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Molecular Diversity and Physiological Roles of Growth Inhibitors

Research Project

Project/Area Number 63304060
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 代謝生物化学
Research InstitutionYokohama City University (1989-1990)
Osaka University (1988)

Principal Investigator

MIYAZAKI Kaoru (1989-1990)  Yokohama City Univ., Kihara Inst. Biol. Res. ; Assoc. Prof., 木原生物学研究所, 助教授 (70112068)

堀尾 武一 (1988)  大阪大学, 蛋白質研究所, 教授 (70029932)

Co-Investigator(Kenkyū-buntansha) NISHIKAWA Katsuzo  Kanazawa Med. Univ. ; Prof., 教授 (10029960)
NATORI Shunji  Univ. Tokyo, Faculty of Pharmaceutical Sci. ; Prof., 薬学部, 教授 (50012662)
TANAKA Ryo  Nagoya City Univ., Faculty of Medicine ; Prof., 医学部, 教授 (90094383)
SUZUKI Fujio  Osaka Univ., Faculty of Dentistry ; Prof., 歯学部, 教授 (40028717)
IMANISHI Jiro  Kyoto Prefect. Univ. Med. ; Prof., 教授 (40112510)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 1990: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1989: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1988: ¥5,500,000 (Direct Cost: ¥5,500,000)
Keywordsgrowth inhibitors / antitumor agents / growth factors / cancer / mammalian cells / growth regulation / cell culture / purification / 増殖
Research Abstract

Considerable evidence has accumulated indicating that proliferation of animal cells is regulated by a balance between positive and negative growth stimuli. To clarify molecular species and physiological roles of growth inhibitors, each member of this research project has studied cytostatic or cytotoxic proteins present in different sources such as sera and cultured cells. Both cytostatic and cytotoxic factors were purified and characterized from normal rabbit serum. The former was a heat-and acid-labile but dithiothreitol-resistant protein with 56 kDa, which reversibly inhibited the growth of various malignant and nonmalignant cells. The latter existed in a latent form in the serum and was activated by incubating the serum at 37 ^OC for 12 h. Two active forms with 18 kDa and 36 kDa were purified from the incubated serum. Both proteins, which were heat- and acid-labile but dithiothreitol-resistant, exerted a strong cytolytic effect on rodent cells regardless of cell type and malignancy. Other growth inhibitors characterized in this study include mouse neonatal brain-derived carcinostatic factor (NBCF), tumor-degenerating factor (TDF) secreted by human fibroblasts, neuroblast growth inhibitory factor (NGIF) secreted by rat glioblasts, and tumor-regressing factor (TRF) induced by antitumor polysaccharides in the serum of tumor-bearing mice.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report

Research Products

(104 results)

All Other

All Publications (104 results)

