• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Immunohistochemical and Electron Microscopic Study on the Somatosensory Receptive System in Orofacial Region

Research Project

Project/Area Number 63440069
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionNiigata University

Principal Investigator

MAEDA Takeyasu (1990)  Niigata University School of Dentistry, Assistant, 歯学部, 助手 (40183941)

小林 茂夫 (1988-1989)  新潟大学, 歯学部, 教授 (40018403)

Co-Investigator(Kenkyū-buntansha) SATO Osamu  Niigata University School of Dentistry, Assistant, 歯学部, 助手 (80202092)
須藤 弘幸  新潟大学, 歯学部, 助手 (70206572)
前田 健康  新潟大学, 歯学部, 助手 (40183941)
吉田 重光  新潟大学, 歯学部, 助教授 (80174928)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥28,700,000 (Direct Cost: ¥28,700,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1989: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1988: ¥25,000,000 (Direct Cost: ¥25,000,000)
KeywordsInnervation / Dental pulp / Periodontal ligament / Immunohistochemistry / Ruffini ending / Neurofilament protein / Calcitonin geneーrelated peptide (CGRP) / Nerve growth factor receptor / ルフィニ小体 / 立体構築 / CGRP / 顎関節 / 矯正力 / ラット歯根膜 / Ruffini神経終末 / terminal Schwann cell / nonーspecific cholinesterase / neurobilament protein / 歯髄神経 / 窩洞形成 / ラット / 神経終末 / ニューロフィラメントプロテイン / S-100蛋白 / 微細構造
Research Abstract

The findings obtained from this study are summarized as follows.
1. A major population of pulpal nerves in human teeth showed an intense nerve growth factor receptor (NGFR)-immunoreactivity. Immunoelectron microscopy demonstrated that the NGFR-immunoreactivity was localized on the axoplasmic membrane in both unmyelinated and naked axons, but not in myelinated fibers. A positive immunoreaction was also found in Schwann cells in unmyelinated fibers. Fibroblasts, cellular elements other than axons and some of Schwann cells were immunonegative.
2. Despite variation in development among species, Ruffini endings are accepted as essential mechanoreceptors, sensitive to tension of collagen fibers, in the periodontal ligament. Ruffini endings in the periodontal ligament were associated with terminal Schwann cells whose cytoplasm contained wellーdeveloped rough endoplasmic reticulum and Golgi apparatus. In addition to ordinary functions of supporting and nourishing of axon terminals, these cells we … More re able to synthesize cholinesterase and proteoglycans.
3. The response of pulpal nerves to dentin injury was investigated in rat molars by immunostaining for neurofilament protein. The distribution and shape of pulpal nerves reacted dynamically in response to cavity preparations, indicating possible involvement of the pulpal nerves in the tissue repair process following cavity preparations.
4. The response of the periodontal nerve fibers to orthodontic tooth movement was investigated in rat molars by use of immunohistochemistry for calcitonin geneーrelated peptide (CGRP). The dynamic changes of CGRP-immunoreactive nerves in the periodontal ligament was recognized during the tooth movement. It is suggested that the periodontal nerves are involved in inducing pain sensation in patients.
5. Although the presence of a variety of mechanoreceptors in the temporomandibular joint has been suggested by electrophysiological studies, no specialized nerve terminals except free nerve endings were observed in this immunohistochemical study. Less

