| Project/Area Number |
63044148
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
MURATA Norio National Institute for Basic Biology, 基礎生物学研究所, 教授 (90011569)
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| Co-Investigator(Kenkyū-buntansha) |
ZIMMERMANN J. -L. CNE/Saclay, France, 研究員
MATHIS P. CNE/Saclay, France, 光合成研究部門, 部長
WEISBEEK P. J. Department of Molecular Cell Biology, State University of Ultrecht, The Netherla, 分子生物部門, 部長
星名 哲 金沢大学, 理学部, 助手 (50019486)
MIMURA Tetsuro Faculty of Science, University of Tokyo, 理学部, 助手 (20174120)
FUJITA Yoshihiko National Institute for Basic Biology, 基礎生物学研究所, 教授 (40013560)
FUKUZAWA Hideya Institute of Applied Microbiology, University of Tokyo, 応用微生物研究所, 助手 (30183924)
TAKABE Akihiro Faculty of Science and Engineering, Meijo University, 理工学部, 助教授 (80097766)
TAKAHASHI Yuichiro Faculty of Science, Okayama University, 理学部, 助手 (50183447)
SATOH Kimiyuki Faculty of Science, Okayama University, 理学部, 教授 (10032822)
KAWAMORI Asako Faculty of Science, Kwansei Gakuin University, 理学部, 教授 (40079661)
KATOH Sakae Faculty of Science, University of Tokyo, 理学部, 教授 (50011515)
UEHARA Kaku Faculty of Technoloqy, Osaka Prefectural University, 工学部, 講師 (60081329)
IZUI Katsura Faculty of Science, Kyoto University, 理学部, 助教授 (20025414)
ASADA Koji The Research Institute for Food Science, Kyoto University, 食糧科学研究所, 教授 (50027182)
HOSHINO Satouru Faculty of Science, Kanazawa University
POTRYKUS I. Institut fur Pflanzenwissenschaften, Suisse
OLSON J. Instiute of Biochemistry, Odense University, Denmark
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 1990: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1989: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1988: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | Photosynthesis / Photochemical reaction center complex / Photosystem I complex / Photosystem II complex / Photosynthesis gene / Electron transport / Photosynthetic bacterium / Phosphonolpyruvate carboxylase |
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| Research Abstract |
One of the most important processes of photosynthesis is the photochemical reaction that converts light energy into chemical energy. Various kinds of protein complexes, which perform the photochemical reactions, are available, and are used in this study. The results are summarized as follows : (1) Photosystem II complex - (a) Plastoquinone of the cyanobacterial photosystem II complex was substituted by many kinds of quinone derivatives, and we found that tetrahalobenzene most efficienty bound to the photosystem II complex and was a most efficient electron acceptor. (b) The oxygen-evolving activity of the photosystem II complex was greatly stabilized by glycinebetaine.
(2) Photosystem I complex - (a) Selective destruction of FeS centers of the photosystem I complex demonstrated that both FeS centers A and B are essential to the electron transport. (b) In the reconstitution experiment with liposomes, it was suggested that monogalactolipid accelerates the transfer of excitation energy from the light-harvesting chlorophyll protein to the photosystem I complex.
(3) Bacterial reaction center complex - (a) The reaction center complex from Heliobacterium contained two molecules of bacteriochlorophyll g' and two molecules of a chlorophyll a-like pigment. (b) We studied the absorption and the CD spectra of monomer, dimer, trimer and polymer of Bacteriochlorophyll c which were formed in water - saturated CCl_4.
(4) Photosynthesis genes - (a) We purified from germinating wheat an enzyme which methanolizes plastid DNA. (b) We cloned genes of the chloroplast genome which encode small polypeptides of the photosystem II complex. (c) The tissuespecific expression of phosphoenolpyruvate carboxylase (PEPcase) was studied by ligating the promoter region of PEPcase gene with a reporter gene. The transcription of the gene for the C_4 type, but not C_3 type, of PEPcase was enhanced by NH_3.
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