  • [Publications] K.Miyazaki: "Potent growth inhition of human tumor cells in cuilture by arginine deiminase purified from culture medium of mycoplasmainfected cell line." Cancer Res.50. 4522-4527 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Miyazaki: "Purification and characterization of extracellular matrixーdegrading metalloーproteinase,matrin(pumpー1),secreted from human rectal carcinoma cell line." Cancer Res.50. 7758-7764 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] F.Umenishi: "Purificatin and properties of fibronectinーdegrading metalloproteinase secreted from Rous sarcoma virusーtransformed rat liver cell line RSVーBRL,and its identification as transin." J.Biochem.108. 537-543 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] F.Umenishi: "Efficient purification of TIMPー2 from culture medium conditioned by human hepatoma cell line,and its inhibitory effects on metalloproteinases end in vitro tumor invasios." J.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Takaku: "Characterization and in vivo antitumor activity of arginine deiminase purified from Mycoplasma arginini." Cancer Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Kimura: "Purification and properties of growth inhibitor from normal rabbit serm." J.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Kimura: "Purification and partial characterization of two types of growthinhibitory proteins latently present in rabbit serum." Biochim.Biophys.Acta.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 宮崎 香: "TGFーalphaとTGFーbetaの生理的役割" 臨床免疫ー特集 「Paracrine Aurocrine 増殖因子」. 22. 541-550 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 宮崎 香: "ウサギの血清中に存在する腫瘍細胞増殖阻害因子" Human Cell. 3. 118-123 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 宮崎 香: "細胞外マトリックスプロテア-ゼと癌の転移" 化学と生物. 28. 416-417 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 宮崎 香: "増殖阻害因子と細胞増殖のフィ-ドバック制御機構" 蛋白質核酸酵素臨時増刊「細胞増殖因子の基磯と臨床」共立出版. 121-128 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 山村 睦郎: "腫瘍変性因子(TDF)" Human Cell. 3. 113-117 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 国元 武彦: "腫瘍退縮因子" Humann Cell. 3. 124-130 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Takigawa: "Induction of angiogenesis in chick yolkーsac membrane by polyamines and its inhibition by tissues inhibitors of metalloproteinases(TIMP and TIMPー2)." Biochem.Biophys.Res.Commun.171. 1264-1271 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Takigawa: "Tumor angiogenesis and polyamines:alphaーdifluoromethylornithine,an irreversible inhibitor of ornithine decarboxylase,inhibits B16 melanimaーinduced angiogenesis in ovo and the proliferation of vascular endothelial cells in vitro." Cancer Res.50. 4131-4138 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] R.Kamijo: "Effects of combinations of transforming growth factor beta and TNF on inducthion of human myelogenous leukemic cell lines." J.lmmunol.144. 1311-1316 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.lwanami: "Combination effects of interferonーgamma and cholerona toxin on induction of differention of an insensitive Uー937 clone." Jpn.J.Cancer Res.81. 520-526 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] J.lto: "Cytoskeltal regulation of normal rat glioblasts differentiated by glia maturation factor." Neurochem.lnt.16. 133-140 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.AbeーDohmae: "Growth inhibition by sialosyl cholesterol of rat glioma cells." Neurochem.lnt.17. 93-100 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Kuzuhara: "Determination of of the disulfide array in sapecin,an antibacterial peptide of Sarcophaga peregrina(flesh fly)" J.Biochem.108. 128-132 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Yamada: "Production of recombinant sarcotoxin IA in Bombyx mori cells." Biochem.J.272. 633-636 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Matsuzaki: "Production of basic fibroblast growth factorーlike factor by cultured human cholangiocellular carcinoma cells." Jpn.J.Cancer Res.81. 345-354 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] N.Miwa: "Tumor growthーinhibtory glycoprotein secreted from mouse brain at the neonatal stage of the ontogenesis: molecular homogeneity and requirement of the protein conformation for exhibition of the cytotoxlc action." Biochim.Biophys.Acta. 1034. 309-317 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Kageyama: "Enhanced inhibition of DNA synthesis and release of membrane phospholipid in tumor cells treated with a combination of acylated ascorbate and hyperthermia." lnt.J.Hyperthermia. 7. 