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Light-Microscopic Analysis of Secretory Activity of Neurosecretory Cells

Research Project

Project/Area Number 63480024
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionUniversity of Tokyo

Principal Investigator

URANO Akihisa  Univ. Tokyo, Ocean Res. Inst. Prof., 海洋研究所, 教授 (00142232)

Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥6,300,000 (Direct Cost: ¥6,300,000)
KeywordsNeurosecretory cell / Neurohypophysial hormones / Gene expression / in situ hybridization / Water and mineral metabolisms / Voltage sensitive dye / 神経分泌系 / 神経ホルモン / ホルモン遺伝子 / ホルモンの合成 / ホルモンの分泌 / バソトシン / イソトシン
Research Abstract

In the vertebrates, the hypothalamo-neurohypophysial neurosecretory system is involved in regulation of various physiological phenomena. However, it is not yet clear how synthesis and release of neurohypophysial hormones are controlled by external and internal environmental changes. Thus, in this research project, I have attempted to study this questions by use of two dimensional light microscopic photometric techniques, Synthetic activity of neurohypophysial hormones in individual neurosecretory cells has been determined by semiquantitative immunohistochemical and oligonucleotide-mRNA in situ hybridization (ISH) techniques. The ISH method used in the present project was established and developed in our laboratory. Furthermore, the nucleotide sequences of cDNAs encoding precursors of salmonid neurohypophysial hormones were analyzed in chum salmon and masu salmon so as to examine the above questions from comparative bases.
Concerning effects of environmental osmotic changes on expression … More s of neurohypophysial hormone precursor genes, we have been able to obtain following results :
1) After drinking 2% NaCl solution in rats, the level of provasopressin mRNA was significantly elevated in the supraoptic nucleus, but the change in the paraventricular nucleus was not significant. While water deprivation increased the provasopressin mRNA level in both the supraoptic and paraventricular nuclei.
2) Transfer of seawater-adapted rainbow trout to fresh water elevated the level of provasotocin mRNA, whereas the inverse lowered the mRNA level.
3) These results indicate that expression of genes encoding vasopressin-like peptides is elevated by a hyperosmotic stimulus in the rat, whereas it is stimulated by a hypoosmotic stimulus in the salmonid fish.
Application of voltage-sensitive fluorescent dyes has been tested to record discharges of individual neurosecretory cells, since secretory activity of neurohypophysial hormones is well correlated to neuronal electric activity. I have not been able to complete this part, because of a delay in preparation of computer programs used in this technique. Less

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Hyodo,S.: "Development of an in situ hybridization method for neurohypophysial hormone mRNAs using synthetic oligonucleotide probes." Zooiogical Science. 5. 397-406 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hyodo,S.: "Molecularー and immunoーhistochemical study on expressions of vasopressin and oxytocin genes following sodium loading." Zoological Science. 5. 1033-1042 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hyodo,S.: "Molecularー and immunoーhistochemical study on expressions of vasopressin and oxytocin genes following water deprivation." Zoological Science. 6. 335-343 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Urano,A.: "In situ hybridization techniques in the study of endocrine secretions." Progress in Clinical and Biological Research. 342. 309-314 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hyodo,S.: "Cloning and sequence analyses of cDNAs encoding vasotocin and isotocin precursors of chum salmon,Oncorhynchus Keta:evolutionary relationships of neurohypophysial hormone precursors." Journal of Comparative Physiology B. 120. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsumoto,A.: "Cellular localization of gap junction mRNA in the neonatal rat brain." Neuroscience Letters. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hyodo, S.,: "Development of an in situ hybridization method for neurohypophysial hormone mRNAd using synthetic oligonucleotide probes." Zoological Science. 5. 397-406 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hyodo, S.,: "Molecular- and immuno-histochemical study on expressions of vasopressin and oxytocin genes following sodium loading." Zoological Science. 5 :. 1033-1042 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hyodo, S.,: "Moleculer- and immuno-histochemical study on expressions of vasopressin and oxytocin genes following water deprivation." Zoological Science. 6 :. 335-343 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Urano, A.,: "In situ hybridization techniques in the study of endocrine secretions." Progress in Clinical and Biological Research. 342. 309-314 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hyodo, S.,: "Cloning and sequence analyses of cDNAs encoding vasotocin and isotocin precursors of chum salmon, Oncorhynchus keta : evolutionary relationships of neurohypophysial hormone precursors." Journal of Comparative physiology B. 120. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsumoto , A.,: "Cellular localization of gap junction mRNA in the neonatal rat brain." Neuroscience Letters. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Urano,A.: "In situ hybridization techniques in the study of endocrine secretion." Progress in Clinical and Biological Research. 342. 309-314 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hyodo,S.: "Cloning and sequence analyses of cDNAs encoding vasotocin and isotocin precursors of chum salmon,Onchorhynchus keta:evolutionary relationships of neurohypophysial hormone precursors." Journal of Comparative Physiology B. 120. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsumoto,A.: "Cellular localization of gap junction mRNA in the neonatal rat brain." Neuroscience Lefters. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hyodo,S.: "Changes in expression of provasotocin and proisotocin genes during adaptation to hyperー and hypoーosmotic environments in rainbow trout." Journal of Comparative Physiology B.

    • Related Report
      1990 Annual Research Report
  • [Publications] Hyodo,S.: "Expression of provasopressin gene during ontogeny in the hypothalamus of developing mice." Neuroscience.

    • Related Report
      1990 Annual Research Report
  • [Publications] S・Hyody: "Molecular-and immuno-histochemical study on expressions of vasopressin and oxytocin genes following water deprivation" Zoological Science. 6. 335-343 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Hyodo: "Cloning and sequence analysis of cDNAs for neurohypophysial hormone vasotocin of chum salmon,Oncorhynchus keta" Proc.Japan Society for Comparative Endocrinology. 4. (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Urano: "In situ hybridization techniques in the study of endocrine secretion" Progress in Comparative Endocrinology、1989. (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Hyodo;M.Fujiwara;S.Kozono;M.Sato;A.Urano: Zoological Science. 5. 397-406 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Hyodo;M.Fujiwara;M.Sato;A.Urano: Zoological Science. 5. 1033-1042 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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