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Molecular Genetic Research on Organellar Specific Synthesis and Intracellular Translocation of Membrane Phospholipids.

Research Project

Project/Area Number 63480054
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionSaitama University

Principal Investigator

OHTA Akinori  Saitama Univ., Biochemistry, Assoc. Prof., 理学部, 助教授 (30125885)

Co-Investigator(Kenkyū-buntansha) MATSUZAKI Hiroshi  Saitama Univ., Biochemistry, Lecturer, 理学部, 助手 (80008870)
SHIBUYA Isao  Saitama Univ., Biochemistry, Prof., 理学部, 教授 (60013306)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1988: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsPhospholipid / Phosphatidylserine / Phosphatidylethanolamine / Oranelle / Saccharomyces cerevisiae / Mitochondrion / Microsome / Yeast / リン脂質 / ホスファチジルセリンシンタ-ゼ / ホスファチジルセリンデカルボキシラ-ゼ / CHO1 / Schizosaccharomyces pombe / オルガネラ / 酵素局在性 / ホスファチジルセリンシンターゼ / ホスファチジルセリンデカルボキシラーゼ / リン脂質合成
Research Abstract

1) Phosphatidylserine (PS) synthase as well as phosphatidylinositol (PI) synthase has been formerly assumed to be localized in bothmitochondria and microsome in Saccharomyces cerevisiae. Our density gradient centrifugation analyses of crude cell extracts, however, revealed that the mitochondrial localization of these two enzymes is unlikely.
2) We constructed a series of chol'-lacZ fusion genes and examined subcellular localization of their products. The fusion proteins with PS synthase N-terminals of more than 129 amino acids were found to be distributed among subcellular fractions in the similar manner with the PS synthase, whereas those with PS synthase N-terminals of less than 114 amino acids were found in soluble fraction. This result suggests that the region between 114th to 129th residue, which is rich in hydrophobic amino acids, is of critical importance for microsomal binding of PS synthase.
3) We obtained two mutants with lowered PS decarboxylase activity, one of which carried a mutation that could be classified to pet and was defective in PS decarboxylase activity when it was grown in the presence of myo-inositol.
4) The PS decarboxylase activity of a fission yeast Schizosaccharomyces pombe was less than one-tenth of that of S. cerevisiae. This and other lines of evidence suggest that decarboxylation of PS does not so much contribute to phosphatidylethanolamine formation in the fission yeast as known in the budding yeast.

Report

(3 results)
  • 1990 Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Takeshi Hikiji: "Disruption of the CHO1 gene encoding phosphatiylserine synthsae in Saccharomyces cerevisiae." JournaL of Biochemistry. 107. 894-900 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 太田 明徳: "生体膜の遺伝子工学" Radioisotopes. 37. 535-543 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 太田 明徳: "リン脂質合系酵素の細胞内局在性と機能調節" 遺伝. 43. 28-33 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miyuki Fukushima: "Subcelluar localization of synthetic actitvities for phosphatidylーserine and phosphatidylinositol in Saccharomyces cerevisiae." FEBS Letters.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yu Fukuda: "Structural prerequisites of yeast phosphatidylserine synthase for its microsomal localization." To be submitted.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 太田 明徳(共著): "学会出版センタ-" 酵母における膜アミノリン脂質の生合成(酵母のバイオサイエンスー伝統と新展開ー栃倉展六郎編), 12 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takeshi Hikiji: "Disruption of the CHO1 gene encoding phosphatidylserine synthase in Saccharomyces cerevisiae." Journal of Biochemistry. Vol. 104. 894-900 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miyuki Fukushima: "Subcellular distribution of synthetic activities for phosphatidylserine and phosphatidylinositol in Saccharomyces cerevisiae." Journal of Biochemistry.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yu Fukuda: "Structural prerequisites of yeast yeast phosphatidylserine synthase for its microsomal localization."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yu Fukuda: "Subcellular localization of phosphatidylserine synthase with mitochondrial targeting signal on its amino terminus."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akinori Ohta: "Gene technology of biomembrane (in Japanese)." Vol. 37. 535-543 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akinori Ohta: "Intracellular localization and regulation of phospholipid-synthesizing enzymes (in Japanese)." Radioisotopes,. Vol. 43. 28-33 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akinori Ohta: "Biosynthsis of membrane amino-phospholipids (in Japanese)." 'Bioscience of Yeast' Ed. S. Tochikura, Gakkai Shuppan Center. 57-68 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takeshi Hikiji: "Disruption of the CHO1 gane encoding phosphatidylserine synthase in Saccharomyces cerevisiae." Journal of Biochemistry. 104. 894-900 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Akinori Ohta: "Subcellular localization of phoshatidylserine synthase in Saccharomyces cerevisiae." Proceedings of the 29th International Conference on the Biochemistry of Lipids.144 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 太田明徳: "「遺伝子工学実験講座」XI.生体膜の遺伝子工学" Radioisotopes. 37. 535-543 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 太田明徳: "リン脂質合成系酵素の細胞内局在性と機能調節" 遺伝. 43. 28-33 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yohko Asai: "Suppression of the lethal effect of Acidic-phospholipid deficiency by defective formation of the major outer membrane lipoprotein in Escherichia coli." Journal of Bacteriology. 171. 6867-6869 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Miyuki Fukushima: "Subcellular distribution of synthetic activities for aminophospholipids and phosphatidylinositol in yeast is more like that in higher eukaryotes." Journal of Biochemistry.

    • Related Report
      1989 Annual Research Report
  • [Publications] 太田明徳: "「酵母のバイオサイエンス:伝統と新展開」河合弘康編6.酵母における膜アミノリン脂質の生合成" 学会出版センタ-, (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takeshi,Hikiji: Journal of Biochemistry. 104. 894-900 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Akinori,Ohta: Proceedings of 29th International Conference on the Bio-chemistry of Lipids. 144 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 太田明徳: Radioisotopes. 37. 535-543 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 太田明徳: "「酵母のバイオサイエンス:伝統と新展開」酵母における膜アミノリン脂質の生合成" 学会出版センター, (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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