| Project/Area Number |
63304060
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
代謝生物化学
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| Research Institution | Yokohama City University (1989-1990)
Osaka University (1988) |
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Principal Investigator |
MIYAZAKI Kaoru (1989-1990) Yokohama City Univ., Kihara Inst. Biol. Res. ; Assoc. Prof., 木原生物学研究所, 助教授 (70112068)
堀尾 武一 (1988) 大阪大学, 蛋白質研究所, 教授 (70029932)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIKAWA Katsuzo Kanazawa Med. Univ. ; Prof., 教授 (10029960)
NATORI Shunji Univ. Tokyo, Faculty of Pharmaceutical Sci. ; Prof., 薬学部, 教授 (50012662)
TANAKA Ryo Nagoya City Univ., Faculty of Medicine ; Prof., 医学部, 教授 (90094383)
SUZUKI Fujio Osaka Univ., Faculty of Dentistry ; Prof., 歯学部, 教授 (40028717)
IMANISHI Jiro Kyoto Prefect. Univ. Med. ; Prof., 教授 (40112510)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 1990: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1989: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1988: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | growth inhibitors / antitumor agents / growth factors / cancer / mammalian cells / growth regulation / cell culture / purification / 増殖 |
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| Research Abstract |
Considerable evidence has accumulated indicating that proliferation of animal cells is regulated by a balance between positive and negative growth stimuli. To clarify molecular species and physiological roles of growth inhibitors, each member of this research project has studied cytostatic or cytotoxic proteins present in different sources such as sera and cultured cells. Both cytostatic and cytotoxic factors were purified and characterized from normal rabbit serum. The former was a heat-and acid-labile but dithiothreitol-resistant protein with 56 kDa, which reversibly inhibited the growth of various malignant and nonmalignant cells. The latter existed in a latent form in the serum and was activated by incubating the serum at 37 ^OC for 12 h. Two active forms with 18 kDa and 36 kDa were purified from the incubated serum. Both proteins, which were heat- and acid-labile but dithiothreitol-resistant, exerted a strong cytolytic effect on rodent cells regardless of cell type and malignancy. Other growth inhibitors characterized in this study include mouse neonatal brain-derived carcinostatic factor (NBCF), tumor-degenerating factor (TDF) secreted by human fibroblasts, neuroblast growth inhibitory factor (NGIF) secreted by rat glioblasts, and tumor-regressing factor (TRF) induced by antitumor polysaccharides in the serum of tumor-bearing mice.
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