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Fundamental Studies on Serotyping and Genetic Recombination of Rotaviruses Isolated from Human and Animals.

Research Project

Project/Area Number 63304025
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

GOTO Hitoshi  Obihiro Uni. of Agri. & Vet. Med. Dept. of Vet. Microbiol. Professor, 畜産学部, 教授 (20003072)

Co-Investigator(Kenkyū-buntansha) HIRAGA Chikane  Saitama Med. School Dept. of Bacteriol. Assit. Professor, 講師 (30105910)
SHINAGAWA Morikazu  Obihiro Uni. of Agri. & Vet. Med. Dept. of Vet. Pub. Health Professor, 畜産学部, 教授 (00001537)
URASAWA Shozo  Sapporo Med. College Dept. of Hygiene & Epidemi. Professor, 教授 (00045379)
MINAMOTO Nobuyuki  Gifu Uni. Facul. of Agri. Dept. of Vet. Pub. Health Associ. Professor, 農学部, 助教授 (10144007)
OHTA Chikako  Obihiro Uni. of Agri. & Vet. Med. Dept. of Vet. Microbiol. Instructor, 畜産学部, 助手 (10176894)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥8,900,000 (Direct Cost: ¥8,900,000)
Fiscal Year 1989: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1988: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsAnimal origin / Rotavirus / Genetic structure / Serotyping / Pathogenicity
Research Abstract

Rotavirus (RV) has been recognized as a major causal agent of acute diarrhea in the youngs of human and animals. From the outer and inner capsid proteins, RV was identified as 5 groups, 2 subgroups, and 9 serotypes. The serotype 3 or 4 RV has been isolated from human and animals, and this fact is most important for the consideration of genetic recombinat vaccine for human and animals. The present study is concerned with distribution of RV in human and animals and the genetic structures of isolates. (1) Serotypes: The serotype 3 RV was isolated from fecal samples of foal diarrhea occurred in light horse farm. This is the first case of foal diarrhea due to serotype 3. The distribution of serotypes of human RV in diarrhea stools from Japan, Thailand and Bangladesh were predominant over serotypes 1 and 2. But its results showed relative frequency in locals and years. (2) Pathogenicity: Severe changes in intestine of gnotobiotic piglets inoculated with porcine RV were observed with diarrhea on 2-day-olds rather than 24-day-olds. Continuous administration of egg yolk or bovine colostral immunoglobulins prevented form RV diarrhea in a cat model of the disease or calf diarrhea in field case. (3) RV-RNA: VP6 of pigeon RV has the same antigenicity as turkey RV isolated in England. Two unique RV strains were isolated from human stools; one is the RV strains which belong to subgroup I but have "long" RNA pattern typical of subgroup II, and the other RV strains which has over 98% VP7 homology to the virulent human RV, since it has 90% VP4 homology to the RV.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] OHTA,Chikako: "Epizootiological and virological studies of foal diarrhea associated with serotype 3 rotavirus." Japanese Journal of Veterinary Science(Submit). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] FUKUSHO,Akio: "Serological characterization and polyacylamide gel electrophoresis of porcine retaviruses." Japanese Journal Veterinary Science(Submit). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MINAMOTO,Nobuyuki: "Characterization of monoclonai antibodies to VP6 of avian rotavirus which bear subgroup I specificity." Journal of Clinical Mirobiology(Subimt). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] TANIGUCHI,Koki: "Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus(strain K8)having unique VP4 neutralization epitopes." Journal of Virology. 63. 4101-4106 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KOBAYASHI,Nobumichi: "Unusal human rotavirus strains having subgroup I specificity and “long"RNA electropherotype." Archives of Virology. 109. 11-23 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] HIRAGA,Chikane: "Prevention of human rotsvirus infection with chicken egg yolk immunoglobulins containing rotavirus antibody in cat." Journal of Japanese Association for Infectious Diseases. 64. 118-123 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] OHTA, Chikako: "Epizootiological and virological studies of foal diarrhea associated with serotype 3 rotavirus." Japanese Journal of Veterinary Science (submit). (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] FUKUSHO, Akio: "Serological characterization and polyacylamide gel electrophoresis of porcine rotaviruses." Japanese Journal of Veterinary Science (submit) 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MINAMOTO, Nobuyuki: "Characterization of monoclonal antibodies to VP6 of avian rotavirus which bear subgroup I specificity." Journal of Clinical Microbiology (submit) 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] TANIGUCHI, Koki: "Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) having unique VP4 neutralization epitopes." Journal of Virology. 63. 1989 (101-4106)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] KOBAYASHI, Nobumichi: "Unusual human rotavirus strains having subgroup I specificity and "long" RNA electropherotype." Archives of Virology 109: 11-23, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] HIRAGA, Chikane: "Prevention of human rotavirus infection with chicken egg yolk immunoglobulins containing rotavirus antibody in cat." Journal of Japanese Association for Infectious Diseases 64: 118-123, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] GOTO,Hitoshi: "Prevention of rotavirus infection in newborn calves fed colostral immunoglobulins." Research in Veterinary Science. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] MINAMOTO,Nobuyuki: "Isolation and characterization of rotavirus from feral pigen in mammalian cell cultures" Epidemiology and Infection. 100. 481-492 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] URASAWA,Shozo: "Survey of human rotavirus serotype in defferent locals in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies." Journal of Infectious Deseases. 160. 44-51 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] PONGSUWANNE,Yaowapa: "Subgrou and serotypd distributions of human,bovine and porcine rotavirus in Thailns." Journal of Clinical Microbiology. 27. 1956-1960 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] AHMED,Muzahed Uddin: "Characterization of human rotavirus obained from diarrheic patients in Bangladesh by ELISA using subgroup and serotype specific monoclonal antibodies." Journal of Clinical Microbiology. 27. 1678-1681 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] URASAWA,Tomoko: Sapporo Medical Journal. 57. 373-378 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] TANIGUCHI,Koki: Journal of Virology. 62. 1870-1874 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] URASAWA,Shozo: Journal of Infectious Diseases.

    • Related Report
      1988 Annual Research Report
  • [Publications] OHTA,Chikako: Journal of Virology.

    • Related Report
      1988 Annual Research Report
  • [Publications] MINAMOTO,Nobuyuki: Avian Disease.

    • Related Report
      1988 Annual Research Report
  • [Publications] 平賀千兼: 実験動物.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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