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Induction and Identification of Endogenous Anti-Ischemic Mechanisms (Factors)

Research Project

Project/Area Number 63440058
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionNigata University

Principal Investigator

SHIMOJI Koki  Niigata University School of Medicine, Professor, 医学部, 教授 (30040158)

Co-Investigator(Kenkyū-buntansha) DENDA Sadahei  Niigata University School of Medicine, Assistant, 医学部, 助手 (20197833)
TAGA Kiichiro  Niigata University Hospital, Assistant, 医学部附属病院, 助手 (00163329)
FUJIWARA Naoshi  Niigata University Hospital, lecturer, 医学部附属病院, 講師 (70181419)
ENDOH Hiroshi  Niigata University Hospital, Lecturer, 医学部附属病院, 講師 (90168831)
FUKUDA Satoru  Niigata University School of Medicine, Assistant Professor, 医学部, 助教授 (30116751)
冨田 美佐緒  新潟大学, 医学部附属病院, 助手 (60221438)
熊谷 雄一  新潟大学, 医学部附属病院, 助手 (90170050)
穂苅 環  新潟大学, 医学部附属病院, 助手
大浜 栄作  新潟大学, 脳研究所, 助教授 (50018892)
Project Period (FY) 1988 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1988: ¥9,000,000 (Direct Cost: ¥9,000,000)
KeywordsBrain Injury / Anti-Ischemic Activity / Intracellular Free Calcium / Nerve Growth Factor / Regional Metabolic Activity / 海馬切片 / 細胞内Ca^<2+> / 低酸素・無グルコ-ス / 脳保護 / 脳虚血 / 細胞内遊離カルシウムイオン / ^<14>Cーデオキシグルコ-ス / 局所脳代謝 / 機械的脳損傷 / 抗脳虚血作用 / 体温 / 脳保護作用 / 局麻痙攣 / 神経生成因子
Research Abstract

We have previously suggested that artificial minor brain injury induces certain endogenous anti-ischemic mechanisms in the mouse brain. In this study, we examined the survival rate of mice following brain ischemia and characterized the endogenous anti-ischemic activities induced by minor brain injury. The survival rates following incomplete brain ischemia was improved during 1 to 2 weeks following artificial brain injury in the mice. Artificial brain injury also improved the survival from the lidocain-induced seizure. Post-ischemic increase in regional metabolic activity was attenuated in the injured mouse brain. The direct injection of nerve growth factor (NGF), one of neurotropic factors, into the brain tissue did not improve the survival rate of the mice following brain ischemia. On the other hand, we found that Ca^<2+> overload in cytosol may relate to irreversible dysfunction of neurons in hippocampal slices exposed to hypoxia and the increase in intracellular free Ca^<2+> concentration induced by hypoxia in the absence of glucose was attenuated in the vicinity of lesion site in hippocampal CA1 region of mouse brain slices. The results suggest that the anti-ischemic mechanisms may be activated during 1 to 2 weeks in the recovering process from brain injury and may be protective against lidocaine-induced seizure and hypermetabolism following brain ischemia. The origin of anti-ischemic activity may be not assigned to NGF, but certain factor (s) may be produced in the lesion site to attenuate the increase in intracellular free Ca^<2+> concentration, protecting the ischemic cell damage.

Report

(5 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 高畑 与四夫ら: "軽度脳損傷後の抗虚血作用、ー永久結紮モデルおよびマウス亜系(型)の影響ー" 蘇生. 6. 92 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤原 直士ら: "脳虚血マウスの生存率に及ぼす神経成長因子(NGF)の影響" 麻酔. 38. S174 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Koki Shimoji,et al.: "Protective effect of microinjury in brain ischemic demage" Advances in Brain Resuscitaiton. 115-121 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 下地 恒毅ら: "脳組織切片を用いた脳虚血実験法" 病態生理. 10. 343-350 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi Abe et al.: "Simultaneous recording of 〔Ca2+〕i and population potential in the fippocampal slice during hypoxia" Neurosci.Res.(Supplment). 14. S19 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Naoshi Fujuwara et al.: "Changes in intrecellular pH of mouse hippocampal slices induced by high K^+ and glutamate" Neurosci.Res.(Supplment). 14. S48 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Naoshi Fujuwara et al.: "Changes in intracellular pH of mouse hippocampal slices responding to hypoxia and/or glucose depletion" Brain Res.572. 335-339 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshio TAKAHATA et al.: "Anti-ischemic activity following minor brain injury -Effects of permanent ischemia model and sub-type of the mouse (in Japanese)" Sosei. 6. 92 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Naoshi FUJIWARA et al.: "The effect of nerve growth factor (NGF) on survival rate of mice following brain ischemia (in Japanese)" Masui. 38. S174 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Koki SHIMOJI et al.: "Protective effect of microinjury in brain ischemic damage" Advances in Brain Resuscitation. Springer-Verlag Tokyo. 115-121 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Koki SHIMOJI et al.: "Experimental ischemic model using brain slices (in Japanese)" Byoutai seiri. 10. 343-350 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi ABE et al.: "Simultaneous recording of[Ca^<2+>]_iand population potential in the hippocampal slice during hypoxia" Neurosci. Res.14(Suppl.). S19 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Naoshi FUJIWARA et al.: "Changes in intracellular pH of mouse hippocampal slices induced by high K^+ and glutamate" Neurosci. Res.14(Suppl.). S48 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Naoshi FUJIWARA et al.: "Changes in intracellular pH of mouse hippocampal slices responding to hypoxia and/or glucose depletion" Brain Res.572. 335-339 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Koki Shimoji,et al.: "Protective effect of microinjury in brain ischemic damage" Advances in Brain Resuscitation. 115-121 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 下地 恒毅ら: "脳組織切片を用いた脳虚血実験法" 病態生理. 10. 343-350 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takashi Abe et al.: "Simultaneous recording of [Ca2+]i and population potential in the hippocampal slice during hypoxia" Neurosci.Res.(Supplment). 14. S19 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Naoshi Fujiwara et al.: "Changes in intrecellular pH of omuse hippocampal slices induced by high K+ and glutamate" Neurosci.Res.(Supplment). 14. S48 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Naoshi Fujiwara et al.: "Changes in intracellular pH of mouse hippocampal slices responding to hypoxia and/or glucose depletion" Brain Res.572. 335-339 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 遠藤 裕: "海馬スライス標本における細胞内遊離Ca^<2+>濃度の変化ー低酸素・無グルコ-ス刺激" 麻酔. 39. S418 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] KoKi,Shimoji: "Hypoxia in brain slices" Biomed.Biochim.Acta. 48. S149-154 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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