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Studies on the mechanism on the neuron specific gene expression.

Research Project

Project/Area Number 63044091
Research Category

Grant-in-Aid for Overseas Scientific Survey.

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionInstitute for Protein Research, Osaka University

Principal Investigator

MIKOSHIBA Katsuhiko  Institute for Protein Research, Osaka University, 蛋白質研究所, 教授 (30051840)

Co-Investigator(Kenkyū-buntansha) SUTCLIFFE J. Greggor  Research Institute of Scripps Clinic, 教授
OKANO Hideyuki  Institute for Protein Research, Osaka University, 蛋白質研究所, 助手 (60160694)
IKENAKA Kazuhiro  Institute for Protein Research, Osaka University, 蛋白質研究所, 助手 (00144527)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1989: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsRegulation of Gene Expression / Myelin Basic Protein / Glial Fibrally Acidic Protein / Neurofilament / Myelin Proteolipid Protein / In vitro Transcription
Research Abstract

Many kinds of cells are present in the vervous system and exhibit various characteristic functions. Most of these cells differentiate from a common ancestor, neuroepithelial cells, migrate to their defined positions, and form well-organized architecture of the nervous system. As a first step toward understanding molecular mechanisms involved in these processes, we have been identifying the elements which regulate the gene expression of the proteins specifically produced in the neural cells. The genes for myelin basic protein (MBP), an oligodendrocyte marker protein, and glial fibrally acidic protein, an astrocyte marker protein, were apparently activated by NF-1 like element(s), while the gene for neurofilament, a neuron-specific protein, seemed to be de-repressed in neuron. Myelin proteolipid protein (PLP) is produced spacially and timely similar to MBP, however, the regulatory mechanism involved in its gene expression seemed to be quite distinct from that of the MBP gene. Thus, the neural cells activate the genes in various ways, presumably because each gene expression has to finely respond to the environmental change in order to exhibit the complex functions of the nervous system.

Report

(1 results)
  • 1989 Final Research Report Summary
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 岡野栄之ら: "Gene organization and transcription of duplicated MBP genes of myelin deficient(shi^<mld>)mutant mouse." EMBO J.7. 77-83 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 池中一裕ら: "Myelin proteolipid protein gene structure and its regulation of expression in normal and jimpy mutant mice." J.Mol.Biol.199. 587-596 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 田村隆明ら: "Analysis of transcription control elements of the mouse myelin basic protein gene in HeLa cell extrancts:Demonstration of a strong NF1-binding motif in the upstream region." Nucl.Acid Res.16. 11441-11459 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 田村隆明ら: "Enhance of human polyoma JC virus contains nuclear factor 1-binding sequences;analysis using mouse brain nuclear extracts." Biochem.Biophys.Res.Comm.157. 419-425 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 岡野栄之ら: "Recombination within the upstream gene of duplicated myelin basic protein genes of myelin deficient(shi^<mld>)mouse results in the production of anti-sense RNA." EMBO J.7. 3407-3412 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 塩田千代ら: "Developmental profile and differential localization of mRNAs of myelin proteins(MBP and PLP)in oligodendrocytes in the brain and in culture." Developmental Brain Res.45. 83-94 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 田村隆明ら: "Tissue-specific in vitro transcription from the mouse myelin basic protein promoter." Mol.Cell.Biol.9. 3122-3126 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 田村隆明ら: "Preparation of large amounts of transcription competent nuclear extracts from mouse brain." Technique. 1. 33-36 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 御子柴克彦ら: "Molecular genetics of myelin proteolipid proteins and their expression in normal and jimpy mice.in “Myelination and Demyelination:Implication for Multiple Sclerosis."" Plenum Publ.Corporation, 12 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 御子柴克彦ら: "Structure and function of myelin protein genes.in “Annual Review of Neuroscience."" Annual Reviews Inc., (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Okano, Hideyuki: "Recombination within the upstream gene of duplicated myelin basic protein genes of myelin deficient (shi^<mld>) mouse results in the production of anti-sense RNA." EMBO J.7. 3407-3412 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shiota, Chiyo: "Developmental profile and differential localization of mRNAs of myelin proteins (MBP and PLP) in oligodendrocytes in the brain and in culture." Developmental Brain Res.45. 83-94 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miura, Masayuki: "Tissue-specific vitro transcription from the mouse myelin basic protein promoter." Mol. Cell. Biol.9. 3122-3126 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tamura, Taka-aki: "Preparation of large amounts of transcription competent nuclear extracts from mouse brain." Technique. 1. 33-36 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Okano, Hideyuki: "Gene organization and transcription of duplicated MBP genes of myelin deficient (shi^<mld>) mutantmouse." EMBO J.7. 77-83 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ikenaka, Kazuhiro: "Myelin proteolipid protein gene structure and its regulation of expression in normal and jimpy mutant mice." J. Mol. Biol.199. 587-596 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tamura, Takaaki: "Analysis of transcription control elements of the mouse myelin basic protein gene in HeLa cell extracts: demonstration of a strong NF1-binding motif in the upstream region." Nucl. Acid Res.16. 11441-11459 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tamura, Taka-aki: "Enhance of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts." Biochem. Biophys. Res. Comm.157. 419-425 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mikoshiba, Katsuhiko: Structure and function of myelin protein genes.Annual Reviews Inc., (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mikoshiba, Katsuhiko: Molecular genetics of myelin proteolipid proteins and their expression in normal and jimpy mice. in "Myelination and Demyelination: Implication for Multiple Sclerosis.". Plenum Publ. Corporation, 85-96 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary

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Published: 1988-04-01   Modified: 2016-04-21  

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