• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the Exploitation of New Functions of Rice by Biotechnology

Research Project

Project/Area Number 63304011
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionKyoto University

Principal Investigator

YAMAGATA Hirotada  Kyoto Univ., Fac. of Agric., Professor, 農学部, 教授 (40026373)

Co-Investigator(Kenkyū-buntansha) SANO Yoshio  Natl. Inst. Genet., Associate Professor, 育種遺伝研究部門, 助教授 (70109528)
TANISAKA Takatoshi  Kyoto Univ., Fac. of Agric., Associate Professor, 農学部, 助教授 (80026591)
TSUNEWAKI Koichiro  Kyoto Univ., Fac. of Agric., Professor, 農学部, 教授 (20026438)
KINOSHITA Toshiro  Hokkaido Univ., Fac. of Agric., Professor, 農学部, 教授 (10001421)
TAKEDA Genkichi  Univ. of Tokyo, Fac. of Agric., Professor, 農学部, 教授 (90134501)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥17,300,000 (Direct Cost: ¥17,300,000)
Fiscal Year 1990: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1989: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1988: ¥6,200,000 (Direct Cost: ¥6,200,000)
KeywordsOryza sativa L. / Biotechnology / Micronucleus / Cell fusion / DNA / RFLP / Protein / Mutator / 遺伝子マ-カ- / 形質転換 / 遺伝子マーカー
Research Abstract

1. Establishment of new breeding techniques : Fusion between anther-derived haploid protoplasts successfully resulted in diploid plants. CO_2-gas supply to rice callus was effective for plant regeneration. Micronuclei were formed in suspension-culture cells by colchicine- and herbicide-treatments. A promoter gene enhancing the expression of reporter gene, necessary for efficient selection of transgenic plants, was found out. The physical map and clone bank of mitochondrial DNA in wild rices with B, C or E genome, were constructed. Nuclear DNA proved to have the sequences homologous with two small plasmidlike DNAs in mitochondria, B1 and B2. A herbicide (bialaphos) -resistant gene bar was successfully introduced into rice protoplast through electroporation. RFLP analysis revealed different direction of the linkage map with 1 and 2 linkage groups from that of old linkage map. New linkage map including isozyme-maker gene was constructed. The inheritance of slender-glume gene indispensable for activating mutator was clarified.
2. Exploitation of new function : RFLP's maker proved to be effective by used for detecting the genetical variation in anther-derived plants. There were found close relationships of endosperm proteins alpha -1 and alpha -3 with the diversification of genus Oryza. Waxy gene proved to have a mechanism determining Wx protein content. The amino acid compositin of prolamin polypeptides and the nucleotide sequence of cDNA encoding glutelin in rice seeds were clarified. A embryoless diploid mutant induced by gammaーirradiation to tetraploid rice was closely analyzed for inheritance and developmental physiology. Alien cytoplasm and fertility-restoring gene were introduced to common wheat and a new male sterility-fertility restoration system was developed for hybrid-wheat breeding.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (53 results)

All Other

All Publications (53 results)

  • [Publications] Hirai,A.: "Structure of cytoplasmic genomes in rice." Proceedings of 2nd Intern'l Rice Genet.symp.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kageyama,Y.: "The rice plants bearing endospermless mutants:A novel mutant induced by the tetraploidーirradiation technique in rice (Oryza sativa L.)." Japan.J.Breed.41. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kinoshita,T.: "Gene mapping of the first and second linkage groups in rice." J.Fac.Agr.Hokkaido Univ.64(3). 208-217 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kumamaru,T.: "Mutants for rice storage proteins of protein bodies in the starchy endosperm." Theoretical and Applied Genetics. 76. 11-16 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 宮崎 幸子: "作物における微小核の形成と単離" 育種学雑誌. 40(別2. 220-201 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oono,K.: "Current Status of Genetic Engineering for Plant Breeding." Proc.of the 6th Internal Congr.of SABRAO. 7-12 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oono,Y.: "Early flowering in transgenic tobacco plants possessing the rolC gene of Agrobacterium rhizogenes Ri poasmid." Jpn.J.Genet.65. 7-16 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oosumi,T.: "Analysis of glucose phosphate isomerase in nearーisogenic lines,and cultivar of rice (Oryza sativa L.)" Bot.Mag.Tokyo. 102. 283-289 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sakamoto,W.: "Analysis of mitochondrial DNAs from Oryza glaberrima and its cytoplasmic substituted line for Oryza sativa associated with cytoplasmic male" Jpn.J.Genet.65. 1-6 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sano,Y.: "The genetic nature of gamete eliminator in rice." Genetics. 125. 183-191 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takeuchi,Y.: "Molecular cloning and ethyline induction of mRNA encoding a phytoalexin elicitorーreleasing fact or,βー1,3ーendoglucanase,in soybean." Plant Physiology. 93. 673-682 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 谷坂 隆俊: "Mutator によってイネの量的形質に誘発される突然変異の課価." 近畿作育研究. 35. 79-86 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tsunewaki,K.: "Haploid production in wheat using the Salmon method." Bajaj,Y.P.S.(ed.)Biotechnology in Agriculture and Forestry. 13. 460-478 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 山縣 弘忠: "イネmutatorの育種的利用に関なる研究4.細粒遺伝子が体細胞分裂とPMCにおける染色体行動に及ぼす効果." 育種学雑誌. 39(別2. 394-395 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yomoda: "Effects of CO_2 gas supply on shoot regeneration in rice callus." Japan.J.Breed.41. 41-47 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hirai, A.: "Structure of cytoplasmic genomes in rice." Proceedings of 2nd Intern'I Rice Genet. Symp.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kageyama, Y.: "The rice plants bearing endospermless mutants : A novel mutant induced by the tetraploid-irradiation technique in rice (Oryza sativa L.)." Japan. J. Breed.41. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kinoshita, T.: "Gene mapping of the first and second linkage groups in rice." J. Fac. Agr. Hokkaido Univ.64(3). 208-217 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kumamaru, T.: "Mutants for rice storage proteins of protein bodies in the starchy endosperm." Theoretical and Applied Genetics. 76. 11-16 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miyazaki, S.: "Induction and isolation of micronuclei in some crop plants." Japan. J. Breed. 40(suppl. 2). 200-201 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oono, K.: "Current Status of Genetic Engineering for Plant Breeding." Proc. of the 6th Internal Congr. of Sabrao. 7-12 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oono, Y.: "Early flowering in transgenic tobacco plants possessing the rolC gene of Agrobacterium rhizogenes Ri poasmid." Jpn. J. Genet. 65. 7-16 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oosumi, T.: "Analysis of glucose phosphate isomerase in near-isogenic lines, and cultivar of rice (Oryza sativa L.)." Bot. Mag. Tokyo. 102. 283-289 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sakamoto, W.: "Analysis of mitochondrial DNAs from Oryza glaberrima and its cytoplasmic substituted line for Oryza sativa associated with cytoplasmic male sterility." Jpn. J. Genet. 65. 1-6 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sano, Y.: "The genetic nature of gamete eliminator in rice." Genetics. 125. 183-191 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takeuchi, Y.: "Molecular cloning and ethylene induction of mRNA encoding a phytoalexin elicitor-releasing fact or, beta-1, 3-endoglucanase, in soybean." Plant Physiology. 93. 673-682 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tanisaka, T.: "Evaluation of mutator-induced mutations for quantitative characters in rice." Rep. Soc. Crop Sci. Breed., Kinki. 35. 79-86 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tsunewaki, K.: "Haploid production in wheat using the Salmon method." Bajaj, Y. P. S. (ed.) Biotechnology in Agriculture and Forestry. 13. 460-478 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamagata, H.: "Studies on the utilization of mutator induced in rice. 4. Effect of a slender-glume gene on mitotic index and chromosome behavior in PMC." JApan. J. Breed.39(supp1.2). 394-395 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yomoda, A.: "Effects of CO_2 gas supply on shoot regeneration in rice callus." Japan. J. Breed. 41. 41-47 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hirai,A.: "Structure of cytoplasmic genomes in rice." Proceedings of 2nd Intern'l Rice Genet.Symp.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kageyama,Y.: "The rice plants bearing endospermless mutants :A novel mutant induced by the tetraploidーirradiation technique in rice (<Oryza>___ー <sativa>___ー L.)." Japan.J.Breed.41. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kinoshita,T.: "Gene mapping of the first and second linkage groups in rice." J.Fac.Agr.Hokkaido Univ.64(3). 208-217 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 宮崎 幸子: "作物における微小核の形成と単離." 育種学雑誌. 40(別2). 200-201 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Oono,Y.: "Early flowering in transgenic tobacco plants possessing the <rolC>___ー gene of <Agrobacterium>___ー <rhizogenes>___ー Ri poasmid." Jpn.J.Genet.65. 7-16 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sakamoto,W.: "Analysis of mitochondrial DNAs from <Oryza>___ー <glaberrima>___ー and its cytoplasmic substituted line for <Oryza>___ー <sativa>___ー associated with cytoplasmic male sterility." Jpn.J.Genet.65. 1-6 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sano,Y.: "The genetic nature of gamete eliminator in rice." Genetics. 125. 183-191 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takeuchi,Y.: "Molecular cloning and ethylene induction of mRNA encoding a phytoalexin elicitorーreleasing fact or,βー1,3ーendoglucanase,in soybean." Plant Physiology. 93. 673-682 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 谷坂 隆俊: "Mutatorによってイネの量的形質に誘発される突然変異の評価." 近畿作育研究. 35. 79-86 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tsunewaki,K.: "Haploid production in wheat using the Salmon method." Bajaj,Y.P.S.(ed.) Biotechnology in Agriculture and Forestry. 13. 460-478 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yomoda: "Effects of CO_2 gas supply on shoot regeneration in rice callus." Japan.J.Breed.41. 41-47 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] A.Kanno: "The nucleotide sequence and expression of the gene for the 32-KD quinone-binding protein from rice." Plant Science. 59. 95-99 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Nishizawa: "The nucleotide sequences and expression of genes for the beta and epsilon subunits of ATP synthase from rice." Japanese Journal of Genetics. 64. 223-229 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] R.Ishikawa: "Chromosomal analysis of isozyme loci and the allelic expression at cellular level in rice." J.Fac.Agr.Hokkaido Univ.64. 85-98 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] R.Matsuki: "Tissue specific expression of the rolC promotor of the Ri plasmid in transgenic rice plants." Mol.Gen.Genet.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Sano: "The directin of pollen flow between two co-occuring rice species,Oryza sativa and O.glaberrima." Heredity. 63. 353-357 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Tsunewaki: "Haploid production in wheat using the Salomon method." Biotecnology in Agriculture and Forestry. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Toriyama.: Biotechnology. 6. 1072-1074 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Toriyama.: Theor.Appl.Genet.76. 665-668 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] H.Oguro.: 育種学雑誌(Japan J.Breed). 38. 53-64 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Yamamoto.: 育種学雑誌(Japan J.Breed). 38. 470-473 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Nakamura.: Plant Sience. 56. (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 谷坂隆俊: 育種学雑誌. 38(別2). 294-295 (1988)

    • Related Report
      1988 Annual Research Report

URL: 

Published: 1988-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi