| Project/Area Number |
63044130
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| Research Category |
Grant-in-Aid for Overseas Scientific Survey.
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Kyorin University School of Medicine |
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Principal Investigator |
SHIOHARA Tetsuo Department of Dermatology, Kyorin University School of Medicine, 医学部皮膚科, 助教授 (10118953)
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| Co-Investigator(Kenkyū-buntansha) |
THOMAS Kuppe エール大学, 医学部皮膚科, 研究員
RICHARD Edel エール大学, 医学部皮膚科, 主任教授
EDELSON Richard Department of Dermatology, Yale University School of Medicine
KUPPER Thomas Department of Dermatology, Yale University School of Medicine
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Epidermotropic T-cell clones / Keratinocyte-derived cytokines / Cell adhesion molecules / GM-CSF / IL-6 / LFA-1 / ICAM-1 / Extracorporeal photochemotherapy / GvHD resistance / Thy 1^+ epidermal cell / Fixed drug eruption / ICAM-1 / 固定薬疹 |
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| Research Abstract |
1. Mechanism by which certain T cells preferentially migrate into the epidermis was investigated by using murine cloned T-cell lines with or without epidermotropic nature. Expression of GM-CSF receptor was observed on the surface of all epidermotropic T-cell clones tested, although the expression was not restricted to the epidermotropic T-cell clones. LFA-1 was preferentially expressed on the surface of epidermotropic T-cell clones, while non-epidermotropic T-cell clones were negative or very weakly positive for LFA-1 with one exception. Expression of GM-CSF receptor that enables T cells to proliferate in response to keratinocyte-derived GM-CSF and high levels of LFA-1 expression that allows T cells to adhere to epidermis may be a constitutive property of epidermotropic T cells.
2. Mice that had spontaneously recovered from the cutaneous GVHD induced by the intradermal injection of epidermotropic T-cell clones became resistant to subsequent attempts to induce the cutaneous GVHD by the T cells. The great increase in the number of Thy 1^+ epidermal cells following destruction of epidermal structures is important in protecting the epidermal integrity from an additional attack by T cells.
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