| Project/Area Number |
63044135
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Nihon University, School of Medicine |
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Principal Investigator |
SHIKATA Toshio Nihon University, School of Medicine, Professor, 医学部, 教授 (50009932)
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| Co-Investigator(Kenkyū-buntansha) |
XUEYI Cao Xinjiang Anti-epidemic Station, Director, 所長
SHRESTHA S. M. Beer Hospital, Hepatology Unit, Head, 部長
GUPTA H. All India Institute of Medical Science, 教授
TANDON B. N. All India Institute of Medical Science, Director, 教授
INABA Yutaka Juntendou University, School of Medicine, Professor, 医学部, 教授 (30010094)
SUZUKI Kouyu Nihon University, School of Medicine Associate Professor, 医学部, 助教授 (00158974)
ESUMI Mariko Nihon University, School of Medicine Associate Professor, 医学部, 助教授 (60160363)
ABE Kenji National Institute of Health, Chief Investigator, 主任研究官 (60130415)
UCHIDA Toshikazu Nihon University, School of Medicine, Associate Professor, 医学部, 助教授 (80060078)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥18,000,000 (Direct Cost: ¥18,000,000)
Fiscal Year 1990: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1989: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1988: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | Epidemic non-A, non-B hepatitis / Hepatitis E / Experimental infection / Hepatitis E virus gernome / Antibody assay system / Viremia |
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| Research Abstract |
An attempt was made to characterize and molecularly clone the putative virus of epidemic non-A, non-B hepatitis. First, experimental animal model was established using cynomolgus macaca. Cynomolgus is a good animal model, because if we inoculate enough amount virus intravenously, almost all monkeys developed acute hepatitis. Serial transmission experiments were also done successfully. We have found large amount of virus excrete into bile from 10 days after inoculation until peak of transaminase. In early stage, virus scattered in the bile but next stage virus particles spontaneously aggregated probably due to secretory lgA. We already cloned viral RNA from lambda gtll cDNA library using immunoscreening method. We also confirmed viremia in incubation stage by PCR method. Now we are preparing antibody assay kit using recombinant peptide. We are attempting to develop recombinant vaccine too.
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