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微生物細胞捕捉機能を有する高分子材料の合成及びバイオリアクタ-への応用

Research Project

Project/Area Number 63470100
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 高分子合成
Research InstitutionKYOTO INSTITUTE OF TECHNOLOGY

Principal Investigator

KAWABATA Nariyoshi  KYOTO INSTITUTE OF TECHNOLOGY PROFESSOR, 工芸学部, 教授 (70025998)

Co-Investigator(Kenkyū-buntansha) FURUYOSHI Setuo  KYOTO INSTITUTE OF TECHNOLOGY ASSISTANT, 工芸学部, 助手 (70199446)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥5,500,000 (Direct Cost: ¥5,500,000)
Keywordspyridinium-type resin / microbial cells / immobilization by capture / bioreactor / cortisol / prednisolone / ammonium fumarate / L-aspartic acid / バイオリアクター
Research Abstract

The head investigator has found a new functional polymer, insoluble pyridiniumtype resin, which captures microorganisms alive. The purpose of this research project was to develop a new method of immobilization of microbial cells based on the resin, and to apply for bioreactor. In academic year of 1988, whole cells of Escherichia coli with aspartase activity were immobilized by capture on the surface of the resin. When a suspension of the bacterial cells in buffer solution was passed through a glass column containing beads of the resin, the cells were captured on the surface and formed an immobilized cell system, which was used for the preparation of L-aspartic acid from-ammonium fumarate. Continuous operation of a fixed-bed column reactor containing the unmobilized . bacterial cells produced L-aspartic acid in a quantitative yield when the influent substrate concentration was 0.1 M and the flow rate was 0.41-0.83 bed volumes per hour at pH 7.4-7.7 at 30゚C. In academic year of 1989, cells of Arthrobacter simplex having 3-ketosteroid-DELTA^1-dehydrogenase activity were immobilized by capture on the surface of unwoven cloth coated by pyridinium-type polymer. The captured cells catalyzed the transformation of cortisol to prednisolone in methanolic solvents. Continuous operation of a fixed-bed column reactor gave prednisolone in about 90% yield when the influent cortisol concentration was 1.0 mmol/1 and the flow rate was 0.57 bed volumes per hour at 20゚C and at pH 8.2.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] N.Kawabata: "Increase of Bacterial Popxlation by Cross-Linked Polyvinylpyridine Added to a Model System for Continuous Aerobic Treatment" Journal of Fermentation and Bioengineering. 68. 433-436 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N.Kawabata: "New Method of Immobilization of Microbial Cells by Capture on the Surface of Insoluble Pyridinium-Type Resin" Biotechnology and Bioengineering. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Kawabata, M. Oda: "Improvement of Aerobic Treatment Process by Cross-Linked Polyvinylpyridine" Biotechnology and Bioengineering, 34, 1045-1049 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Kawabata, M. Tachibana: "Increase of Bacterial Population by Cross-Linked Polyvinylpyridine Added to a Model System for Continuous Aerobic Treatment" Journal of Fermentation and Bioengineering, 68, 433-436 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Kawabata, S. Nishimura, T. Yoshimura: "New Method of Immobilization of Microbial Cells by Capture on the Surface of Insoluble Pyridinium-Type Resin" Biotechnology and Bioengineering, 35, (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N.Kawabata: "Increase of Bacterial Popxlation by Cross-Linked Polyvinylpyridine Added to a Model System for Continuous Aerobic Treatment" Journal of Fermentation and Bioengineering. 68. 433-436 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Kawabata: "New Method of Immobilization of Microbial Cells by Capture on the Surface of Insoluble Pyridinium-Type Resin" (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Kawabata,; M.Oda: Biotechnology and Bioengineering. (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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