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Microstructures and microdistributions of lymphatics.

Research Project

Project/Area Number 63304029
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionTOKYO MEDICAL COLLEGE

Principal Investigator

UCHINO Shigeo  TOKYO MED. COLL. MEDICAL DEP. PROFESSOR., 医学部, 教授 (00074500)

Co-Investigator(Kenkyū-buntansha) KATO Seiji  OITA MED. COLL. MEDICAL DEP. ASSISTANT PROFESSOR., 医学部, 助教授 (60034956)
MIYOSHI Masayuki  FUKUOKA UNIV. MEDICAL DEP. PROFESSOR., 医学部, 教授 (80077536)
MAGARI Sumiko  OSAKA MED. COLL. MEDICAL DEP. PROFESSOR., 医学部, 教授 (50084801)
FUJITA Tsuneo  NIIGATA UNIV. MEDICAL DEP. PROFESSOR., 医学部, 教授 (00032863)
KOTANI Masahiko  KUMAMOTO UNIV. MEDICAL DEP. PROFESSOR., 医学部, 教授 (20025521)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 1989: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1988: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsLymphatics / Monoclonal antibody against lymphatic endothelial cells / Lymphocyte infiltration / Immunoelectron microscopy / Lymph-reticular barrier / Enzyme histochemistry / Central lacteal / KOH-collagenase method / 起始リンパ管網 / ペプタイド含有神経 / リンパ管の微細構造 / リンパ管の微細分布 / リンパ管内皮特異的モノクローナル抗体 / リンパ浸潤 / リンパの輸送調節 / リンパ管の三次元的微細分布 / リンパ管の起始部
Research Abstract

1. A new double immunostaining technique using a monoclonal antibody (B27) in combination with a anti-type IV collagen antibody that stained basement membranes was developed to distinguish the lymphatic capillaries from the blood vessels.
2. Pancreatic islets of the NOD (non-obese diabetes) mouse observed by light and electron microscopy revealed severe lymphocyte infiltration, in which blood vessels and lymphatics are respectively disposed in appropriate positions as a supplying and a draining route for recirculating lymphocytes.
3. Distribution of nerves, especially neuropeptide-containing nerves and around lymphatics and bioactive substances in their endothelial cells, were examined by immunoelectron microscopy. Kinds of neuropeptide differ in the various tissues and organs.
4. The finding in the present study conclude that lymphocytes may migrate by their penetrating the cytoplasm of endothelial cells lining the lymphatic sinus.
5. On the basis of the characteristics of 5'-nucleotidase (5'-Nase) and alkaline phosphatase (ALPase), the differentiation of the lymphatics from blood capillaries was established on cryostat sections of several tissues using 57-Nase-ALPase double staining.
6. SEM of alkali-water macerated preparations showed the organization of collagen fibrils in the alimentary canal. Lymphatic vessels were housed in the collagen fibrillar sheaths. Those in the GALT had many fenestrations indicative of the sites for lymphocyte migration.
7. The intermediate portion of the central lacteal of the duodenal villus : Immediately beneath the endothelial lining of the lacteal there were a few bundles of fine unmyelinated nerve fibers. Some of these exhibited bulbar swellings which were in contact with or surrounded by the central lacteal endothelial cells.
8. The present study has demonstrated that the KOH-collagenase digestion method is useful for the SEM observation of the lymphatic system.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (82 results)

All Other

All Publications (82 results)

  • [Publications] 内野滋雄: "リンパ管の微細分布と微細構造" 脈管学. 29. 353-356 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kotani,M.: "The lymphatics and lymphoreticular tissues in relation to the action of sex hormones." Arch.Histol.Cytol.53. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Magari,S.: "An immunoelectromicroscopic study of von Willebrand factor in the thoracic duct endothelium of rats." Lymphology. 22. 76-80 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Magari,S.: "Hepatic lymphatic system." J.Gastroenterol.and Hepatol.5. 85-96 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Magari,S.: "Progress in basic lymphology:Fine structure and immunohistochemistry of the lymphatic system." International Congress series 88,Excepta medica.(1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyoshi,M.: "Contractile elements and innervation of the lymph sinus wall in the human appendix.A transmission electron microscopy."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato,S.: "Enzyme-histochemical visualization of lymphatic capillaries in the mouse tongue:Light and electron microscopic study." Okajimas Folia Anat.Jpn.65. 391-404 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 加藤征治: "酵素組織化学的方法によるリンパ管の確定法" リンパ学. 12. 13-21 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato,S.: "Enzyme-histochemical identification of lymphatic vessels by light and backscattered image scanning electron microscopy." Stain Technol.(1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato,S.: "Enzyme-histochemical demonstration of intralobular lymphatic vessels in the mouse thymus." Arch.Histol.Cytol.53. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato,S.: "Application of backscattered electron imaging to enzyme-histochemistry of lymphatic capillaries." J.Electron Microsc.(1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato,S.: "Enzymehistochemical analysis of 5'-nucleotidasepositive lymphatic endothelium." Acta Histochem.Cytochem.(1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohtani,O.: "Organization of the lymphatic vessels and their relationships to blood vessels in the rabbit Peyer's patches." Arch.Histol.Cytol.53. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 市川早苗: "ラット妊娠時の子宮内膜のリンパ管" 東医大誌. 47. 35-39 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa,S.: "Lymphatics of the cardiac chordae tendineae with particular consideration of their origin." Lymphology. 22. 123-131 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa,S.: "Nerve terminals associated with the central lacteal endothelial cells." Okajimas Folia Anat.Jpn.66. 57-60 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 市川早苗: "サル子宮内膜のリンパ管" リンパ学. 12. 23-31 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa,S.: "Intimate association of nerve terminals with the lacteal endothelium in the canine duodenal villi, Transmission microscopy of serial sections." Arch.Hict.Cytol.53. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ushiki,T.: "Three-dimensional organization and ultrastructure of lymphatics in the rat intestinal mucosa as revealed by scanning electron microscopy after KOH-collagenase treatment." Arch.Histol.Cytol.53. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Uchino,S.: "Endometrial blood vasculature and lymphatics of rats in oestrous cycle." Progress in Lymphology 11. 103-106 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Uchino,S.: "Lymphatic system of the pancreas." J.Cli.Exp.Med.144. 348-350 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Magari,S.: "Weibel-Palade bodies in endothelial cells of normal thoracic ducts and deep cervical lymphatics in rabbits" Lymphology. 21. 93-98 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato,S.: "Intralobular lymphatic vessels and their relationship to blood vessels in the mouse thymus." Cell Tissue Res.253. 181-187 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohtani,O.: "Scanning Electron Microscopy of Vascular Casts." Kluwer Academic Publishers.,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Uchino, S.: "Endometrial blood vasculature and lymphatics of rats in oestrous cycle." Progress in Lymphology. XI. 103-106 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Uchino, S.: "Lymphatic system of the pancreas." J. Cli. Exp. Med. 144 :348-350.1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Uchino, S.: "Microdistribution and microstructure of lymphatics." J. Jap. Coll. Aniol. 29 :353-356.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kotani, M.: "The lymphatics and lymphoreticular tissues in relation to the action of sex hormones." Arch. Histol. Cytol. 53: 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Magari, S.: "Weibel-Palade bodies in endothelial cells of normal thoracic ducts and deep cervical lymphatics in rabbits." Lymphology 21:93-98.1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Magari, S.: "An immunoelectronmicroscopic study of von Willebrand factor in the thoracic duct endothelium of rats." Lymphology 22:76-80.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Magari, S.: "Hepatic lymphatic system." J. Gastroenterol. and Hepatol. 5:85-96.1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Magari, S.: "Progress in basic lymphology: Fine structure and immunohistochemistry of the lymphatic system." Proceedings of XII th International Congress of Lymphology. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyoshi, M.: "Contractile elements and innervation of the lymph sinus wall in the human appendix. A transmission electron microscopy."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato, S.: "Intralobular lymphatic vessels and their relationship to blood vessels in the mouse thymus." Cell Tissue Res. 253 :181-187.1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato, S.: "Enzyme-histochemical visualization of lymphatic capillaries in the mouse tongue: Light and electron microscopic study." Okajimas Folia Anat. Jpn. 65 :391-404, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato, S.: "Enzyme-histochemmical identification of lymphatic vessels." Jap. Soci. Lymphology 12 :13-21, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato, S.: "Enzyme-histochemical identification of lymphatic vessels by light and backscattered image scanning electron microscopy." Stain Technol. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato, S.: "Enzyme-histochemical demonstration of intralobular lymphatic vessels in the mouse thymus." Arch. Histol. Cytol.53: 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato, S.: "Application of backscattered electron imaging to enzyme-histochemistry of lymphatic capillaries." J. Electron Microsc. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato, S.: "Enzymehistochemical analysis of 5 -nucleotidase positive lymphatic endothelium." Acta Histochem. Cytochem. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohtani, O.: "Organization of the lymphatic vessels and their relationships to blood vessels in the rabbit Peyer's patches." Arch. Histol. Cytol.53: 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohtani, O.: "Blood and lymphatic microvascular organization of the digestive tract. of Vascular Casts." Scanning Electron Microscopy Kluwer Academic Publishers.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa, S.: "Endometrial lymphatics of rats during pregnancy" J. Tokyo Med. Coll. 47 :35-39.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa, S.: "Lymphatics of the cardiac chordae tendineae with particular consideration of their origin." Lymphology 22:123-131.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa, S.: "Nerve terminals associated with the central lacteal endothelial cells." Okajimas Folia Anat. Jpn. 66 :57-60.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa, S.: "Lymphatic vessels of the endometrium of rhesus monkeys." Jap. Soci. Lymphology. 12 :23-31.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa, S.: "Intimate association of nerve terminals with the lacteal endothelium in the canine duodenal villi, Transmission microscopy of serial sections." Arch. Histol. Cytol.53: 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ushiki, T.: "Three-dimensional organization and ultrastructure of lymphatics in the rat intestinal mucosa as revealed by scanning electron microscopy after KOH-collagenase treatment." Arch. Histol. Cytol.53: 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 内野滋雄: "リンパ管の微細分布と微細構造" 脈管学. 29. 353-356 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kotani,M.: "The lymphatics and lymphoreticular tissues in relation to the action of sex hormones." Arch.Histol.Cytol.53. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Magari,S.: "An immunoelectronmicroscopic study of von Willebrand factor in the thoracic duct endothelium of rats." Lymphology. 22. 76-80 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Magari,S.: "Hepatic lymphatic system." J.Gastroenterol.and Hepatol.5. 85-96 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Magari,S.: "Progress in basic lymphology:Fine structure and immunohistochemistry of the lymphatic system." International Congress series 88,Excepta medica.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Miyoshi,M.: "Contractile elements and innervation of the lymph sinus wall in the human appendix.A transmission electron microscopy."

