• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Research for elucidating the mechanism of ischemia-reperfusion injury at cardiopulumonary bypass, and taking countermeasures against it (1990)

Research Project

Project/Area Number 63440052
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionKeio University

Principal Investigator

INOUE Tadashi  Keio University; Surgery; Professor, 医学部外科, 教授 (30050991)

Co-Investigator(Kenkyū-buntansha) ISEKI Harukazu  Keio University; Surgery; Assistant, 医学部外科, 助手 (00212960)
SUZUKI Takaaki  Keio University; Surgery; Assistant, 医学部外科, 助手 (10196834)
NAGUMO Masashi  Keio University; Surgery; Assistant, 医学部外科, 助手 (70198365)
YOZU Ryohei  Keio University; Surgery; Assistant, 医学部外科, 講師 (30129738)
SOMA Yasuhiro  Keio University; Surgery; Lecturer, 医学部外科, 講師 (40051437)
三角 隆彦  慶應義塾大学医学部, 外科, 助手 (20174013)
申 範圭  慶應義塾大学医学部, 外科, 助手 (50171062)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1989: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1988: ¥4,700,000 (Direct Cost: ¥4,700,000)
KeywordsIschemic myocardium / Reperfusion injury / Polymorphonuclear leukocytes / Free radical / Endothelial cell / 微小循環 / 心筋再灌流障害 / 多核白血球 / ケミルミネッセンス法 / TBA法
Research Abstract

Recently the reperfusion injury, especially by oxygen-derived free radicals, has been noticed in cardiac surgery. But the mechanism has not been clarified. As one of sources of reperfusion injury, we noticed respiratory burst of polymorphonuclear leukocytes (PMNs), which produce a lot of free radicals. At first we looked over the change of chemiluminescence (ChL) activity in PMNs during cardiopulmonary bypass to investigate whether respiratory burst of PMNs would be apt to occur. The activity increased 1.82 times (p<.05) 30 min. after reperfusion. This result pointed out that the respiratory burst of PMNs would be liable to occur after reperfusion, namely it suggested that PMNs would take a part in reperfusion injury. Next we investigated whether leukocyte depletion of reperfuming blood would reduce the degree of reperfusion injury of not under the canine cross circulation method. The group reperfused with leukocyte depleted blood showed better recovery of left ventricle function, less peroxidation products, and less CPK-MB release. Pathologically, electron micrograph showed more extracellular edema, much platblete adhesion on the endothelium, much endothelial cell damage, and more mitochondrial damage in this group. In contrast to this group, in the group reperfused with whole blood less endothelial and mitochondrial damage. Further we tried to examine endothelial and mitochondrial damage in the group in administration of superoxide dismutase, as a free radical scavenger, during heart arrest. This group showed much the same endothelial and mitochondrial damage as the whole blood group. These results suggest that PMNs would take some parts in reperfusion injury, and free radical derived endothelial damage just after reperfusion would occur as the trigger of the foll owing PMNs derived reperfusion injury. After this, the endothelial change after reperfusion will be required on closer investigation.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 井関治和: "虚血心筋再灌流傷害の機序解明についての検討ー活性酸素による微小循環傷害とその対策ー" 慶應医学. 67. 185-198 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 井関治和: "体外循環下手術における多形核白血球活性変化" 臨床胸部外科. 10. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Harukazu Iseki: "INVESTIGATION FOR THE MECHANISM OF REPERFUSION INJURY IN ISCHEMIC MYOCARDIUM" KEIO JOURNAL OF MEDICINE. 67. 185-198 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 井関,治和: "虚血心筋再灌流傷害の機序解明についての検討" 慶應医学. 61. 185-198 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 井関,治和: "体外循環不可術における多形核白血球活性変化" 臨床胸部外科. 10. (1990)

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1988-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi