeleton. These IDs moved from the luminal aspect of the glomerular capillary wall to the subepithelial area suggesting that membranous-like lesion was inducible in this model. In addition, HPA-binding glycoprotein was purified from the plasma membrane of rat glomerular cells. Rabbit antibodies to this protein reacted specifically with the free surface of podocytes and the nature of the epitope(s) was thought to be pertinent to the understanding of some kind of human glomerulonephritis.
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