| Project/Area Number |
63440083
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
NOSAKA Tetsuya (1991) Inst. Virus Res, Kyoto University Instructor, ウイルス研究所, 助手 (30218309)
安達 喜文 (1990) 京都大学, 医学部, 助手 (50201893)
村地 孝 (1988-1989) 京都大学, 医学部, 教授 (10089104)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Kunihiro Kyoto Univ. Fac. Med. Associate Prof., 医学部, 助教授 (00027070)
HATANAKA Masakazu Inst. Virus Res. Kyoto Univ. Professor, ウイルス研究所, 教授 (30142300)
MAKI Masatoshi Inst. Virus Res. Kyoto Univ. Associate Prof, ウイルス研究所, 助教授 (40183610)
ADACHI Yoshifumi Kyoto Univ. Fac. Med. Instructor, 医学部, 助手 (50201893)
MURACHI Takashi Kyoto Univ. Fac. Med. Professor, 医学部, 教授 (10089104)
野阪 哲哉 京都大学ウイルス研究所, 助手 (30218309)
神奈木 玲児 京都大学, 医学部, 講師 (80161389)
戸谷 誠之 京都大学, 医学部, 講師 (70163988)
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| Project Period (FY) |
1988 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥31,400,000 (Direct Cost: ¥31,400,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1989: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1988: ¥20,700,000 (Direct Cost: ¥20,700,000)
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| Keywords | Calpain / Calpastatin / Proteinase / Inhibitor / HTLV-I / 遺伝子発現 / ELISA / cDNA / mRNA / 病因病態解析 / モノクロ-ナル抗体 / 酵素免疫測定法 / 臨床検査技術 |
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| Research Abstract |
Calpain is an intracellular Ca^<2+>-dependent cysteine endopeptidase, and calpastatin is an endogenous specific inhibitor of calpain. Both proteins constitute an intracellular regulatory system operating in response to Ca^<2+> signaling. We cloned the CDNA for pig heart calpastatin and investigated structure-function relationships. The inhibitor consists of four inhibitory domains with mutually homologous sequences. A human calpastatin genomic DNA clone- was isolated, and synthetic oligopeptide corresponding to an exon of the human calpastatin gene showed strong inhibition against calpain I and calpain. When various hematopoietic cells were screened by Westem blot analysis, using antibodies specific for calpain I and for calpain 11, respectively ; HTLV-L infected cells were found to express high level ofcalpain 11, whereas HTLV-l non infected T cells did not express any appreciable amount of calpain 11. Calpain I was expressed in all the cells tested. Calpastatin gene expression was also found to be elevated in HTLV-L infected cells.
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