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Study on Infection and Multiplication of Densonucleosis Virus of Silkworm, Bombyx mori L.

Research Project

Project/Area Number 63304014
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 蚕糸学
Research InstitutionUtsunomiya University

Principal Investigator

IWASHITA Yoshimitsu  Utsunomiya University, Faculty of Agriculture, Professor, 農学部, 教授 (20007884)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Michihiro  Nagoya University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60111837)
NAKAGAKI Masao  Shinnsyu University, Faculty of Textile Science and Technology, Associate Profes, 繊維学部, 助教授 (70135169)
HUKUHARA Toshihiko  Tokyou University of Agriculture and Technology, Faculty of Agriculture, Profess, 農学部, 教授 (70011880)
WATANABE Hitoshi  (University of Tokyo, Faculty of Agriculture, Professor), 農学部, 教授 (10011868)
KAWASE Shigemi  (Nagoya University, Faculty of Agriculture, Professor), 農学部, 教授 (90023382)
管家 英治 (菅家 英治)  宇都宮大学, 農学部, 助教授 (20007967)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 1990: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1989: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1988: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsSericulture Science / Silkworm / Insect virus / Densonucleosis Virus (BmDNV) / Parvovirus / Viral nucleic acid / Base sequence of nucleic acid / Insect culture cell / 濃核病ウイルス(DNV) / 昆虫培養細胞 / 昆虫病理学 / 昆虫病理組織学 / ウイルス核酸の塩基配列 / ウイルス核酸のクロ-ニング / DNV / 昆虫の疫学 / ウイルス核酸のクローニング
Research Abstract

Studies on densonucleosis virus of silkworm, Bombyx mori, (BmDNV) which is divided into BmDNV-1, Ina-DNV, and BmDNV-2, Yamanashi-DNV (yDNV) and Chinese-DNV (cDNV), had been carried out for three years since 1988. The following results were obtained.
1. Dot-IGSS method was used in order to detect earlier infection with cDNV. The virus infection was detected ten hours after virus inoculation. The sensitivity showing positive reaction was high and 10ng.
2. In the mixed infection of cDNV and NPV or CPV, higher degree of interference between cDNV and NPV was shown.
3. Restriction enzyme mapping of the two DNAs, DNA-1 (6.56Kb) and DNV-2 (6.07Kb), of cDNV was tried. Some differences in base sequence between the two DNAs were found.
4. yDNV and cDNV are closely related viruses. Between the DNAs of the two DNVs, the nucleotide sequence of the terminal region was almost same. However, the restriction pattern of the cDNV DNA was different from that of the yDNV DNA.
5. Cloning of cDNV DNA-1 (6.56Kb) wa … More s tried. Genom DNA bearing the complete length was inserted into pUC119 plasmid vector and the making clone was succeeded.
6. Cloning of BmDNV-1 (4.9Kb) was carried out. By inserting genom DNA bearing the perfect length into pUC119 plasmid vector, clone was obtained. And by using clone p17238, all base sequence bearing 5048 nucleotides was analyzed.
7. cDNV had 5 structural proteins showing the molecular weight of 41, 43, 48, 51 and 100Kd, respectively, on SDS-PAGE gel. It was clarified that cDNV contained polyamine of spermidine, spermine and putrescine.
8. The complete sequence of the DNV DNA (5048 nucleotides) has inverted repeats of 225 nucleotides and the terminal 153 nucleotides are palindromic. The palindromes can fold back on themselves to form a hairpin structure but, unlike AAV, the small internal palidrome which forms a T-shaped conformation was observed.
9. Two insect cell lines were persistently infected ; one with isometric ssDNA virus 20nm in diameter and the other with an isometric ssRNA virus 22nm in diameter.
10. Iarvae of the mulberry pylarid, Glyphodes pyloalis, which infest mulberry plantations, are frequently infected in a chronic state with nonoccluded viruses that are serologically indistinguishable from the BmDNVs. The results suggest that the BmDNV originated from the lyphodes nonoccluded viruses, and epizootiologically, the mulberry pyralid is a common habitual host of these nonoccluded viruses. Less

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report

Research Products

(59 results)

All Other

All Publications (59 results)

  • [Publications] Abe,H.,M.Kobayashi,H.Watanabe: "Mosaic infction with a densonucleosis virs in the midgut epithelium of the silkworm,Bombyx mori." J.Invertebr.Pathol.55(1). 112-117 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Bando,H.,H.K.Choi,Y.Ito,S.Kawase: "Terminal structure of a densovirs implies a hairpin transfer replication which is similar to the model for AAV." Virology. 179. 57-63 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Choi,H.K.,T.Sasaki,T.Tomita,M.Kobayashi,S.Kawase: "Processing of structural polypeptides of infectious flacherie virus of the silkworm,Bombyx mori" VP1 and VP4 are derived from VP0.J.Invertebr.Patol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 福原 敏彦: "樹立細胞系における昆虫病原性ウイルスの増殖。" 組織培養. 5月号. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikeda,M.,T.Yaginuma,M.Kobayashi,O.Yamashita: "cDNA cloning,sequencing and temporal expression of the protease responsble for vitellin degradation in the silkworm,Bombyx mori." Comp.Biochem.Physiol.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 川瀬 茂実: "蚕の濃核病ウイルス。" 日蚕雑. 58(3). 295-301 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kobayashi,M.,Y.Hashimoto,H.Seki H.Watanabe: "In vitro trnslation of RNA from the midgut of the silkworm,Bombyx,mori,infected with a densonucleosis virus." J.Invertebr.Pathol.52. 259-267 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kobayashi,M.: "Selective diminishment of virusーspecific translatable mRNA in the densoncleosis virusーinfected midgut of the silkworm,Bombyx mori,reared at a supraoptimal temperature." J.Invertebr.Pathol.54. 379-384 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kobayashi,M.; H.K.Choi: "Inhibition of the accumulation of viral polypeptides in the densonucleosis virusーinfeced midgut of the silkworm,Bombyx mori,reared at a supraoptimal temperature." J.Invertebr.Pathol.56. 117-122 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Watanabe,H.;Y.Kurihara: "Comparative histopathology of two densonucleosis in the silkworm,Bombyx mori." J.Invertebr.Pathol.51(2). 287-290 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Watanabe,H.,Y.Kurihara,Y.‐X.Wang,T.Shimizu: "Mulberry pyralid,Glyhodes pyloalis : Habitual host of nonoccluded,viruses pathogen to the silkworm,Bombyx mori." J.Invertebr.Pathol.53(3). 401-408 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 陳 建宏・岩下 嘉光: "中陽細胞におけるウイルス混合感染に関する病理組織学的観察。" 日本蚕糸学会関東支部講演要旨集. 40. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hukuhara,T.;K.Yano: "The replicatio of small isometric viruses in estabrished lepidopteran cell lines." Vth internat.Colloq.Invertebr.Pathov.Microb.Control,. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 岩下 嘉光・鶴斑 裕治・難波 篤: "蚕の濃核病ウイルス(中国株)の核酸と蛋白質について。" 日本蚕糸学会講演要旨集. 58. 73- (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 岩下 嘉光: "消石灰液によるウイルス粒子の不活化。" 日本蚕糸学会講要旨集. 59. 55- (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 岩下 嘉光・南波 篤志: "消石灰液および高度サラシ粉液による濃核病ウイルスの不活化。" 日本蚕糸学会講演要旨集. 60. 74- (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 岩下 嘉光・安西 恵治: "消石灰液処理による中国株DNVの核酸と感染性。" 日本蚕糸学会講演要旨集. 61. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小林 迪弘・崔 洪圭: "濃核病ウイルス感染幼虫中腸におけるウイルスたんぱく質の高温による蓄積阻害。" 日本蚕糸学会東海支部講演要旨集. 41. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 中垣 雅雄・伴戸 久徳・伊藤 康彦・崔 洪圭・川瀬 茂実: "家蚕濃核病ウイルス(山梨株)の2種類の遺伝子のクロ-ニングて末端構造。" 日本蚕糸学会講演要旨集. 59. 54- (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 中垣 雅雄・伴戸 久徳・岩下 嘉光・川瀬 茂実: "家蚕に濃核病を引き起こす山梨株ウイルスと中国株ウイルスのゲノムの制限酵素消化からの比較。" 日本蚕糸学会中部支部講演要旨集. 45. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 中垣 雅雄・伴戸 久徳・岩下 嘉光・川瀬 茂実: "濃核病ウイルス山梨株と中国株のゲノムの比較。" 昆虫病理談話会会報. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 中垣 雅雄・長崎 仁・小池 靖夫・伴戸 久徳・石碑 路夫・川瀬 茂実・黒田 章夫・長島 栄一: "カイコ濃核病ウイルス(山梨株)のゲノムDNAの制限酵素地図。" 日本蚕糸学会中部支部講演要旨集. 44. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 中垣 雅雄・武井 隆三・長島 栄一・伴戸 久徳・岩下 嘉光・川瀬 茂実: "家蚕濃核病ウイルスのゲノムDNAの制限酵素地図。" 日本蚕糸学会講演要旨集. 60. 74- (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 中垣 雅雄・津田 英利・安武 宏高・何 彬・伴戸 久徳・岩下 嘉光・梶浦 善太・武井 隆三: "家蚕濃核病ウイルスのDNAの解析I。" 日本蚕糸学会中部支部講演要旨集. 46. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 中垣 雅雄・安武 宏高・津田 英利・何 彬・伴戸 久徳・岩下 嘉光・梶浦 善太・武井 隆三: "家蚕濃核病ウイルスのDNAの解析II。" 日本蚕糸学会中部支部講演要旨集. 46. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 中垣 雅雄・安武 宏高・津田 英利・伴戸 久徳・岩下 嘉光・川瀬 茂実・梶浦 善太・武井 隆三: "家蚕濃核病ウイルス(中国株)の遺伝子の末端構造。" 日本蚕糸学会講演要旨集. 61. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 外城 寿哉・小林 迪弘: "カイコ濃核病ウイルス(山梨株)たんぱく質の合成機構:感染中陽および糞におけるウイルス構成たんぱく質のイムノブロットによる検出。" 日本蚕糸学会東海支部講演要旨集. 42. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 外城 寿哉・小林 迪弘: "カイコ濃核病ウイルス(山梨株)構造たんぱく質の合成機構:感染中腸磨碎液のインキュベ-ションによるアイルスたんぱく質パタ-ンの変化。" 日本蚕糸学会講演要旨集. 61. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 渡部 仁・平山 力: "クワノメイガの保持するウイルスについて。" 日本応川動物学昆虫会講演要旨集. 33. 101- (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawase,S.,S.Garzon,D.M.Su,P.Tijssen: ""Insect parvovirus diseases" in P/ Tijssen ed.,CRC handbook of Parvoviruses.Vol.II," CRC Press,Boca Raton,U.S.A.,213-228 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawase,S.,E.Kurstak: ""Parvoviridae of invertbrates : Densonucleosis viruses" in E.Kurstak ed.,Invertebrate virses," Marcel Dekker,Inc.,New York,345-343 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tijssen,P.,M.Arella,S.Kawase: ""Molecular biology of densoncleosis virus" in P.Tijssen ed.,CRC handbook of parvoviruses.Vol.I," CRC Press,Boca Raton,U.S.A.,283-294 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Abe, H., M. Kobayashi and H. Watanabe: "Mosaic infection with a densonucleosis virus in the midgut epithelium of the silkworm, Bombyx mori." J. Invertebr. Pathol. 55(1),. 112-117 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Bando, H., H. K. Choi, Y. Ito and S. Kawase: "Terminal structure of a densovirus implies a hairpin transfer replication which is similar to the model for AAV." Virology. 179,. 57-63 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Choi, H. K., T. Sasaki, T. Tomita, M. Kobayashi and S. Kawase: "Processing of structural polypeptides of infectious flacherie virus of the silkworm, Bombyx mori : VP1 and VP4 are derived from VPO." J. Invertebr. Patol.,.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikeda, M., T. Yaginuma, M. Kobayashi and O. Yamashita: "oDNA cloning, sequencing and temporal expression of the protease responsible for vitellin degradation in the silkworm, Bombyx mori." Comp. Biochem. Physiol.,. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawase, s.: "Densonucleosis virus of the silkworm, Bombyx mori (In Japanese)" J. Seric. Sci. Jpn.58(4). 295-301 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kobayashi, M., Y. Hashimoto, H. Seki and H. Watanabe: "In vitro translation of RNA from the midgut of the silkworm, Bombyx mori, infected with a densonucleosis virus." J. Invertebr. Pathol.52. 259-267 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kobayashi, M.,: "Selective diminishment of virus-specific translatable mRNA in the densonucleosis virus-infected midgut of the silkworm, Bombyx mori, reared at a supraoptimal temperature." J. Invertebr. Pathol.54. 379-384 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kobayashi, M., and H. K Choi: "Inhibition of the accumulation of viral polypeptides in the densonucleosis virus-infected midgut of the silkworm, Bombyx mori, reared at a supraoptimal temperature." J. Invertebr. Pathol.,. 56. 117-122 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Watanabe, H., and Y. Kurihara: "Comparative histopathology of two densonucleosis in the silkworm, Bombyx mori." J. Invertebr. Pathol. 51(2). 287-290 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Watanabe, H., Y. Kurihara, Y. -X. Wang and T. Shimizu: "Mulberry pyralid, Glyhodes pyloalis : Habitual host of nonoccluded viruses pathogen to the silkworm, Bombyx mori." J. Invertebr. Pathol.52(3). 401-408 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawase, S., S. Garzon, D. M. Su and P. Tijssen: "Insect parvovirus diseases" CRC handbook of Parvoviruses.Vol. II. 213-228 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawase, S. and E. Kurstak: ""Parvoviridae of invertebrates : Densonucleosis viruses"" Invertebrate viruses. 315-343 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tijssen, P., M. Arella, and S. Kawase: ""Molecular biology of densonucleosis virus"" CRC handbook of parvoviruses. Vol. I. 283-294 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Abe,H.,M.Kobayashi,H.Watanabe: "Mosaic infection with a densonucleosis virus in the midgut epithelium of the silkworm,Bombyx mori." J.Invertebr.Pathol.55(1). 112-117 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Bando,H.,H.K.Choi,Y.Ito,S.Kawase: "Terminal structure of a densovirus implies a hairpin transfer replication which is similar to the model for AAV." Virology. 179. 57-63 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Choi,H.K.,T.Sasaki,T.Tomita,M.Kobayashi S.Kawase: "Processing of structural polypeptides of infectious flacherie virus of the silkworm,Bombyx mori" VP1 and VP4 are derived from VP0.J.Invertebr.Patol.

    • Related Report
      1990 Annual Research Report
  • [Publications] 福原 敏彦: "樹立細胞系における昆虫病原性ウイルスの増殖。" 組織培養. 5月号. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ikeda,M.,T.Yaginuma,M.Kobayashi O.Yamashita: "cDNA cloning,sequencing and temporal expression of the protease responsible for vitellin degradation in the silkworm,Bombyx mori." Comp.Biochem.Physiol.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 岩下嘉光: "人工飼料育蚕と桑葉育蚕における濃核病ウイルス(DNV)の感染増殖" 日本蚕糸学会関東支部学術講演要旨集. 40. 42 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 岩下嘉光: "消石灰液によるウイルス粒子の不活化" 日本蚕糸学会学術講演要旨集. 59. 55 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 渡部仁: "Mosaio infection with a Densonucleosis Vorus in the midgnt Epithelium of the Silkworm Bombyx mori." Journal of Inverterbrate pathology. 55. 112-117 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 中垣雅雄,川瀬茂実: "家蚕濃核病ウイルス(山梨株)の2種類の遺伝子のクロ-ニングと末端構造" 日本蚕糸学会学術講演要旨集. 59. 54 (1989)

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      1989 Annual Research Report
  • [Publications] 中垣雅雄,岩下嘉光,川瀬茂実: "家蚕に濃核病を引き起す山梨株ウイルスと中国株ウイルスのゲノムの制限酵素消化からの比較" 日本蚕系学会中部支部学術講演集. 45. 16 (1989)

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      1989 Annual Research Report
  • [Publications] 中垣雅雄,岩下嘉光,川瀬茂実: "家蚕濃核病ウイルスの山梨株と中国株ゲノムの比較" 昆虫病理談話会会報. 57. 9 (1990)

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      1989 Annual Research Report
  • [Publications] 伴戸久徳、伊藤康彦、中垣雅雄、崔洪圭、川瀬茂実: 日本蚕糸学会東海支部講演要旨集. 36. 22 (1988)

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      1988 Annual Research Report
  • [Publications] 伴戸久徳、伊藤康彦、崔洪圭、川瀬茂実: 日本蚕糸学会東海支部講演要旨集. 36. 23 (1988)

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      1988 Annual Research Report
  • [Publications] H.WATANABE.;Y.KURIHARA.;YU-XING WANG.: Journal of Invertebrate Pathology. 52. 401-408 (1988)

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      1988 Annual Research Report

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Published: 1988-03-31   Modified: 2016-04-21  

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