    • Related Report
      1989 Annual Research Report
  • [Publications] Kato,S.: "Enzyme-histochemical visualization of lymphatic capillaries in the mouse togue:Light and electron microscopic study." Okajimas Folia Anat.Jpn.65. 391-404 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 加藤征治: "酵素組織化学的方法によるリンパ管の確定法" リンパ学. 12. 13-21 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kato,S.: "Enzyme-histochemical identification of lymphatic vessels by light and backscattered image scanning electron microscopy." Stain Technol.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kato,S.: "Enzyme-histochemical demonstration of intralobular lymphatic vessels in the mouse thymus." Arch.Histol.Cytol.53. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kato,S.: "Application of backscattered electron imaging to enzyme-histochemistry of lymphatic capillaries." J.Electron Microsc.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kato,S.: "Enzymehistochemical analysis of 5'-uncleotidase positive lymphatic endothelium." Acta Histochem.Cytochem.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohtani,O.: "Organization of the lymphatic vessels and their relationships to blood vessels in the rabbit Peyer's patches." Arch.Histol.Cytol.53. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 市川早苗: "ラット妊娠時の子宮内膜のリンパ管" 東医大誌. 47. 35-39 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ichikawa,S.: "Lymphatics of the cardiac chordae tendineae with particular consideration of their origin." Lymphology. 22. 123-131 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ichikawa,S.: "Nerve terminals associated with the central lacteal endothelial cells." Okajimas Folia Anat.Jpn.66. 57-60 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 市川早苗: "サル子宮内膜のリンパ管" リンパ学. 12. 23-31 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ichikawa,S.: "Intimate association of nerve terminals with the lacteal endothelium in the canine duodenal villi,Transmission microscopy of serial sections." Arch.Hist.Cytol.53. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ushiki,T.: "Three-dimensional organization and ultrastructure of lymphatics in the rat intestinal mucoas as revealed by scanning electron microscopy after KOH-collagenase treatment." Arch.Histol.Cytol.53. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohtani,O.: "Scanning Electron Microscopy of Vascular Casts." Kluwer Academic Publishers.,

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Magari: Lymphology. 21. 93-98 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.Ito: Lymphology.

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Magari: Lymphology.

    • Related Report
      1988 Annual Research Report
  • [Publications] N.Inoue: Brain research.

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Uehars: Cel Tissue Research. 251. 547-553 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Miyoshi: Arch.Histol.Cytol.

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Ogawa: J.Ultrastr.Res.

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Kato: Okajimas Folia Anatomica Japonica. 65. (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] O.Ohtani: Arch.Histol.Cytol.

    • Related Report
      1988 Annual Research Report
  • [Publications] O.Ohtani: Arch.Histol.Cytol.

    • Related Report
      1988 Annual Research Report
  • [Publications] S.ichikawa: Lymphology.

    • Related Report
      1988 Annual Research Report
  • [Publications] 牛木辰男: 医生物走査電顕. 17. 73-75 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] H.Takahashi-Iwanaga: "Biopathology of the Liver, An Ultrastural Approach." Kluwer Academic Publishers., 59-68 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] H.Takahashi-Iwanaga: "P.M.Motta(ed.);Cells and Tissues, A Three-Dimensional Approach by Modern Techniques in Microscopy." Alan R,Liss, Inc., 10 (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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