85-91 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 今西 二郎: "新しいサイトカイン" 金芳堂, 171 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Miyazaki: "Potent growth inhibition of human tumor cells in culture by arginine deiminase purified from culture medium of mycoplasma-infected cell line." Cancer Res.50. 4522-4577 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Miyazaki: "Purification and characterization of extracellular matrix-degrading metallo-proteinase, matrin (pump-1), secreted from human rectal carcinoma cell line." Cancer Res. 50. 7758-7764 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] F. Umenishi: "Purification and properties of fibronectindegrading metallo-proteinase secreted from Rous sarcoma virus-transformed rat liver cell line RSV-BRL, and its identification as transin." J. Biochem. 108. 537-543 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] F. Umenishi: "Efficient purification of TIMP-2 from culture medium conditioned by human hepatoma cell line, and its inhibitory effects of metallo- proteinases and in vitro tumor invasion." J. Biochem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kimura: "Purification and properties of growth inhibitor from normal rabbit serum." J. Biochem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Takigawa: "Induction of angiogenesis in chick yolk-sac membrane by polyamines and its inhibition by tissues inhibitors of metalloproteinases (TIMP and TIMP-2)" Biochem. Biophys. Res. Commn.171. 1264-1271 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Takigawa: "Tumor angiogenesis and polyamines : alpha-difluoro-methylornithine, an irreversible inhibitor of ornithine decarboxylase, inhibits B16 melanoma-induced angiogenesis in vivo and the proliferation of vascular endothelial cells in vitro." Cancer Res.50. 4131-4138 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] R. Kamijo: "Effects of combinations of transforming growth factor beta and TNF on induction of human myelogenous leukemic cell lines." J. Immunol.144. 1311-1316 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Iwamami: "Combination effects of interferon-gamma and cholera toxin on induction of differentiation of an insensitive U-937 clone." Jpn. J. Cancer Res.81. 520-526, (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] J. Ito: "Cytoskeletal regulation of normal rat glioblasts differentiated by glia maturation factor." Neurochem. Int.16. 133-140 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Abe-Dohmae: "Growth inhibition by sialosyl cholesterol of rat glioma cells." Neurochem. Int.17. 93-100 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kuzuhara: "Determination of the disulfide array in sapecin, an antibacterial peptide of Sarcophaga peregrina (flesh fly)" J. Biochem.108. 128-132 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Yamada: "Production of recombinant sarcotoxin IA in Bombyx mori cells." Biochem. J. 272. 633-636 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Matsuzaki: "Production of basic fibroblast growth factor-like factor by cultured human cholangiocellular carcinoma cells." Jpn. J. Cancer Res.81. 345-354 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] N. Miwa: "Tumor growth-inhibitory glycoprotein secreted from mouse brain at the neonatal stage of the ontogenesis : molecular homogeneity and requirement of the protein conformation for exhibition of the cytotoxic action." Biochem. Biophys. Acta. 1034. 309-317 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Kageyama: "Enhanced inhibition of DNA synthesis and release of membrane phospho-lipid in tumor cells treated withe a combination of acylated ascorbate and hyperthermia." Int. J. Hyperthermia. 7. 85-91 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Miyazaki: "Potent growth inhibition of human tumor cells in cuilture by arginine deiminase purified from culture medium of mycoplasmaーinfected cell line." Cancer Res.50. 4522-4527 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Miyazaki: "Purification and characterization of extracellular matrixーdegrading metalloーproteinase,matrin(pumpー1),secreted from human rectal carcinoma cell line." Cancer Res.50. 7758-7764 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] F.Umenishi: "Purification and properties of fibronectinーdegrading metalloーproteinase secreted from Rous sarcoma virusーtransformed rat liver cell line RSVーBRL,and its identification as transin." J.Biochem.108. 537-543 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] F.Umenishi: "Efficient purification of TIMPー2 fーrom culture medium conditioned by human hepatoma cell line,and its inhibitory effects on metalloーproteinases and <in vitro>___ー tumor invasion." J.Biochem.

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Takaku: "Characterization and <in vivo>___ー antitumor activity of arginine deiminase purified from <Mycoplasma arginini>___ー." Cancer Res.

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kimura: "Purification and properties of growth inhibtor from normal rabbit serum." J.Biochem.

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kimura: "Purification and partial characterization of two types of growthーinhibitory proteins latently present in rabbit serum." Biochim.Biophys.Acta.

    • Related Report
      1990 Annual Research Report
  • [Publications] 宮崎 香: "TGFーαとTGFーβの生理的役割" 臨床免疫ー特集「Paracrine Aurocrine増殖因子」. 22. 541-550 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 宮崎 香: "ウサギの血清中に存在する腫瘍細胞増殖阻害因子" Human Cell. 3. 118-123 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 宮崎 香: "細胞外マトリックスプロテア-ゼと癌の転移" 化学と生物. 28. 416-417 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 宮崎 香: "増殖阻害因子と細胞増殖のフィ-ドバック制御機構" 蛋白質核酸酵素臨時増刊「細胞増殖因子の基礎と臨床」共立出版. 121-128 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 山村 睦郎: "腫瘍変性因子(TDF)" Human Cell. 3. 113-117 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 国元 武彦: "腫瘍退縮因子" Humann Cell. 3. 124-130 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Takigawa: "Induction of angiogenesis in chick yolkーsac membrane by polyamines and its inhibition by tissues inhibitors of metalloproteinases(TIMP and TIMPー2)." Biochem.Biophys.Res.Commun.171. 1264-1271 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Takigawa: "Tumor angiogenesis and polyamines:αーdifluoromethylornithine,an irreversible inhibitor of ornithine decarboxylase,inhibits B16 melanomaーinduced angiogenesis <in ovo>___ー and the proliferation of vascular endothelial cells <in vitro>___ー." Cancer Res.50. 4131-4138 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] R.Kamijo: "Effects of combinations of transforming growth factor β and TNF on induction of human myelogenous leukemic cell lines." J.Immunol.144. 1311-1316 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Iwanami: "Combination effects of interferonーγ and cholera toxin on induction of differentiation of an insensitive Uー937 clone." Jpn.J.Cancer Res.81. 520-526 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Ito: "Cytoskeletal regulation of normal rat glioblasts differentiated by glia maturation factor." Neurochem.Int.16. 133-140 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.AbeーDohmae: "Growth inhibition by sialosyl cholesterol of rat glioma cells." Neurochem.Int.17. 93-100 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kuzuhara: "Determination of of the disulfide array in sapecin,an antibacterial peptide of <Sarcophaga peregrina>___ー(flesh fly)" J.Biochem.108. 128-132 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Yamada: "Production of recombinant sarcotoxin IA in <Bombyx mori>___ー cells." Biochem.J.272. 633-636 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Matsuzaki: "Production of basic fibroblast growth factorーlike factor by cultured human cholangiocellular carcinoma cells." Jpn.J.Cancer Res.81. 345-354 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] N.Miwa: "Tumor growthーinhibtory glycoprotein secreted from mouse brain at the neonatal stage of the ontogenesis:molecular homogeneity and requirement of the protein conformation for exhibition of the cytotoxic action." Biochim.Biophys.Acta. 1034. 309-317 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Kageyama: "Enhanced inhibition of DNA synthesis and release of membrane phosphoーlipid in tumor cells treated with a combination of acylated ascorbate and hyperthermia." Int.J.Hyperthermia. 7. 85-91 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 今西 二郎: "新しいサイトカイン" 金芳堂, 171 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Miyazaki: "Growth inhibitors-molecular diversity and roles in cell proliferation." In Vitro Cell.Develop.Biol.25. 866-872 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Miyazaki: "Characterization of fibronectin-hydrolyzing metallo-proteinase secreted from virally transformed rat liver cell line RSV-BRL." Intracellular Protein Catabolism Japan Scientific Societies Press,Tokyo. 559-565 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Miyazaki: "Potent growth inhibition of human tumor cells in cuilture by arginine deiminase purified from culture medium of mycoplasma-infected cell line." Cancer Res.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 宮崎香: "ウサギの血清中に存在する腫瘍細胞増殖阻害因子" Human Cell. 3. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Okumura-Noji: "Inhibition by dipheny lhydation of growth and morphological differentiation of glial cells;implication of calcium." Neurochem.Int.14. 353-359 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Ohsawa: "Expression of tumor necrosis factor at a specific developmental stage of mouse embryo." Devel.Biol.135. 459-461 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 今西芳二郎: "ヒト腫瘍変性因子(TDF)" 松仁会誌. 28. 1-7 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Takigawa: "Establishment from mouse growth cartilage of clonal cell lines with responsiveness to parathyroid hormone,alkaline phosphatase activity,and an ability to produce an endothelial cell growth inhibitor." Calcif.Tissue.Int.45. 305-313 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Takigawa: "Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes." Cancer Res.49. 3996-4002 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] R.Kamijo: "Effects of combinations of transforming growth factor-β1 and TNF on induction of differentiation of human myelogenous leukemic cell lines." J.Immunol.

