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人工酵素

Research Project

Project/Area Number 63107002
Research Category

Grant-in-Aid for Special Project Research

Allocation TypeSingle-year Grants
Research InstitutionKyushu University

Principal Investigator

村上 幸人  九州大学, 工学部, 教授 (30037717)

Co-Investigator(Kenkyū-buntansha) 大野 惇吉  京都大学, 化学研究所, 助教授 (70027077)
西長 明  大阪工業大学, 工学部, 教授 (80025882)
広部 雅昭  東京大学, 薬学部, 教授 (20012594)
太垣 和一郎  大阪市立大学, 工学部, 教授 (30008502)
後藤 俊夫  名古屋大学, 農学部, 教授 (20023369)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥24,400,000 (Direct Cost: ¥24,400,000)
Fiscal Year 1988: ¥24,400,000 (Direct Cost: ¥24,400,000)
Keywords人工酵素 / オキシゲナーゼ活性 / フェレドキシンモデル / ブレオマイシン / 糖質変換 / 化学発光 / チトクロームP450モデル / 機能性ミセル
Research Abstract

適切なモデル系を用いることにより酵素の触媒機能の本質を見究めるとともに、構造的により安定で、かつ酵素が活性を示すことができない化学反応にも関与できる人工酵素の開発を研究目標としている。
(1)補酵素機能を規範とした高活性触媒の開発ーーオキシゲナーゼの反応中心モデルとしてコバルトシッフ塩基錯体を用いて新規合成反応を開拓した(西長)。フェレドキシンモデル錯体を用いて電気化学的二酸化炭素固定化に成功した(田中)。高活性フラビンモデルを用いて新しい酸化反応を見出した(矢野(由))。ブレオマイシン錯体のDNA塩基認識機能を解明すると共に、特異的塩基切断能をもつ人工ブレオマイシンの分子設計指針を確立した(杉浦)。Nー置換ジアミンの金属錯体を触媒として種々の糖質変換反応に成功した(矢野(重))。
(2)人工ホロ酵素の開発ーー機能性合成二分子膜をアポタンパクモデルとしてビタミンB_6及びB_<12>活性を発現する人工ホロ酵素を開発した(村上)。発光タンパク質であるエクオリンのモデル化と発光収率に及ぼす要因を明らかにするとともに、ミセル中でのウミホタルルシフェリン類縁体の化学発光に成功した(後藤)。安定性を向上させたチトクロームP450モデルを開発し、その触媒特性の要因を明らかにするとともに、薬物代謝研究への応用について展望を示した(広部)。ミセル系人工金属酵素を創製し、エステル交換、エステル加水分解に酵素類似の高活性を発現させることに成功した(太垣)。キノプロテイン酵素の補酵素であるピロロキノリンキノンをカチオン性ミセル内に固定化し、アミンオキシダーゼ様活性を発現させることに成功した(大城)。
(3)微生物酵素系の機能開発ーーNADPHが関与するパン酵母を用いて光学純度の高い生成物を得る還元反応を見出した(大野)。
以上3年間にわたる研究により所期の目標を達成することができた。

Report

(1 results)
  • 1988 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Yukito Murakami: Journal of the Chemical Society,Chemical Communications. 143-145 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yukito Murakami: Chemistry Letters. 621-624 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 村上幸人: 日本化学会誌. 445-451 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yukito Murakami: Journal of the Chemical Society,Chemical Communications. 856-858 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 村上幸人: 化学工業. 39. 948-954 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 村上幸人: 化学. 44. 68-69 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hideshi Nakamura: Bulletin of the Chemical Society of Japan. 61. 3776-3778 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Katsunori Teranishi: Bulletin of the Chemical Society of Japan.

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsunehisa Fujita: Bulletin of the Chemical Society of Japan. 61. 1661-1667 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsunehisa Fujita: Chemistry Letters. 981-984 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hiroshi Masumoto: Chemical and Pharmaceutical Bulletin. 37. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kazuhiro Aihara: Chemical and Pharmaceutical Bulletin. 36. 837-840 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 西長明: 日本化学会誌. 495-499 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Akira Nishinaga: Tetrahedron Letters. 29. 4115-4118 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Akira Nishinaga: Tetrahedron Letters. 29. 6309-6312 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Akira Nishinaga: Journal of Molecular Catalysis. 48. 249-264 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Akira Nishinaga: Tetrahedron Letters.

    • Related Report
      1988 Annual Research Report
  • [Publications] Akira Nishinaga: Tetrahedron Letters.

    • Related Report
      1988 Annual Research Report
  • [Publications] Atsuyoshi Ohno: Israel Journal of Chemistry. 28. 51-55 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Masayuki Fujii: Bulletin of the Chemical Society of Japan. 61. 495-500 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Atsuyoshi Ohno: Bulletin of the Chemical Society of Japan. 61. 1721-1724 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Atsuyoshi Ohno: Tetrahedron Letters. 29. 1951-1954 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kaoru Nakamura: Journal of Organic Chemistry. 53. 2589-2593 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Atsuyoshi Ohno: Tetrahedron Letters. 29. 3079-3082 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kaoru Nakamura: Bulletin of the Chemical Society of Japan. 61. 2089-2093 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kaoru Nakamura: Tetrahedron Letters. 29. 2453-2454 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kaoru Nakamura: Tetrahedron Letters. 29. 4769-4770 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Koji Tanaka: Bulletin of the Chemical Society of Japan. 61. 1285-1289 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Masami Nakamoto: Journal of the Chemical Society,Chemical Communications. 1422-1423 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Masami Nakamoto: Bulletin of the Chemical Society of Japan. 61. 4099-4105 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Koji Tanaka: Chemistry Letters. 2033-2036 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hitoshi Ishida: Inorganic Chemistry.

    • Related Report
      1988 Annual Research Report
  • [Publications] Koji Tanaka: Journal of the Chemical Society,Dalton Transactions.

    • Related Report
      1988 Annual Research Report
  • [Publications] Yumihiko Yano: Journal of Organic Chemistry. 52. 5606-5610 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kitaro Yoshida: Journal of the Chemical Society,Perkin Transactions 2. 2011-2013 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yumihiko Yano: Journal of Organic Chemistry.

    • Related Report
      1988 Annual Research Report
  • [Publications] Tomoaki Tanase: Chemistry Letters. 327-330 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tomoaki Tanase: Inorganic Chemistry. 27. 4085-4094 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shigenobu Yano: Coordination Chemisty Reviews. 92. 113-156 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 矢野重信: 日本化学会誌.

    • Related Report
      1988 Annual Research Report
  • [Publications] June Kuwahara: Proceedings of the National Academy of Sciences of the United States of America.85. 2459-2463 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 杉浦幸雄: 蛋白質・核酸・酵素. 33. 3102-3108 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsunehiko Higuchi: "The Role of Oxygen in Chemistry and Biochemistry" Elsevier Science Publishers B.V., 455-460 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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