| Project/Area Number |
63045025
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | University-to-University Cooperative Research |
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| Research Institution | Miyazaki University |
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Principal Investigator |
ADACHI Taiji Prof. Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University., 農学部, 教授 (50040861)
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| Co-Investigator(Kenkyū-buntansha) |
ミラエリナ ネシコビッケ リュブリアナ大学, 理学部(ユーゴスラビア), 教授
オスロニク ボリス リュブリアナ大学, 生物工学部(ユーゴスラビア), 助手
BOHANEC Borut Lecturer Plant Genetics and Breeding Department, Biotechnical Faculty University, 生物工学部(ユーゴスラビア), 講師
ヤウ゛オルニク ブランカ リュブリアナ大学, 生物工学部(ユーゴスラビア), 教授
クレフト イウ゛ァン リュブリアナ大学, 生物工学部(ユーゴスラビア), 教授
IKEDA Hajime Rector, Miyazaki University, 農学部, 学長 (20038194)
TAKAKI Hiroshi Prof. Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyaz, 農学部, 教授 (90094078)
SUIKO Masahito Assoc. Prof. Applied Genetics and Biotechnology Division, Faculty of Agriculture, 農学部, 助教授 (00128357)
KISHIMA Yuji Assist. Prof. Applied Genetics and Biotechnology Division, Faculty of Agricultur, 農学部, 助手 (60192556)
YABUYA Tsutomu Assoc. Prof. Applied Genetics and Biotechnology Division, Faculty of Agriculture, 農学部, 助教授 (70112414)
NESKOVIC Miraelina Prof. Faculty of Science, University of Ljubljana
OSORNIK Boris Assistant Plant Genetics and Breeding Department, Biotechnical Faculty Universit
BRANKA Javornik Assoc. Prof. Plant Genetics and Breeding Department, Biotechnical Faculty Univer
KREFT Ivan Prof, Plant Genetics and Breeding Department, Biotechnical Faculty University of
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1990: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1989: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1988: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | breeding barriers / biotechnology / embryo rescue / tissue culture / asexual gene transfer |
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| Research Abstract |
International collaboration between Miyazaki Univ., Faculty of Agric. And University of Ljubljana, Biotechnical Faculty has developed as these faculties became sister schools under the financial support entitled " Overcoming breeding barriers by means of plant biotechnology. Under the following categories some approaches have performed. 1) DNA transfer or genetic engineering, 2) chromosomal engineering for the introduction of alien genes, 3) Cellular handling including somatic fusion, 4) Clonal proliferation at tissue level.
Protoplasts were isolated from two apomictic species of Paspalum dilatatum and Panicum maximum. Protoplast division and colony formation were found to be effective. Attempts of protoplast fusion between both species were succeeded to form hybrid callus.
Preliminary work has been carried out on the tyrosine sulfation of protein in higher plants using callus. Tyrosylprotein sulfotransferase from the bovine liver Golgi was considered as a guide line for this study. No protein sulfation could be, however, observed in higher plants under the experimental conditions.
The chromosome association of amphidiploids of Iris laevigata x I. ensata were analysed and compared with that of the parental species and F_1 hybrids. These interspecific cross-breeding strategy was discussed.
To evaluate the yielding ability of buckwheat varieties, selections and crosses a new experimental approach was designed based on a comparison of the expected and actual yields of a certain plot.
Haploid plant have been induced by in vitro culture techniques in many species. This methods were applied to buckwheat genetics and breeding studies.
Isolation, identification and determination of glucosamine were performed in callus of potato. Accumulation of glucosamine was observed in aged-callus, and was particularly evident in the SDS-insoluble and soluble fractions. It is speculated that in aged-callus, structural components of the cell may be cemented by glucosamine.
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