    • Related Report
      1989 Annual Research Report
  • [Publications] R.Kamijo: "Suppression of TNF-stimulated proliferation of diploid fibroblasts and TNF-induced cytotoxicity against transformed fibroblasts by TGF-β1." Biochem.Biophys.Res.Commun.15. 98-101 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] J.Ito: "Induction of astroglial growth inhibition and differentiation by sialosyl cholesterol." Brain Res.4818. 335-343 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Kurata: "Dissociation of Sarcophaga peregrina(flesh fly) fat body by pupal hemocytes in vitro" J.Insect.Physiol.35. 559-565 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Tanaka: "Identification of a family of insulin-like growth factor II secreted by cultured rat epithelial-like cell line 18,54-SF." Endocrinology. 124. 870-877 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Matszaki: "Monoclonal antibodies against heparin-binding growth factor II/basic fibroblast growth factor that block its biological activity:invalidity of the antibodies for tumor angiogenesis." Proc.Natl.Acad.Sci.USA. 86. 9911-9915 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Miwa: "Carcinostatic substances secreted from that animal brain." Reports Res.Meat Products. 7. 483-490 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Miwa: "Tumor growth-inhibitory glycoprotein secreted from mouse brain at the neonatal stage of the ontogenesis:molecular homogeneity and requirement of the protein conformation for exhibition of the cytostatic action." Biochim.Biophys.Acta.

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Hanai: "Central action of acidic fibroblast growth factor in feeding regulation." Am.J.Physiol.256. 217-223 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 宮崎香: "上皮細胞「無血清細胞マニュアル」" 講談社, 248( P89-104) (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 今西二郎: "新しいサイトカイン" 金芳堂, 171 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Mashima,: J.Biochem.103. 1020-1026 (1988)

    • Related Report
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  • [Publications] 宮崎香: Biotherapy. 2. 285-291 (1988)

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  • [Publications] K.Miyazaki,: In Vitro. (1989)

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  • [Publications] 今西二郎: Biotherapy. 2. 299-304 (1988)

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  • [Publications] 国元武彦: Biotherapy. 2. 292-298 (1988)

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  • [Publications] 鈴木不二男: Biotherapy. 2. 278-284 (1988)

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  • [Publications] K.Takeda,: Biochem.Biophys.Res.Commun.155. 24-31 (1988)

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  • [Publications] R.Kamijo,: Biochem.Biophys.Res.Commun.158. 155-162 (1989)

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  • [Publications] S.Aono,: J.Neurochem.50. 700-703 (1988)

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  • [Publications] T.Kato,: Brain Res.438. 277-285 (1988)

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  • [Publications] J.Ito,: Brain Res.481. 335-343 (1989)

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  • [Publications] K.Ando,: Biochemistry. 27. 1715-1721 (1988)

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  • [Publications] K.Matsuyama,: J.Biol.Chem.263. 17112-17116 (1988)

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  • [Publications] K.Matsuyama,: J.Biol. Chem.263. 17117-17121 (1988)

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  • [Publications] 三羽信比古: Biotherapy. 2. 311-317 (1988)

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  • [Publications] 三羽信比古: 生化学. 60. 973 (1988)

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  • [Publications] Y.Yoshitake,: Arch.Biochem.Biophys.263. 437-446 (1988)

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  • [Publications] Y.Masuda,: In Vitro Cell.Dev.Biol.24. 893-899 (1988)

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Published: 1988-03-31   Modified: 2016-04-21  

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