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] O. Sato et al.: "Innervation of periodontal ligament and dental pulp in the rat incisor:An immunohistochemical investigation of neurofilament protein and gliaーspecific Sー100 protein." Cell and Tissue Research. 251. 13-21 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O.Sato et al.: "Filiform papillae as a sensory apparatus in the tomgue:An immunohistochemical study of nervous elements by use of neurofilament protein(NFP)and Sー100 protein antibodies." Cell and Tissue Research. 252. 231-238 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Maeda et al.: "Innervation of the periodontal ligament in rat incisors and molars:An immunohistochemical and electron microscopic study." Recent Advances in Clinical Periodontology. 309-312 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 前田 健康 ほか: "歯髄の神経支配 ーとくに自律神経とペプチド作動性神経について" 医学のあゆみ. 148. 629-631 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Maeda et al.: "The ultrastrture of Ruffini endings in the per;odontal ligament of rat incisors with special reference to the terminal Schwann cells(Kーcells)." The Anatomical Record. 223. 95-103 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O.Sato et al.: "Innervation of the incisors and periodontal lihgment in several rodents:An immunohistochemical study of neurofilament protein and gliaーspecific Sー100 protein." Acta Anatomica. 134. 94-99 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O.Sato: "Responses of pulpal nerves to cavity preparation in rat molars:An immunohistochemical study using neurofilament protein(NFP)antiserum" Archives of Histology and Cytology. 52. 433-446 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Maeda et al.: "Nerve terminals in human periodontal ligament as demonstrated by immunohistochemistry for neurofilament protein(NFP)and Sー100 protein." Archives of Histology and Cytology. 53. 259-265 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Maeda et al.: "Cholineserase activity in terminal Schwann cells associated with Ruffini nerve endings in the periodontal ligament of rat incisors." The Anatomical Record. 228. 339-344 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O.Sato et al.: "Immunohistochemical demonstration of nerve growth factor receptor in human teeth." Biomedical Research. 11. 353-358 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Kannari: "Sensory receptors in the periodontal ligament of hamster incisors with special reference to the distribution,ultrastructure and threedimensional reconstruction of Ruffini endings." Archives of Histology and Cytology. 53. 559-573 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 斉藤 功 ほか: "実験的歯牙移動時におけるラット臼歯歯根膜内神経線維の免疫組織化学的研究" 日本矯正歯科学会雑誌. 49. 466-474 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 前田 健康 ほか: "歯根膜感覚受容器の形態" 歯科ジャ-ナル. 33. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O. Sato et al.: "Innervation of periodontal ligament and dental pulp in rat incisors : An immunohistochemical investigation of neurofilament protein in glia-specific Sー100 protein." Cell Tissue Res.251. 13-21 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O. Sato et al.: "Filiform papillae as a sensory apparatus in the tongue : An immunohistochemical study of nervous elements by use of neurofilament protein (NFP) and S-100 protein antibodies." Cell Tissue Res.252. 231-238 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Maeda et al.: "Innervation of the periodontal ligament in rat incisors and molars : An immunohistochemical and electron microscopic study." Recent advances in clinical periodontology. 309-312 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Maeda et al: "Innervation of dental pulp, with special reference to autonomic and peptidergic nerves." Igakunoayumi. 148. 629-631 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Maeda et al: "The ultrastructure of Ruffini endings in the periodontal ligament of rat incisors with special reference to the terminal Schwann cells (K-cells)." Anat. Rec.223. 95-103 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O. Sato et al.: "Innervation of the incisors and periodontal ligament in several rodents : An immunohistochemical study of neurofilament protein and gliaーspecific S-100 protein." Acta Anat.134. 94-99 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O. Sato: "Responses of pulpal nerves to cavity preparation in rat molars : An immunohistochemical study using neurofilament protein (NFP) antiserum." Arch. Histol. Cytol.52. 433-446 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Maeda et al.: "Nerve terminals in human periodontal ligament as demonstrated by immunohistochemistry for neurofilament protein (NFP) and S-100 protein." Arch. Hystol. Cytol.53. 259-265 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Maeda et al.: "Cholinesterase activity in terminal Schwann cells associated with Ruffini nerve endings in the periodontal ligament of rat incisors." Anat. Rec.228. 339-344 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O. Sato et al.: "Immunohistochemical demonstration of nerve growth factor receptor in human teeth." Biomed. Res.11. 353-358 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Kannari: "Sensory receptors in the periodontal ligament of hamster incisors with special reference to the distribution, ultrastructure and threeーdimensional reconstruction of Ruffini endings." Arch. Hystol. Cytol.53. 559-573 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] I. Saito et al.: "An immunohistochemical study on the response of nerve fibers in the periodontium of rat molars during experimental tooth movement." J. Jpn. Orthod. Ass.49. 466-474

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Maeda et al.: "Nerve terminals in human periodontal ligament as demonstrated by immunohistochemistry for neurofilament protein (NFP) and Sー100 protein." Archives Histology and Cytology. 53. 259-265 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Maeda et al.: "Cholinesterase activity in terminal Schwann cells associated with Ruffini nerve endings in the periodontal ligament of rat incisors." The Anatomical Record. 228. 339-344 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] O.Sato et al.: "Immunohistochemical demonstration of nerve growth factor receptor in human teeth." Biomedical Research. 11. 353-358 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Kannari: "Sensory receptors in the periodontal ligament of hamster incisors with special refernce to the distribution,ultrastructure and threeーdimensional reconstruction of Ruffini endings." Archives of Histology and Cytology. 53. 559-573 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 斉藤 功ほか: "実験的歯牙移動時におけるラット臼歯歯根膜内神経線維の免疫組織化学的研究" 日本矯正歯科学会雑誌. 49. 466-474 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 前田 健康ほか: "歯根膜感覚受容器の形態" 歯科ジャ-ナル. 33. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Maeda et al.: "The ultrastructure of Ruffini endings in the periodontal ligament of rat incisors with special reference to the terminal Schwann cells(Kーcells)." The Anatomical Record. 223. 95-103 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] O.Sato et al.: "Innervation of the incisors and periodontal ligament in several rodents:An immunohistochemical study of neurofilament protein and gliaーspecific Sー100 protein" Acta Anatomica. 134. 94-99 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] O.Sato: "Resonses of pulpal nerves to cavity preparation in rat molars:An immunohistochemical study using neurofilament protein(NEP)antiserum." Archives of Histology and Cytology. 52. 433-446 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Maeda et al.: "Cholinesterase activity in terminal Schwann cells associated with Rrffini endings in the periodontal ligament of rat incisors." The Anatomical Record. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Maeda et al.: "Nerve terminals in human periodontal ligament as demonstrated by immunohistochemistry for neurofilament protein(NFP)and Sー100 protein." Archives of Histology and Cytology. 53. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Maeda;et al.: Recent Advances in Clinical Periodontology.In:J.Ishikawa et al.(eds.)Elsevier Science Publishers B.V.309-312 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Maeda;et al.: The Anatomical Record. 223. 95-103 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] O.Sato;et al.: Acta Anatomica. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] O.Sato: Archives of Histology and Cytology. 51. (1989)

    • Related Report
      1988 Annual Research Report

URL: 

Published: 1988-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi