| Project/Area Number |
63430022
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Faculty of Pharmaceutical Sciences, University of Tokyo |
|---|
Principal Investigator |
ARATA Yoji Faculty of Pharmaceutical Sciences University of Tokyo, Professor, 薬学部, 教授 (40011499)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Yoshifumi Yokohama City University Graduate School of Integrated Science, Professor, 大学院総合理学研究科, 教授 (70107390)
|
|---|
| Project Period (FY) |
1988 – 1989
|
| Project Status |
Completed (Fiscal Year 1989)
|
| Budget Amount *help |
¥29,600,000 (Direct Cost: ¥29,600,000)
Fiscal Year 1989: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1988: ¥24,500,000 (Direct Cost: ¥24,500,000)
|
|---|
| Keywords | NMR / Immunoglobulin / Antigen-antibody interactions |
|---|
| Research Abstract |
The aim of this research is 1) to understand the molecular struetural basis of the antigen recognition by antibody and 2) to develop a general means of providing antibody with a variety of reactivity on the basis of the information obtained. Two-dimensional homonuclear Hartmann-Hahn (2D-HOHAHA) spectroscopy was found to be effective in establishing the connectivity between the C2-H and C4-H protons of His residues in the antibody molecule. The antibody molecule was selectively labeled with ^<13>C at the carbonyl carbon of a variety of amino acid residues including methionine and tryptophan by growing hybridoma cells in serum-free medium. It was possible to observe all the carbonyl carbon resonances of the antibody. On the basis of the results obtained using the technique of stable isotope labeling, structure-function correlates for the antibody molecule have successfully been discussed.
|
