| Project/Area Number |
63480017
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物形態・分類学
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| Research Institution | University of Osaka Prefecture |
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Principal Investigator |
MOMOTANI Yoshihide Univ. Osaka Pref., Coll. Integrated Arts & Sci., Prof., 総合科学部, 教授 (60079069)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Kensuke Univ. Osaka Pref., Assistant prof., 総合科学部, 助手 (10209942)
UEDA Kunihiko Univ. Osaka Pref., Assistant Prof., 総合科学部, 助手 (60184925)
UEDA Junichi Univ. Osaka Pref ., Associate Prof., 総合科学部, 助教授 (40109872)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1988: ¥4,700,000 (Direct Cost: ¥4,700,000)
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| Keywords | essential oil / chemotaxonomy / Magnoliaceae / molecular phylogeneic tree / isozyme / physiological activity / 木本性多心皮群 / モクレン属 / ザイモグラム / 精油 / 分子系統学 |
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| Research Abstract |
The fresh leaves of the Magnoliaceae have many kinds of the essential oil components more than one hundred. Since the essential oil is generally considered to be a taxonomic character, the fresh leaves of three sections of subgen. Magnolia and Yulania of the genus Magnolia and those of Liriodendrion were collected for the chemotaxonomic study using their essential oils. Essential oils were isolated by the procedure of the steam distillation, and purified with the solvent fractionation using diethyl ether. Each component of the essentialiol was identified with gas-liquid chromatography/mass spectrometry. The results in this study confirmed the taxonomic idea and/or considerations proposed by morphological studies. Furthermore, chemotaxonomic study using the essential oil is especially useful for the recognition of the hybrid that has not been identified by the data resulted from morphological studies.
Using isozyme technique based on naturally occurring enzyme variation, the molecular phylogenetic tree of the Magnoliaceae was proposed. The results supported the idea and/or considerations from the traditional taxonomy.
On the other hand, the study of physiological significance of the essential oil in the Magnoliaceae led to the successful identification of caryophyllene oxide as an inhibitor of cytokinin-induced retardation of oat leaf senescence and auxin- induced elongation of oat coleoptile segments.
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